MicroRNA miR-378 promotes BMP2-induced osteogenic differentiation of mesenchymal progenitor cells

Hupkes, Marlinda; Sotoca, Ana M.; Hendriks, José M.A.; Zoelen, Everardus J. van; Dechering, Koen J.

doi:10.1186/1471-2199-15-1

Cited by 70 publications

(61 citation statements)

References 49 publications

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“…The osteoblastic differentiation of PDLCs can be influenced by many factors, such as inflammatory microenvironment. Liu [7] found that the multi-differentiation potential of mesenchymal stem cells (MSCs) isolated from periodontitis-affected periodontal ligament tissue was significantly lower than that of MSCs isolated from healthy human periodontal ligament tissue. Furthermore, inflammatory cytokines can regulate osteogenesis of PDLCs through different signaling pathways, such as MAPKs and Smad signaling pathways [26].…”

Section: Discussionmentioning

confidence: 99%

“…miRNA-214 and miRNA-138) by targeting Osx [5] and Runx2 [6]; while the others (e.g. miRNA-378; miRNA-17; miRNA-21; miR-101; miRNA-146a; miR-132) can promote osteoblast differentiation via several pathways, by targeting BMP2 [7], Smurf1 [8], PLAP-1 [9], NF-κB signaling [10] or mTOR signaling pathway [11]. MiR-125b, one of the early discovered miRNAs, has been shown to inhibit osteoblast differentiation in the mouse MSC line ST2 [12], and it is a key regulatory factor of osteoblastic differentiation by directly targeting Cbfb [13] and indirectly acting on Runx2 at an early stage MSCs osteoblastic differentiation.…”

Section: Introductionmentioning

confidence: 99%

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miRNA-125b Regulates Osteogenic Differentiation of Periodontal Ligament Cells Through NKIRAS2/NF-κB Pathway

Nan¹,

Lin²,

Zhang³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Osteogenesis of periodontal ligament cells (PDLCS) is essential for alveolar bone repair. Varieties of factors have been found involved in the regulation of PDLCs osteoblast differentiation. Aim of this study was to identify microRNA as a regulator of the os-teogenic differentiation of PDLCs. Methods: The CD markers were analyzed by flow cytometry analysis. Osteoblast differentiation of PDLCs was induced by treatment with dexamethasone, β-glycerol phosphate and α-ascorbic acid. The expression of osteoblastic phenotype was evaluated after the induction by simultaneous monitoring of alkaline phosphatase activity, the expression of genes involved in osteoblastic differentiation by RT-qPCR and Western Blot, and mineralization at the same time. MicroRNA and NKIRAS2 expression was determined by RT-qPCR. Luciferase reporter assays were performed to test whether miR-125b is capable of interacting with the 3’UTR sequence of NKIRAS2. The possible signaling pathway was determined by Western Blot. Results: In this study, we found that the expression of miR-125b was down regulated during the process of ostoblast differentiation of PDLCs. When the expression of miR-125b was up regulated, the osteogenic differentiation of PDLCs was inhibited. During this process, the over-expressed miR-125b led to the activation of NF-κB. NF-κB inhibitor interacting RAS-like 2 (NKIRAS2) is one of target gene of miR-125b, and it is a regulator of NF-κB signaling that plays various roles in osteoblastic differentiation. We demonstrate thatmiR-125b is involved in osteogenic differentiation of PDLCs. Conclusion: Our data support the hypothesis that that miR-125b attenuates PDLCs osteoblastic differentiation by targeting NKIRAS2 and enhancing NF-κB signaling.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miRNA-125b Regulates Osteogenic Differentiation of Periodontal Ligament Cells Through NKIRAS2/NF-κB Pathway

Nan¹,

Lin²,

Zhang³

et al. 2018

Cell Physiol Biochem

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“…39 Another miRNA, miR-378, affects cell survival, tumor growth, angiogenesis, and cell differentiation. 7 Specifically, miR-378 can enhance tumor cell survival by repressing SuFu and Fus-1, 24 increase the transcriptional activity of MyoD in part by repressing MyoR, 13 promote BMP2-induced osteogenic differentiation, 17 and also regulate osteoblast differentiation by targeting GalNT-7 in MC3T3-E1 cells. 21 Previous studies also demonstrated that miR-378 suppressed cell migration and promoted cell apoptosis in prostate cancer 8 and increased the size of lipid droplets and the incorporation of acetate into triacylglycerol.…”

