MicroRNA in Sjögren’s Syndrome: Their Potential Roles in Pathogenesis and Diagnosis

Reale, Marcella; D’Angelo, Chiara; Costantini, Erica; Laus, Melissa; Moretti, Alex; Croce, A

doi:10.1155/2018/7510174

Cited by 48 publications

(41 citation statements)

References 80 publications

(106 reference statements)

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“…microRNAs (miRs) have been identified as the noncoding RNAs and found to participate in the progression and management of the inflammatory response [8]. Increasing evidence have elucidated that miRs are associated with inflammation in SS patients [9][10][11]. Besides, miR-377 has been found to mediate the proliferation and apoptosis progresses as well as DNA methylation in several malignancies [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

Xin

Liang

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background: The interaction of long non-coding RNAs (lncRNAs)-microRNAs (miRs) exerts crucial functions in mediating inflammatory reaction. It is still unclear whether myocardial infarction associated transcript 2 (Mirt2)-miR-377 mediates the inflammatory pathogenesis in Sj€ ogren's syndrome (SS). Methods: The inflammatory lesion model was established by stimulating salivary gland epithelial cells (SGECs) by interferon gamma (IFN-c). Mirt2-and/or miR-377-transfected SGECs, as well as their negative controls, were applied to investigate the biological functions in inflammation. Cell viability and apoptosis were examined using commercial kits. Western blot was applied to quantify protein level, and enzyme-linked immuno sorbent assay (ELISA) was used to value the secretion of cytokines. Results: The up-regulation of Mirt2 was observed in IFN-c-treated SGECs. Mirt2 overexpression restored the expression of miR-377 which was repressed by IFN-c. However, miR-377 silence abolished the protective effect on cell viability, inhibitory effect on apoptosis and prohibitive role in pro-inflammatory factors. Mirt2 diminished the phosphorylated expression of crucial regulators while miR-377 silence restored the phosphorylation in IFN-c-treated SGECs. Conclusion: Mirt2 was elevated in IFN-c-treated SGECs and then up-regulated miR-377 in response to inflammatory lesions. Mechanically, in synergy with miR-377 Mirt2 blocked IFN-c-evoked activation of NF-jB and JAK/STAT signalling pathway. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

Xin

Liang

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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show abstract

“…The human genome encodes approx. 2700 different miRNAs (http://www.mirbase.org), and one miRNA can influence the expression of multiple mRNAs, whereas one mRNA is usually regulated by several miRNAs that may act in concert 4 . The research field of miRNAs is rather new, and the exact biological functions of many miRNAs are not yet fully understood.…”

Section: Introductionmentioning

confidence: 99%

Distinct microRNA expression profiles in saliva and salivary gland tissue differentiate patients with primary Sjögren's syndrome from non‐Sjögren's sicca patients

Sembler-Møller

Belstrøm

Locht

et al. 2020

J Oral Pathology Medicine

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Objectives Increasing evidence suggests that aberrant expression of microRNAs (miRNAs) is involved in the pathogenesis of primary Sjögren's syndrome (pSS). The aim was thus to characterize the miRNA profile in saliva, salivary gland tissue, and plasma from patients with pSS and compare findings with those of patients having Sjögren‐like disease (non‐pSS). In addition, to correlate miRNA levels and clinicopathological features of pSS. Methods miRNA real‐time quantitative polymerase chain reaction was performed on saliva, plasma, and salivary gland tissue samples from 24 patients with pSS and 16 non‐pSS in 384‐well plates. T test was used for comparison of miRNA profiles, followed by Benjamini‐Hochberg correction. The discriminatory power of miRNAs was evaluated by receiver‐operating characteristic curves, and Pearson/Spearman correlation was used for correlation analyses. Results In saliva, 14 miRNAs were significantly differentially expressed between pSS and non‐pSS, including downregulation of the miR‐17 family in pSS. In salivary gland tissue of patients with pSS, miR‐29a‐3p was significantly upregulated. Plasma miRNAs did not differ between the two groups, although the miR‐17 family tended to be downregulated. The combination of miR‐17‐5p and let‐7i‐5p in saliva yielded an area under curve of 97% (CI 92%‐100%). Several miRNAs correlated significantly with one another and with salivary flow rates and histopathology. Conclusion Our findings indicate that the miRNA expression profile in saliva may enable to discriminate between pSS and non‐pSS patients. However, further validation in larger cohorts is needed as well as functional analyses of the miRNAs of interest.

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“…Now more and more research paid attention to miRNA in various diseases. Among them, the microRNA related to dry eye syndrome is a hot topic too [25]. And it could regulate numerous physiological processes via binding to the 3′ untranslatable region (3′-UTR) of their mRNA targets [26].…”

Section: Discussionmentioning

confidence: 99%

The expression of miRNA-146a-5p and its mechanism of treating dry eye syndrome

Yin

Zhang

et al. 2020

Preprint

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Background: Dry eye syndrome in which tear fluid quality or abnormality or kinetic abnormality is caused by many causes, resulting in decreased tear film stability. In recent years, more and more results from the studies indicate that miRNA alterations are involved in dry eye syndrome. And miRNA-146a-5p is a key regulator to regulate the inflammatory response. In this paper, we demonstrated whether miRNA-146a-5p could cure dry eye syndrome by regulating target genes based on network analysis.Methods: In this paper, we collected the blood of patients with dry eye disease which served as a model group, the blood of healthy people was used as a control group. The expression of miRNA-146a-5p in the patients was detected by RT-PCR, the genes controlled by miRNA-146a-5p were predicted by Targetscan, miRDB, miRWalk and PicTar databases, the genes regulated by miRNA-146a-5p which is related to dry eye disease were selected by draw Venn diagram;Results: The comparison of the general information between patients and healthy people was no significant difference, and it indicated that the two groups were comparable. The results of databases showed that IRAK1 was one of the target genes regulated by miRNA-146a-5p and it is related to dry eye disease. The expression of miRNA-146a-5p was negatively related to IRAK1 mRNA and protein. While IRAK1 had positive correlation with IL-6, TNF-α and CBP proteins. Conclusion: These results emphasized that miRNA-146a-5p could inhibit the expression of IRAK1, IL-6, TNF-α and CBP so as to help reduce the inflammatory response in dry eye syndrome.

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MicroRNA in Sjögren’s Syndrome: Their Potential Roles in Pathogenesis and Diagnosis

Cited by 48 publications

References 80 publications

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

Distinct microRNA expression profiles in saliva and salivary gland tissue differentiate patients with primary Sjögren's syndrome from non‐Sjögren's sicca patients

The expression of miRNA-146a-5p and its mechanism of treating dry eye syndrome

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