MicroRNA markers for forensic body fluid identification obtained from miRCURY™ LNA array

Luo, Xingguo; Li, Z.L.; Peng, Duo; Wang, L.; Zhang, L.; Liang, Weibo

doi:10.1016/j.fsigss.2015.10.006

Cited by 10 publications

(9 citation statements)

References 6 publications

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“…In the last decade, a lot of research has been carried out to identify body fluid origin using miRNAs profiling [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ].…”

Section: Mirna and Body Fluid Identificationmentioning

confidence: 99%

“…Microarray platforms highlighted many differentially expressed miRNAs [ 35 , 36 , 37 , 38 ] but qRT PCR is necessary to validate data [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. qRT-PCR is the common strategy for miRNA profiling determining the relative differences in expression of various miRNAs.…”

Section: Mirna and Body Fluid Identificationmentioning

confidence: 99%

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MicroRNAs: An Update of Applications in Forensic Science

et al. 2020

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MicroRNAs (miRNAs) are a class of non-coding RNAs containing 18–24 nucleotides that are involved in the regulation of many biochemical mechanisms in the human body. The level of miRNAs in body fluids and tissues increases because of altered pathophysiological mechanisms, thus they are employed as biomarkers for various diseases and conditions. In recent years, miRNAs obtained a great interest in many fields of forensic medicine given their stability and specificity. Several specific miRNAs have been studied in body fluid identification, in wound vitality in time of death determination, in drowning, in the anti-doping field, and other forensic fields. However, the major problems are (1) lack of universal protocols for diagnostic expression testing and (2) low reproducibility of independent studies. This review is an update on the application of these molecular markers in forensic biology.

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Section: Mirna and Body Fluid Identificationmentioning

confidence: 99%

Section: Mirna and Body Fluid Identificationmentioning

confidence: 99%

MicroRNAs: An Update of Applications in Forensic Science

et al. 2020

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“…Research has shown that miRNAs have the potential to identify forensically relevant body fluids, where a pair of oligonucleotides can be used to detect a specific miRNA sequence in high abundance in a body fluid. For example, the forensic literature has thus far demonstrated that miR‐891a‐5p is consistently differentially expressed in semen as opposed to other body fluids, while miR‐200b‐3p may be highly expressed in blood more than in other body fluids (). Furthermore, miRNAs let‐7 g and let‐7i are highly conserved among species and have shown similar abundance within and across multiple body fluids, allowing these miRNAs to be used as endogenous reference markers during analysis ().…”

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confidence: 99%

Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification

Lewis

Layne

Seashols‐Williams

2019

Journal of Forensic Sciences

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Molecular‐based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in the forensic analysis workflow. The purpose of this study was to evaluate miRNA detection in blood, semen, and saliva using DNA extraction methods commonly utilized for forensic casework. RT‐qPCR analysis revealed that the tested miRNAs were consistently detectable across most tested DNA extraction methods, but detection was significantly reduced compared to RNA extracts in some biological fluids. DNase treatment was not necessary to achieve miRNA‐specific results. A previously developed miRNA panel for forensic body fluid identification was evaluated using DNA extracts, and largely demonstrated concordance with results from samples deriving from RNA extracts of semen, blood, and saliva.

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“…Once matured, these markers extend between 19-25 nucleotide bases long and have long lifetime stabilities due to decreased susceptibility to environmental factors 64 . Several miRNA markers have been proposed for unique identification of body fluids including blood, saliva, and vaginal fluid [64][65][66][67][68][69] .…”

Section: Rna-based Methodsmentioning

confidence: 99%

“…Unfortunately, very few of the markers identified have been verified by multiple studies due to controversial results 65 . For instance, nine markers were identified for peripheral blood, but of these markers, only two, miR-451 and miR-16 were identified in multiple studies 14 .…”

Section: Rna-based Methodsmentioning

confidence: 99%

Advancing Forensic DNA Processing with Optical Detection and Microfluidic Technology

Jackson

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DNA forensic technology capabilities continue to increase with growing demands for automated, accurate, and rapid processing methods. Unfortunately, automation is commonly associated with complex and expensive instruments, and is not a viable solution for low-resource laboratories. In an effort to keep current DNA processing simple and cost-effective, this work utilizes microfluidic technology and optical detection for rapid screening and purification of forensically-relevant samples.Fast DNA screening methods are necessary to provide contextual clues for criminal cases, and determine which forensic samples are to be further processed. Two inexpensive optical methods are introduced for sequence-specific detection of nucleic acids using a single temperature amplification method known as loop-mediated isothermal amplification (LAMP). The first method exploits the high affinity between biotin-labeled LAMP amplicons and streptavidin beads for nucleic acid detection. When a target sequence is present, the biotin amplicons tether the streptavidin beads together, resulting in a sequence-specific bead aggregation response that is optically detectable down to single copies of DNA or RNA. A second optical detection method utilized LAMP with an embedded metal-indicator dye to colorimetrically detect fluid-specific mRNA markers for a panel of 5 body fluids. An optimized universal sample procedure allowed for the identification of any combination of the targeted body fluids in up to 23 samples simultaneously using a smart phone camera.DNA extraction is a critical step in DNA processing following DNA screening that is largely dominated by expensive biorobotic instruments. An affordable handheld centrifugal system and disposable polyethylene terephthalate (Pe) microdevices were developed for costeffective sample lysis and DNA purification. Pe is amendable to simple and rapid fabrication of a ii four-layer extraction device with on-board passive valving capability for increased fluidic control.Using an optimized extraction procedure, DNA was purified from whole blood samples and buccal swab lysates, which yielded strong short tandem repeat (STR) profiles. A lysis domain, specifically for buccal swab cuttings, was integrated with the extraction process to provide sampleto-PCR ready DNA within 30 minutes. Overall this work provides development towards costeffective and rapid DNA processing methods that are amendable to automation and beneficial to all forensic laboratories.iii

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MicroRNA markers for forensic body fluid identification obtained from miRCURY™ LNA array

Cited by 10 publications

References 6 publications

MicroRNAs: An Update of Applications in Forensic Science

MicroRNAs: An Update of Applications in Forensic Science

Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification

Advancing Forensic DNA Processing with Optical Detection and Microfluidic Technology

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