microRNA Expressions in CD4+ and CD8+ T-cell Subsets in Autoimmune Thyroid Diseases

Bernecker, C.; Halim, Faridah Abdul; Lenz, Luisa; Haase, Matthias; Nguyen, Thi Hiep; Ehlers, Margret; Vordenbaeumen, S.; Schott, M.

doi:10.1055/s-0033-1361088

Cited by 28 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phenomenon that miRNA expression profile from peripheral blood is different from that of diseased tissue is also found in other diseases, such as IBD, RA, SLE and psoriasis [22,32,33,34,35,36,37,38]. In addition, our results are also different from study done by Bernecker et al, in which they confirmed significant variations of miR-200a and miR-155 in CD4+ T-cells and CD8+ T-cells from AITD patients [39]. The reason for this divergence not only due to the different materials but also the different methods.…”

Section: Discussioncontrasting

confidence: 99%

Aberrant Expression of miRNA and mRNAs in Lesioned Tissues of Graves' Disease

Qin

Wang

et al. 2015

Cell Physiol Biochem

View full text Add to dashboard Cite

Background and Aims: Abnormal microRNA (miRNA) expression is found in many diseases including autoimmune diseases. However, little is known about the role of miRNA regulation in Graves' disease (GD). Here, we simultaneously detected different expressions of miRNA and mRNAs in thyroid tissues via a high-throughput transcriptomics approach, known as microarray, in order to reveal the relationship between aberrant expression of miRNAs and mRNAs spectrum and GD. Methods: Totally 7 specimens of thyroid tissue from 4 GD patients and 3 controls were obtained by surgery for microarray analysis. Then, 30 thyroid specimens (18 GD and 12 controls) were also collected for further validation by quantitative real-time PCR ( qRT-PCR ). Results: Statistical analysis showed that the expressions of 5 specific miRNA were increased significantly while those of other 18 miRNA were decreased in thyroid tissue of GD patients (FC≥1.3 or≤0.77 and p<0.05). In addition, the transcription of 1271 mRNAs was up-regulated, while the expression of 777 mRNAs transcripts was down-regulated (FC≥2.0 or≤0.5 and p<0.05). Furthermore, integrated analysis of differentially expressed miRNA and their target mRNAs demonstrated that 2 miRNA (miR-22 and miR-183) were increased while their potential target mRNAs were decreased. 3 miRNA (miR-101, miR-197 and miR-660) were decreased while their potential target mRNAs were increased. The above findings from microarray screening were confirmed by qRT-PCR in more samples. The results were consistent with those observed in the microarray assays. Conclusion: Our study highlights the possibility that miRNA-target gene network may be involved in the pathogenesis of GD and could provide new insights into understanding the pathophysiological mechanisms of GD.

show abstract

Section: Discussioncontrasting

confidence: 99%

Aberrant Expression of miRNA and mRNAs in Lesioned Tissues of Graves' Disease

Qin

Wang

et al. 2015

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…GD and HT patients had significantly lower levels of miR-146a-5p and miR-155-5p in the thyroid tissues, respectively (128). A subsequent study by Bernecker et al found that GD and HT patients had lower level of miR-155-5p in HT in CD8+ T cells than controls (130). Wei et al reported that Graves’ ophthalmopathy patients had significantly lower levels of miR-146a-5p than controls, and miR-146a-5p was negatively correlated with serum level of IL-17, which had been suggested to be an important pathogenic cytokine in the development of Graves’ ophthalmopathy (33, 34, 137).…”

Section: Epigenetics In Aitdmentioning

confidence: 99%

The Emerging Role of Epigenetics in Autoimmune Thyroid Diseases

et al. 2017

View full text Add to dashboard Cite

Autoimmune thyroid diseases (AITD) are a group of both B cell- and T cell-mediated organ-specific autoimmune diseases. Graves’ disease and Hashimoto thyroiditis are the two main clinical presentations of AITD. Both genetic and environmental factors have important roles in the development of AITD. Epigenetics have been considered to exert key roles in integrating those genetic and environmental factors, and epigenetic modifications caused by environmental factors may drive genetically susceptibility individuals to develop AITD. Recent studies on the epigenetics of AITD have provided some novel insights into the pathogenesis of AITD. The aim of this review is to provide an overview of recent advances in the epigenetic mechanisms of AITD, such as DNA methylation, histone modifications, and non-coding RNAs. This review highlights the key roles of epigenetics in the pathogenesis of AITD and potential clinical utility. However, the epigenetic roles in AITD are still not fully elucidated, and more researches are needed to provide further deeper insights into the roles of epigenetics in AITD and to uncover new therapeutic targets. Although there are many studies assessing the epigenetic modifications in AITD patients, the clinical utility of epigenetics in AITD remains poorly defined. More studies are needed to identify the underlying epigenetic modifications that can contribute to accurate diagnosis of AITD, adequate choice of treatment approach, and precise prediction of treatment outcomes.

show abstract

“…For example, it was observed that the expression of miR-154*, miR-376b, and miR-431* was suppressed in PBMC from initial GD patients with respect to healthy controls, but recovered in GD patients in remission (39). Others observed that serum levels of miR-22, miR-375, and miR-451 were increased in patients with HT compared with healthy subjects and that serum levels of miR-16, miR-22, miR-375, and miR-451 were increased in patients with GD (40), while another study revealed significant variations of miR-200a and miR-155 in purified CD4+ T-cells and CD8+ T-cells of patients suffering from GD and HT (41). More recent studies attempted to explain the biological significance of miRNA deregulation or their possible clinical implications in AITD (42–46).…”

Section: Non-coding Rnas In Aitdmentioning

confidence: 99%

Epigenetics and Autoimmune Thyroid Diseases

Coppedè

2017

Front. Endocrinol.

View full text Add to dashboard Cite

Increasing evidence suggests that epigenetic modifications, including changes in DNA methylation, covalent modifications of histone tails, and gene silencing mediated by non-coding RNA molecules, play a substantial role in the pathogenesis of autoimmune disorders and might be seen as the result of environmental insults that trigger these conditions. Studies in cells and tissues of patients with autoimmune thyroid diseases (AITD), and particularly in Graves’ disease (GD) and Hashimoto’s thyroiditis (HT), are increasingly revealing altered epigenetic marks and resultant deregulation of gene expression levels, but the available data are still limited to be translated into the clinical settings. Particularly, genome-wide methylation and histone tail modification screenings are limited to a few studies in GD patients, and the diagnostic values of the observed epigenetic changes or their potential prognostic utility are still unclear. Similarly, data concerning microRNA expression in AITD patients are largely descriptive and not yet translated into the clinics. In addition, studies relating certain environmental exposures to specific epigenetic changes in AITD and studies evaluating the crosstalk between different epigenetic mechanisms are largely missing. In summary, despite that there is a clear evidence of epigenetic impairment in AITD, further research is required for a better understanding of the epigenetic networks involved in disease pathogenesis, thereby opening the way for potential diagnostic and prognostic tools, as well as for epigenetic interventions in the patients.

show abstract

microRNA Expressions in CD4+ and CD8+ T-cell Subsets in Autoimmune Thyroid Diseases

Cited by 28 publications

References 35 publications

Aberrant Expression of miRNA and mRNAs in Lesioned Tissues of Graves' Disease

Aberrant Expression of miRNA and mRNAs in Lesioned Tissues of Graves' Disease

The Emerging Role of Epigenetics in Autoimmune Thyroid Diseases

Epigenetics and Autoimmune Thyroid Diseases

Contact Info

Product

Resources

About