MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella

Xu, Xiaoyan; Shen, Yubang; Fu, Jianjun; Yu, Hongyan; Huang, Wei‐Ping; Lu, Liqun; Li, Jiale

doi:10.1038/srep18595

Cited by 24 publications

(20 citation statements)

References 47 publications

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“…miR-21 also suppressed the excessive immune response and maintained body homeostasis in miiuy croaker by directly targeting TLR28. The activation of TLR5 could modulate the expression of genes involved in innate immune responses ( 71 ). cid-miRn-115 and miR-142a-3p were differentially expressed between susceptible and resistant grass carps infected with Aeromonas hydrophila .…”

Section: The Functional Role Of Mirnas In Fish Immune Regulationmentioning

confidence: 99%

Non-coding RNAs Function as Immune Regulators in Teleost Fish

Wang

Jiang

et al. 2018

Front. Immunol.

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Non-coding RNAs (ncRNAs) are functional RNA molecules that are transcribed from DNA but not translated into proteins. ncRNAs function as key regulators of gene expression and chromatin modification. Recently, the functional role of ncRNAs in teleost fish has been extensively studied. Teleost fish are a highly diverse group among the vertebrate lineage. Fish are also important in terms of aquatic ecosystem, food production and human life, being the source of animal proteins worldwide and models of biomedical research. However, teleost fish always suffer from the invasion of infectious pathogens including viruses and bacteria, which has resulted in a tremendous economic loss to the fishing industry worldwide. Emerging evidence suggests that ncRNAs, especially miRNAs and lncRNAs, may serve as important regulators in cytokine and chemokine signaling, antigen presentation, complement and coagulation cascades, and T cell response in teleost fish. In this review, we summarize current knowledge and understanding of the roles of both miRNAs and lncRNAs in immune regulation in teleost fish. Molecular mechanism insights into the function of ncRNAs in fish immune response may contribute to the development of potential biomarkers and therapeutic targets for the prevention and treatment of fish diseases.

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Section: The Functional Role Of Mirnas In Fish Immune Regulationmentioning

confidence: 99%

Non-coding RNAs Function as Immune Regulators in Teleost Fish

Wang

Jiang

et al. 2018

Front. Immunol.

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“…The let-7 family is known to regulate multiple genes related to the cell cycle and proliferation (Yang et al, 2008 ), and let-7i was shown to influence innate immunity (Chen et al, 2007 ). Recently, Xu et al ( 2016 ) studied these two carp lineages using two kidney miRNA transcriptomes from SGC or RGC infected with the highly pathogenic A. hydrophila and identified nine miRNAs differentially expressed between these groups. Furthermore, the spatial and temporal expression of a novel miRNA (cid-miRn-115) and miR-142a-3p suggests that they are potential regulators of anti-bacterial activity (Xu et al, 2016 ).…”

Section: A Review Of Mirnas In Farmed Fishesmentioning

confidence: 99%

“…Recently, Xu et al ( 2016 ) studied these two carp lineages using two kidney miRNA transcriptomes from SGC or RGC infected with the highly pathogenic A. hydrophila and identified nine miRNAs differentially expressed between these groups. Furthermore, the spatial and temporal expression of a novel miRNA (cid-miRn-115) and miR-142a-3p suggests that they are potential regulators of anti-bacterial activity (Xu et al, 2016 ). Overexpression of these miRNAs resulted in a visible change in the immune effector activity in C. idella kidney cells, and bioinformatics analysis shows that they directly regulate tlr5 expression (Xu et al, 2016 ), which is associated with the innate immune response (Yoon et al, 2012 ).…”

Section: A Review Of Mirnas In Farmed Fishesmentioning

confidence: 99%

Fishing Into the MicroRNA Transcriptome

et al. 2018

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In the last decade, several studies have been focused on revealing the microRNA (miRNA) repertoire and determining their functions in farm animals such as poultry, pigs, cattle, and fish. These small non-protein coding RNA molecules (18–25 nucleotides) are capable of controlling gene expression by binding to messenger RNA (mRNA) targets, thus interfering in the final protein output. MiRNAs have been recognized as the main regulators of biological features of economic interest, including body growth, muscle development, fat deposition, and immunology, among other highly valuable traits, in aquatic livestock. Currently, the miRNA repertoire of some farmed fish species has been identified and characterized, bringing insights about miRNA functions, and novel perspectives for improving health and productivity. In this review, we summarize the current advances in miRNA research by examining available data on Neotropical and other key species exploited by fisheries and in aquaculture worldwide and discuss how future studies on Neotropical fish could benefit from this knowledge. We also make a horizontal comparison of major results and discuss forefront strategies for miRNA manipulation in aquaculture focusing on forward-looking ideas for forthcoming research.

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“…Taken together, these results revealed that appropriate regulation of miR-146b expression is critically important for fish to resist infection. However, several reported immune-related miRNAs were not differentially expressed in the three libraries, including let-7 family, miR-155 and miR-192 [58,59,60], probably due to species- or tissue-specific miRNA expression [7,10]. …”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Cui

Wei

et al. 2016

IJMS

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MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.

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MicroRNA-induced negative regulation of TLR-5 in grass carp, Ctenopharyngodon idella

Cited by 24 publications

References 47 publications

Non-coding RNAs Function as Immune Regulators in Teleost Fish

Non-coding RNAs Function as Immune Regulators in Teleost Fish

Fishing Into the MicroRNA Transcriptome

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

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