MicroRNA in skin diseases

Рукша, Т. Г.; Komina, A. V.; Palkina, Nadezhda

doi:10.1684/ejd.2017.3024

Cited by 30 publications

(30 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Serum microRNA-7 concentration was inversely correlated with disease duration and mLoSSI (Localized Scleroderma Skin Activity Index), but not significant [9]. Knocking down of microRNA-7 in normal fibroblasts caused overexpression of COL1A2 which was demonstrated to be a direct target of miR-7 [11,25]. Therefore, miR-7 may carry in pathogenesis of localized scleroderma due to overexpression of type II collagen.…”

Section: Microrna-7mentioning

confidence: 94%

“…MicroRNAs belong to small, noncoding RNAs that represent crucial regulators of cell differentiation, proliferation, apoptosis and immune response [25,28]. Thus far, according to miRBase, 1917 microRNAs encoding sequences have been determined in the human genome.…”

Section: Micrornamentioning

confidence: 99%

“…Nonetheless, there was no correlation between serum levels of miRNA-196a and types of LoSc, clinical manifestations or laboratory tests. In cultured dermal fibroblasts, knockdown of miRNA-196 was normalized by depletion of TGF-β level [18,25]. Inhibition of microRNA-196a enhanced type I collagen synthesis in normal fibroblast, while transfection of the microRNA-196 mimic lead to downregulation of collagen in scleroderma fibroblast [11,18,25].…”

Section: Mirna-196amentioning

confidence: 99%

“…In cultured dermal fibroblasts, knockdown of miRNA-196 was normalized by depletion of TGF-β level [18,25]. Inhibition of microRNA-196a enhanced type I collagen synthesis in normal fibroblast, while transfection of the microRNA-196 mimic lead to downregulation of collagen in scleroderma fibroblast [11,18,25]. Two years earlier, the same authors reported that in systemic scleroderma there was no prominent variation between healthy controls and patient group [10].…”

Section: Mirna-196amentioning

confidence: 99%

See 3 more Smart Citations

MicroRNA in localized scleroderma: a review of literature

2019

View full text Add to dashboard Cite

show abstract

Section: Microrna-7mentioning

confidence: 94%

Section: Micrornamentioning

confidence: 99%

Section: Mirna-196amentioning

confidence: 99%

Section: Mirna-196amentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA in localized scleroderma: a review of literature

2019

View full text Add to dashboard Cite

show abstract

“…miRNAs contribute to the cellular regulatory processes via inhibiting their specific mRNA target expression [11]. It is known that miRNAs can regulate a variety of the cellular processes, including cell proliferation, apoptosis, differentiation and immune responses [12,13]. miRNAs dysregulation is associated with a variety of physiological and developmental cellular processes in human, including melanocytes and immune cells [14].…”

Section: Introductionmentioning

confidence: 99%

MiR-217 inhibition relieves oxidative stress-induced melanocyte damage by targeting sirtuin 1

Huang

Sun

2020

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Vitiligo is caused by the disappearance of melanocyte function in the skin, but the mechanism has not been fully elucidated. This study aimed to investigate the expression and role of miR-217 in dysfunctional human primary melanocytes caused by oxidative stress, so as to explore its potential role in vitiligo. Hydrogen peroxide (H 2 O 2 ) was used to induce the oxidative damage of melanocytes. The levels of sirtuin 1 (SIRT1) and miR-217 were measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and or Western blot assay. TargetScan and dual luciferase reporter gene assay were used to confirm the relationship between SIRT1 and miR-217. Cell viability and cell apoptosis were analysed by 3-(4,5-dimethyl-2-thiazolyl)À2,5diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometry assay, respectively. Reactive oxygen species (ROS) production, superoxide dismutase (SOD) and catalase (CAT) activities were determined using specific assay kits. SIRT1 was down-regulated, while miR-217 was up-regulated in H 2 O 2 -induced human primary melanocytes. SIRT1 was a target gene of miR-217. Inhibition of miR-217 increased cell viability and inhibited cell apoptosis in H 2 O 2 -treated melanocytes. Besides, inhibition of miR-217 enhanced the antioxidant activity of SOD and CAT and reduced the accumulation of intracellular ROS. Notably, all these effects were reversed by SIRT1-siRNA. The data indicated that SIRT1 was down-regulated, while miR-217 was up-regulated in dysfunctional human primary melanocytes caused by oxidative stress, and miR-217 inhibition relieved oxidative stress-induced melanocyte damage via targeting SIRT1, indicating that miR-217 might a potential target for vitiligo treatment. ARTICLE HISTORY

show abstract

Diagnosis value of miR‐181, miR‐652, and CA72‐4 for gastric cancer

Tian

Pang

Luan

2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Purpose To find a useful disease marker for early diagnosis of gastric cancer, we tried to explore the expression of serum miR‐181, miR‐652, and carbohydrate antigen 72‐4 (CA72‐4). Patients and Methods According to clinical pathologic stages, 112 patients with gastric cancer were divided into early gastric cancer group ( n = 60) and advanced gastric cancer group ( n = 52), stage I‐II ( n = 65), and stage III‐IV ( n = 47). Another 50 cases of gastric benign lesions and 40 healthy controls were also selected. Real‐time quantitative PCR together with chemiluminescence were applied to detect expression levels. ROC curve was applied to judge their diagnostic efficiency. Pearson's correlation analysis was put into use to investigate the relevance of three indicators. Results Compared with benign lesions group and control group, significantly higher expression levels were found in patients of gastric cancer (all p < 0.001). Similarly, compared with early gastric cancer group, significantly higher expression levels were found in advanced gastric cancer group (all p < 0.001). The same result was also found in stage III‐IV (all p < 0.001). The best cutoff values were 0.93, 2.38, and 16.94 U/ml, respectively. The area under the curve (0.917, 95%CI: 0.856–0.975) of the three combined diagnosis of early gastric cancer was the largest, and its sensitivity and specificity were 92.5% and 86.8%. And miR‐181 and miR‐652 were positively correlated with CA72‐4 ( r = 0.772, p < 0.001, r = 0.853, p < 0.001). Conclusion Serum miR‐181, miR‐652, and CA72‐4 are closely linked to the occurrence and development of gastric cancer. Combination of three indicators has diagnostic value for early gastric cancer.

show abstract

MicroRNA in skin diseases

Cited by 30 publications

References 101 publications

MicroRNA in localized scleroderma: a review of literature

MicroRNA in localized scleroderma: a review of literature

MiR-217 inhibition relieves oxidative stress-induced melanocyte damage by targeting sirtuin 1

Diagnosis value of miR‐181, miR‐652, and CA72‐4 for gastric cancer

Contact Info

Product

Resources

About