MicroRNA Expression Profiling of Bone Marrow Mesenchymal Stem Cells in Steroid-Induced Osteonecrosis of the Femoral Head Associated with Osteogenesis

Abstract

BackgroundSteroid-induced osteonecrosis of the femoral head (SONFH) is a common orthopedic disease associated with the application of glucocorticoid (GC). In this study, we detected the microRNAs (miRNAs) differentially expressed in bone marrow mesenchymal stem cells (BMSCs) from SONFH patients, and target gene predictions were performed, and the functions of the target genes was verified.Material/MethodsBMSCs collected from patients with SONFH and femoral neck fracture (FNF) constituted the SONFH group (n=3) and FNF (control) group (n=3), respectively. MiRNA microarray analysis was utilized to detect the differentially expressed miRNAs, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the microarray results. The target genes and functions of the differentially expressed miRNAs were analyzed using a bioinformatics database.ResultsThe microarray results revealed that compared with the control group, 22 miRNAs were identified differentially expressed in the SONFH group, with 17 upregulated and 5 downregulated. Further qRT-PCR validation of differentially expressed miRNAs confirmed that hsa-miR-601, hsa-miR-452-3p, hsa-miR-647, and hsa-miR-516b-5p were significantly increased, whereas hsa-miR-122-3p was significantly decreased. During osteogenic differentiation, hsa-miR-601, hsa-miR-452-3p, hsa-miR-647, hsa-miR-516b-5p, and hsa-miR-127-5p were significantly downregulated, whereas hsa-miR-122-3p was significantly upregulated, and miRNAs showed a converse tendency during adipogenic differentiation.ConclusionsSix miRNAs associated with osteogenic and adipogenic differentiation were identified differentially expressed in the BMSCs of SONFH patients; these miRNAs may serve as novel biomarkers or therapeutic targets for SONFH.

Roles for miRNAs in osteogenic differentiation of bone marrow mesenchymal stem cells

“…Twenty-two differentially expressed miRNAs were identified in bone marrow mesenchymal stem cells (BMSCs) from patients with steroid-induced femoral head necrosis, 17 were upregulated and five were downregulated. During osteogenic differentiation of BMSCs, hsa-mir-601, hsa-mir-452-3p, hsa-mir-647, hsa-mir-516b-5p, and hsa-mir-127-5p were significantly downregulated, while hsa-mir-122-3p was significantly upregulated, suggesting that different miRNAs promoted or inhibited osteogenic differentiation [19]. miRNAs and their identified target genes in the osteogenic differentiation of BMSCs are summarized in Fig.…”

Noncoding RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

“…In addition, the expression level of hsa-miR-122-3p in the group at Ficat stage IV was found to be lower than that in the stage III group, suggesting that the expression level of hsa-miR-122-3p in BMSCs may be correlated with the progression of steroid-induced ONFH. Wang et al [13] profiled the miRNA expression in three patients with steroid-induced ONFH and three patients with femoral neck fracture and identified 17 upregulated and five downregulated miRNAs in the steroid-induced ONFH group. Moreover, in an in vitro study, they identified five miRNAs (miR-601, miR-452-3p, miR-647, miR-516b-5p, and miR-127-5p) with decreased levels and one miRNA (miRNA-122-3p) with an increased level during osteogenic differentiation, whereas the changes in the expression levels during adipogenic differentiation were in the opposite direction.…”

Trans-Cervical Femoral Fractures in Young Adults: The Prognosis is not only in the Hands of Orthopaedic Surgeons

“…As we all know, this nail is theoretically contra-indicated for cervical femoral fractures due to the rotational forces applied on the femoral head during the insertion of the lag screw. These forces lead to severe and inacceptable rotation of the fracture site and usually displace the fracture in a pejorative manner [24][25][26][27].…”