Section: Introductionmentioning

confidence: 99%

miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

Wei

Zhang

et al. 2016

RNA Biology

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Muscle development, or myogenesis, is a highly regulated, complex process. A subset of microRNAs (miRNAs) have been identified as critical regulators of myogenesis. Recently, miR-378a was found to be involved in myogenesis, but the mechanism of how miR-378a regulates the proliferation and differentiation of myoblasts has not been determined. We found that miR-378a-3p expression in muscle was significantly higher than in other tissues, suggesting an important effect on muscle development. Overexpression of miR-378a-3p increased the expression of MyoD and MHC in C2C12 myoblasts both at the level of mRNA and protein, confirming that miR-378a-3p promoted muscle cell differentiation. The forced expression of miR-378a-3p promoted apoptosis of C2C12 cells as evidenced by CCK-8 assay and Annexin V-FITC/PI staining results. Through TargetScan, histone acetylation enzyme 4 (HDAC4) was identified as a potential target of miR-378a-3p. We confirmed targeting of HDAC4 by miR-378a-3p using a dual luciferase assay and western blotting. Our RNAi analysis results also showed that HDAC4 significantly promoted differentiation of C2C12 cells and inhibited cell survival through Bcl-2. Therefore, we conclude that miR-378a-3p regulates skeletal muscle growth and promotes the differentiation of myoblasts through the post-transcriptional down-regulation of HDAC4.

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“…Thirteen candidate RGs were analyzed under thirteen different developmental stages, seven different normal tissues and three challenged tissues with LPS. Besides six traditional RGs in amphioxus, the other seven candidate RGs were cyclophilin ( CYC )22, glucose 6-phosphate dehydrogenase ( G6PDH )23, hypoxanthine-guanine phosphoribosyltransferase ( HPRT )24, ribosomal protein L3 ( L3 )25, ribosomal protein L13 ( L13 )26, ribosomal protein S20 ( S20 )15 and TATA box binding protein ( TBP )27. Expression stability of RGs in qRT-PCR was evaluated with five algorithms (geNorm28, NormFinder29, BestKeeper30, deltaCt method31 and RefFinder32).…”

mentioning

confidence: 99%

Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus

Zhang

Zhu

Liao

et al. 2016

Sci Rep

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Amphioxus is a closest living proxy to the ancestor of cephalochordates with vertebrates, and key animal for novel understanding in the evolutionary origin of vertebrate body plan, genome, tissues and immune system. Reliable analyses using quantitative real-time PCR (qRT-PCR) for answering these scientific questions is heavily dependent on reliable reference genes (RGs). In this study, we evaluated stability of thirteen candidate RGs in qRT-PCR for different developmental stages and tissues of amphioxus by four independent (geNorm, NormFinder, BestKeeper and deltaCt) and one comparative algorithms (RefFinder). The results showed that the top two stable RGs were the following: (1) S20 and 18 S in thirteen developmental stages, (2) EF1A and ACT in seven normal tissues, (3) S20 and L13 in both intestine and hepatic caecum challenged with lipopolysaccharide (LPS), and (4) S20 and EF1A in gill challenged with LPS. The expression profiles of two target genes (EYA and HHEX) in thirteen developmental stages were used to confirm the reliability of chosen RGs. This study identified optimal RGs that can be used to accurately measure gene expression under these conditions, which will benefit evolutionary and functional genomics studies in amphioxus.

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MicroRNA miR-378 promotes BMP2-induced osteogenic differentiation of mesenchymal progenitor cells

Cited by 70 publications

References 49 publications

miRNA-125b Regulates Osteogenic Differentiation of Periodontal Ligament Cells Through NKIRAS2/NF-κB Pathway

miRNA-125b Regulates Osteogenic Differentiation of Periodontal Ligament Cells Through NKIRAS2/NF-κB Pathway

miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development

Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus

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