MicroRNA let-7f is down-regulated in patients with refractory acute myeloid leukemia and is involved in chemotherapy resistance of adriamycin-resistant leukemic cells

Dai, Chong; Bai, Qing Wei; Zhang, Guang Sen; Yi, Cao; Shen, Jian; Pei, Min Fei; Yin, C. Cameron

doi:10.3109/10428194.2013.847936

Cited by 14 publications

(21 citation statements)

References 13 publications

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“…miRNA also plays an important role in tumorigenesis. In recent years, more and more studies have reported abnormal miRNA expression in drug-resistant cell lines such as cancers of the breast [7], ovary [8], and lung [9], as well as leukemia [10]. Through directly regulating key target genes, miRNAs can cause phenotypic alterations in tumor cells, thereby mediating tumor resistance to chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that transcription disorders, mutations, DNA replication anomalies, and a faulty miRNA biogenesis pathway might be the main reasons for tumor miRNA disorders [10]. For example, the enzyme Dicer, which activates miRNA, and the Argonaute2 protein were significantly downregulated in the Adriamycin-resistant breast cancer cell line MCF-7/DOX.…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of miR-33a-5p in Hepatocellular Carcinoma: A Possible Mechanism for Chemotherapy Resistance

et al. 2017

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BackgroundMulti-drug resistance is one of the major problems limiting the efficacy of cisplatin (CDDP) in treatment of hepatocellular carcinoma (HCC), and abnormal microRNA (miRNA) expression in drug-resistant cell lines plays an important role in liver cancer chemotherapy resistance.Material/MethodsWe established stable Hep3B and 97L HCC cell strains resistant to CDDP, both in vitro and in vivo. A combination of microRNA microarray and RT-qPCR experiments were used to screen differentially expressed miRNAs in HCC cell strains. A CCK-8 assay was carried out to detect and calculate the survival rates and relative inhibitory rates. Oligonucleotide transfection was used to confirm the regulatory function of the miRNA in HCC drug resistance.ResultsThe IC50 of Hep3B/CDDP(v), 97L/CDDP(v), Hep3B/CDDP(s), and 97L/CDDP(s) were significantly higher than that of their parental cells. Moreover, the doubling time of drug-resistant cells increased compared with their parent cells. MiRCURYTM LNA Array (v 16.0) high-throughput tests of resistant cell models and their parent cells showed that there were 5 downregulated microRNAs in the 4 drug-resistant cell lines, and we chose hsa-miR-33a-5p as our target for further study. Oligonucleotide transfection showed that miR-33a-5p overexpression increased the cisplatin sensitivity of Hep3B/CDDP(v) and 97L/CDDP(v) drug-resistant cells and reduced their resistance. Additionally, inhibition of miR-33a-5p expression reduced cisplatin sensitivity in Hep3B and 97L and increased their drug resistance.ConclusionsThis study confirmed that the most downregulated microRNA, miR-33a-5p, can mediate the cisplatin resistance of HCC cells, providing a new and feasible direction for research into combatting liver cancer chemotherapy resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Downregulation of miR-33a-5p in Hepatocellular Carcinoma: A Possible Mechanism for Chemotherapy Resistance

et al. 2017

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“…Besides, miR-224 expression correlated with AML subtypes, in particular the t(15,17) rearrangement, a molecular predictor of favorable clinical outcome [151]. There were additional miRNAs implicated as chemoresistance biomarkers reported in hematological malignancies [152][153][154][155][156][157][158][159][160][161][162].…”

Section: Translating Mirnas Into Clinical Applications In Hematologicmentioning

confidence: 99%

Drug resistance-related microRNAs in hematological malignancies: Translating basic evidence into therapeutic strategies

Xie

Jing

et al. 2015

Blood Reviews

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“…To sum up, the researchers predicted that CXCR4‐induced chemoresistance by down‐regulating let‐7a and led to transcriptional activation of MYC and BCLXL in AML cells by YY1 (Chen et al, ). Interestingly, increasing expression of let‐7f in K562 cell lines make the cell more sensitive to adriamycin (Dai et al, ). Recently, a dramatic increase of mir‐19a and mir‐19b expression has been detected in cases receiving the treatment of Vorinostat (histone deacetylase inhibitor‐HDACi).…”

Section: Clinical Significance Of Microrna In Outcome Estimation and mentioning

confidence: 99%

MicroRNA: an important regulator in acute myeloid leukemia

Chen

Bai

2017

Cell Biology International

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MicroRNAs (miRNAs) are a general class of endogenous non-coding RNAs with a length of 22 nucleotides, widely existing in diverse species and playing important roles in malignancies initiation and progression. MiRNAs are essential to many in vivo biological processes such as cell proliferation, apoptosis, immune response, and tumorigenesis. Significant progress till date has been made in understanding the roles of microRNAs in normal hematopoiesis and hematopoietic malignant diseases. In this review, we summarize the particular signatures of microRNAs in acute myeloid leukemia (AML) patients with specific karyotype and the clinical significance of microRNAs in early diagnosis and treatment. MicroRNAs hypermethylation was also proved to correlate with the pathogenesis of AML. However, the target genes and exact pathways of microRNAs participating in these processes are still unknown and more efforts need to be made in the near future.

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MicroRNA let-7f is down-regulated in patients with refractory acute myeloid leukemia and is involved in chemotherapy resistance of adriamycin-resistant leukemic cells

Abstract: Leuk Lymphoma Downloaded from informahealthcare.com by University of Otago on 07/07/15For personal use only.

Cited by 14 publications

References 13 publications

Downregulation of miR-33a-5p in Hepatocellular Carcinoma: A Possible Mechanism for Chemotherapy Resistance

Downregulation of miR-33a-5p in Hepatocellular Carcinoma: A Possible Mechanism for Chemotherapy Resistance

Drug resistance-related microRNAs in hematological malignancies: Translating basic evidence into therapeutic strategies

MicroRNA: an important regulator in acute myeloid leukemia

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