MicroRNA-mediated regulation of melanoma

Sun, Vera; Zhou, Wen; Majid, Shahana; Kashani-Sabet, M.; Dar, Altaf A.

doi:10.1111/bjd.12989

Cited by 36 publications

(22 citation statements)

References 74 publications

(236 reference statements)

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“…MiRNAs trigger mRNA degradation, stability or inhibition of translation by binding to specific target mRNA [32]. Accumulating evidence has suggested that dysregulation of miRNAs exerts an important role in MM progression by functioning as oncogenes or tumor suppressors [33]. Although many dysregulated miRNAs have been identified in MM, it is believed that there are still a large number of miRNAs uncovered and their unknown roles in MM progression need to be investigated.…”

Section: Discussionmentioning

confidence: 99%

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

Cui

Li²,

Lv³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Although microRNA-301a has been reported to function as an oncogene in many human cancers, the roles of miR-301a in malignant melanoma (MM) is unclear. The present study aims to investigate the functional roles of miR-301a in MM and its possible molecular mechanisms. Methods: Quantitative real-time PCR (qRT-PCR) assay was performed to detect the expression of miR-301a in MM tissues, and analyze its correlation with metastasis and prognosis of MM patients. In vitro, miR-301a was ectopically expressed using overexpression and knock-down strategies, and the effects of miR-301a expression on growth, apoptosis, migration, invasion and chemosensitivity of MM cells were further investigated. Furthermore, the potential and functional target gene was identified by luciferase reporter, qRT-PCR, Western blot assays. Results: We showed that the expression of miR-301a was significantly upregulated in MM tissues, and upregulation of miR-301a correlated with metastasis and poor prognosis of MM patients. Transfection of miR-301a/inhibitor significantly inhibited growth, colony formation, migration, invasion and enhanced apoptosis and chemosensitivity in MM cells, while transfection of miR-301a/mimic could induce the inverse effects on phenotypes of MM cells. Luciferase reporter, qRT-PCR and Western blot assays showed that phosphatase and tensin homolog (PTEN) was a direct and functional target of miR-301a. It was also observed that the Akt and FAK signaling pathways were involved in miR-301/PTEN-promoting MM progression. Conclusion: Taken together, our study suggests that miR-301a may be used as a potential therapeutic target in the treatment of human MM.

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Section: Discussionmentioning

confidence: 99%

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

Cui

Li²,

Lv³

et al. 2016

Cell Physiol Biochem

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“…Recently, it is emerging that also microRNA (miRNA) play an important role in melanoma biology acting as tumor suppressors or oncogenes [5,13,14]. Among these, miR-211 is almost restricted to the melanocyte lineage and is reported as the miRNA most differentially expressed between normal melanocytes and non-pigmented melanoma cell lines and primary melanomas from patients [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

miR‐211 and MITF modulation by Bcl‐2 protein in melanoma cells

Luca¹,

Pelosi²,

Trisciuoglio³

et al. 2015

Molecular Carcinogenesis

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Melanoma, the most lethal form of skin cancer, is frequently associated with alterations in several genes, among which the Bcl-2 oncogene plays an important role in progression, chemosensitivity and angiogenesis. Also microRNA (miRNA) are emerging as modulators of melanoma development and progression, and among them, miR-211, located within the melastatin-1/TRPM1 (transient receptor potential cation channel, subfamily M, member 1 protein) gene, is prevalently expressed in the melanocyte lineage and acts as oncosuppressor. Using several human melanoma cell lines and their Bcl-2 stably overexpressing derivatives, we evaluated whether there was a correlation between expression of Bcl-2 and miR-211. Western blot analysis and quantitative real-time polymerase chain reaction demonstrated reduced expression of pri-miR-211, miR-211, TRPM1, and MLANA levels, after Bcl-2 overexpression, associated with increased expression of well-known miR-211 target genes. Overexpression of mature miR-211 in Bcl-2 overexpressing cells rescued Bcl-2 ability to increase cell migration. A decreased nuclear localization of microphthalmia-associated transcription factor (MITF), a co-regulator of both miR-211 and TRPM1, and a reduced MITF recruitment at the TRPM1 and MLANA promoters were also evidenced in Bcl-2 overexpressing cells by immunofluorescence and chromatin immunoprecipitation experiments, respectively. Reduction of Bcl-2 expression by small interference RNA confirmed the ability of Bcl-2 to modulate miR-211 and TRPM1 expression. © 2015 Wiley Periodicals, Inc.

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“…In normal cells, c-kit modulates microphthalmia-associated transcription factor (MITF) and tyrosinase, which are mutated in melanoma cells. Depending on the cell stage of melanoma, it seems that miR-221 and miR-222 can have various targets (Table 1) (16)(17)(18)(19)(20).…”

Section: Cell-cycle Regulatory Overexpressed Mirnasmentioning

confidence: 99%

“…Considerably, these miRNAs perform their roles by reducing eukaryotic translation initiation factor (eIF4E) in melanoma cell lines (Table 1). MiR-145 is another moderator of cell cycle with different functions (1,20,43,44). MiR-145 inhibits cell reproduction owing to the adherence of c-myc and Erbb3; this function is prevented due to miR-145 depletion (38)(39)(40).…”

Section: Cell-cycle Regulatory Downregulated Mirnasmentioning

confidence: 99%

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Melanoma and Associated MicroRNAs

2016

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Context: Melanoma is an invasive type of skin cancer, with a rapidly increasing incidence. Therefore, new approaches are required to treat this aggressive cancer. MicroRNAs (miRNAs) are introduced as novel components in melanoma. This review study aimed to determine the relationship between melanoma and miRNAs. Evidence Acquisition: Prognostic, diagnostic, and therapeutic applications of miRNAs in skin cancer have been recently investigated from different aspects. Some of these studies on miRNAs were reviewed, and the mechanisms of some genetic modifications were determined in this study. Results: Since recommendations for miRNAs have increased in the past decades, and scientists have confirmed their great efficacy in multiple aspects of cancer treatment, different strategies have been developed considering their therapeutic effects. Therefore, further studies are required to confirm miRNA application in melanoma treatment. Conclusions: This review study included various investigations concerning miRNA traces as molecular rearrangements in melanoma. It was revealed that multiple miRNAs are efficient in molecular signaling and can be used as prognostic, diagnostic, and therapeutic biomarkers. Although the exact relationship between aberrant expression of miRNAs and cancers has been confirmed, further studies are required to introduce more thorough and accurate applications of miRNAs in melanoma.

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MicroRNA-mediated regulation of melanoma

Cited by 36 publications

References 74 publications

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

miR‐211 and MITF modulation by Bcl‐2 protein in melanoma cells

Melanoma and Associated MicroRNAs

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