MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3

Xu, Zefeng; Chen, Zeming; Peng, Min-Si; Zhang, Zhuliang; Luo, Weixiang; Shi, Rui-Yue; Wang, Lisheng; Yun, Hong Shik

doi:10.1080/21655979.2021.2024653

Cited by 11 publications

(9 citation statements)

References 39 publications

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“…Likewise, miR‐490‐5p was also downregulated in renal cell cancer, and its restoration repressed cell proliferative, migration and invasive capacities 19 . Recent studies have also shown reduced expression of miR‐490‐5p in various cancers, including colon cancer, pancreatic cancer, and supraglottic laryngeal squamous cell carcinoma 28–30 . Consistently, our data demonstrated the downregulation of miR‐490‐5p in NSCLC, especially in PTX‐resistant NSCLC samples.…”

Section: Discussionsupporting

confidence: 86%

“…19 Recent studies have also shown reduced expression of miR-490-5p in various cancers, including colon cancer, pancreatic cancer, and supraglottic laryngeal squamous cell carcinoma. [28][29][30] Consistently, our data demonstrated the downregulation of miR-490-5p in NSCLC, especially in PTXresistant NSCLC samples. MiR-490-5p inhibition aggravated the migration, growth, and survival of PTX-treated NSCLC cells, suggesting that miR-490-5p deficiency conferred NSCLC PTX-resistance.…”

Section: Discussionsupporting

confidence: 80%

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Hsa_circ_0092887 targeting miR‐490‐5p/UBE2T promotes paclitaxel resistance in non‐small cell lung cancer

Wang

Zhang

Tian

2022

Clinical Laboratory Analysis

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Background Chemoresistance is a major contributing factor to cancer treatment failure. Emerging research reveals that circular RNA (circRNA) dysregulation is implicated in chemoresistance. Our current study aimed to investigate the involvement of hsa_circ_0092887 in paclitaxel (PTX) resistance in non‐small cell lung cancer (NSCLC). Methods RT‐qPCR as well as western blotting were used for the analysis of hsa_circ_0092887, miR‐490‐5p and UBE2T expression in PTX‐resistant NSCLC tumor tissues and cells. CCK‐8 assay was done to determine the IC50 value of PTX. CCK‐8 assay, wound healing assay, analysis of apoptosis related proteins (Bax and Bcl‐2), and xenograft mouse models were utilized to investigate the role of hsa_circ_0092887 in PTX‐resistance in NSCLC. The binding sites of miR‐490‐5p to hsa_circ_0092887 or UBE2T were predicted by bioinformatics tools and were verified by RIP and dual‐luciferase assays. Results Expression of hsa_Circ_0092887 was upregulated in NSCLC tumor samples/cell lines, and its expression was also higher in PTX‐resistant tumor samples/cell lines when compared with their respective controls. Silencing of hsa_circ_0092887 in PTX‐treated NSCLC cells inhibited cell proliferation and migration, induced apoptosis, and suppressed tumor growth in xenograft mouse models in vivo. MiR‐490‐5p was a direct target of hsa_circ_0092887, and UBE2T was a functional downstream target of hsa_circ_0092887/miR‐490‐5p axis. Hsa_circ_0092887 depletion‐induced anti‐cancer effects in PTX‐treated NSCLC cells were reversed by miR‐490‐5p inhibitor. Furthermore, inhibition of miR‐490‐5p strengthened UBE2T expression, thereby attenuating the anti‐cancer effects caused by UBE2T knockdown. Conclusion Hsa_circ_0092887 depletion alleviated PTX‐resistance in NSCLC cells via modulating the miR‐490‐5p/UBE2T axis, and the targeted management of hsa_circ_0092887‐mediated signaling axis might contribute to PTX‐resistance intervention in NSCLC.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 80%

Hsa_circ_0092887 targeting miR‐490‐5p/UBE2T promotes paclitaxel resistance in non‐small cell lung cancer

Wang

Zhang

Tian

2022

Clinical Laboratory Analysis

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“…The membrane-associated guanylate kinase 2 antisense RNA 3 (MAGI2-AS3) was recently identified in NPC, driving cell proliferation, migration, and EMT through the miR-218-5p/GDPD5/SEC61A1 axis, conferring cisplatin resistance in cell lines ( 124 ). The same lncRNA expression was detected by quantitative real-time PCR in pancreatic cancer cells, and its upregulation promoted EMT through the regulation of miR-490-5p ( 211 ). Moreover, MAGI2-AS1 was identified to be EMT-related and highly co-expressed with ZEB1/2 in both gastric tissues and normal stomach tissue ( 212 ).…”

Section: Lncrnas In Drug Resistance Mechanismsmentioning

confidence: 93%

Emerging role of lncRNAs in drug resistance mechanisms in head and neck squamous cell carcinoma

et al. 2022

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Head and neck squamous cell carcinoma (HNSCC) originates in the squamous cell lining the mucosal surfaces of the head and neck region, including the oral cavity, nasopharynx, tonsils, oropharynx, larynx, and hypopharynx. The heterogeneity, anatomical, and functional characteristics of the patient make the HNSCC a complex and difficult-to-treat disease, leading to a poor survival rate and a decreased quality of life due to the loss of important physiologic functions and aggressive surgical injury. Alteration of driver-oncogenic and tumor-suppressing lncRNAs has recently been recently in HNSCC to obtain possible biomarkers for diagnostic, prognostic, and therapeutic approaches. This review provides current knowledge about the implication of lncRNAs in drug resistance mechanisms in HNSCC. Chemotherapy resistance is a major therapeutic challenge in HNSCC in which lncRNAs are implicated. Lately, it has been shown that lncRNAs involved in autophagy induced by chemotherapy and epithelial–mesenchymal transition (EMT) can act as mechanisms of resistance to anticancer drugs. Conversely, lncRNAs involved in mesenchymal–epithelial transition (MET) are related to chemosensitivity and inhibition of invasiveness of drug-resistant cells. In this regard, long non-coding RNAs (lncRNAs) play a pivotal role in both processes and are important for cancer detection, progression, diagnosis, therapy response, and prognostic values. As the involvement of more lncRNAs is elucidated in chemoresistance mechanisms, an improvement in diagnostic and prognostic tools could promote an advance in targeted and specific therapies in precision oncology.

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“…While the inhibitory effects of miR-490-5p on cancer progression have been reported in various cancer types, [18][19][20][21] its specific impact…”

Section: Mir-490-5p Inhibits Os Cell Migration and Invasionmentioning

confidence: 99%

CircPVT1 promotes migration and invasion by regulating miR‐490‐5p/HAVCR2 axis in osteosarcoma cells

Zhou,

Balmer,

Song

et al. 2024

J Cellular Molecular Medi

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Circular RNAs (circRNAs) play an important role in the progression of osteosarcoma. However, the precise function of circPVT1 in osteosarcoma remains elusive. This study aims to explore the molecular mechanism underlying the involvement of circPVT1 in osteosarcoma cells. We quantified circPVT1 expression using qRT‐PCR in both control and osteosarcoma cell lines. To investigate the roles of circPVT1, miR‐490‐5p and HAVCR2 in vitro, we separately conducted overexpression and inhibition experiments for circPVT1, miR‐490‐5p and HAVCR2 in HOS and U2OS cells. Cell migration was assessed through wound healing and transwell migration assays, and invasion was measured via the Matrigel invasion assay. To elucidate the regulatory mechanism of circPVT1 in osteosarcoma, a comprehensive approach was employed, including fluorescence in situ hybridization, qRT‐PCR, Western blot, bioinformatics, dual‐luciferase reporter assay and rescue assay. CircPVT1 expression in osteosarcoma cell lines surpassed that in control cells. The depletion of circPVT1 resulted in a notable reduction in the in vitro migration and invasion of osteosarcoma cells. Mechanism experiments revealed that circPVT1 functioned as a miR‐490‐5p sequester, and directly targeted HAVCR2. Overexpression of miR‐490‐5p led to a significant attenuation of migration and invasion of osteosarcoma cells, whereas HAVCR2 overexpression had the opposite effect, promoting these abilities. Additionally, circPVT1 upregulated HAVCR2 expression via sequestering miR‐490‐5p, thereby orchestrating the migration and invasion in osteosarcoma cells. CircPVT1 orchestrates osteosarcoma migration and invasion by regulating the miR‐490‐5p/HAVCR2 axis, underscoring its potential as a promising therapeutic target for osteosarcoma.

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MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3

Cited by 11 publications

References 39 publications

Hsa_circ_0092887 targeting miR‐490‐5p/UBE2T promotes paclitaxel resistance in non‐small cell lung cancer

Hsa_circ_0092887 targeting miR‐490‐5p/UBE2T promotes paclitaxel resistance in non‐small cell lung cancer

Emerging role of lncRNAs in drug resistance mechanisms in head and neck squamous cell carcinoma

CircPVT1 promotes migration and invasion by regulating miR‐490‐5p/HAVCR2 axis in osteosarcoma cells

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