MicroRNA (miRNA) in food resources and medicinal plant

Abstract

MicroRNAs (miRNAs) are a class of 19 - 24 nucleotide long non-coding RNAs derived from hairpin precursors, regulating various biological, metabolic and developmental processes at the post-transcriptional level. Many of the known miRNAs are evolutionary conserved across diverse plant species and function in the regulatory control of fundamentally important biological processes. It is known that exogenous plant miRNAs specifically target approximately 30% of protein-coding genes in mammals. The research was focused to analyze the occurrence of selected families of miRNAs (miR156, miR168 and miR171) in less used species but nutritionally important plant food resources (flax and medlar) and medicinal plant (milk thistle). The analyses were done by two individual approaches, by (a) miRNA-based molecular markers - as a novel type of functional markers and (b) qualitative Real-Time PCR. The expression pattern of selected miRNAs was analyzed depending on various plant tissues and developmental stages. Results have confirmed the significance and reliability of novel type of markers based on miRNA molecules as well as the species-specific and tissues-specific expression patterns of plants miRNAs. Significant polymorphism profile of miR156b was detected in various flax tissues of genotypes varying in the content of alpha-linolenic acid. Conclusions indicate that the variable behavior of the miRNA molecules, depending on various factors, may reflect the variability of the gene expression regulation of the human genome. The exploitation of the background of miRNA functioning within different species and plant tissues will help us to understand the molecular machinery as well as the regulatory mechanisms involved in the expression of miRNAs in plants and consequently in human genome.

Linseed mucilage - optimized extraction procedure for genomic research and nutraceutical applications

“…In addition to the use of random RAPD primers, the amplification efficiency of isolated DNA was tested using the sequence-specific markers designed based on the sequences of short endogenous molecules with a regulatory function, microRNAs (Figure 4). Two types of miRNA-based markers have been tested (lus-miR168 and lus-miR408) whose amplification effectivity was confirmed by our previous studies (Ražná et al, 2016;Ražná et al, 2020;Ražná et al, 2021). In addition to the amplification potential of the isolated DNA from the mucilaginous matter, we were also interested to see whether the quality of PCR products could be affected by the consistency of mucilaginous matter -fresh or lyophilized.…”

MicroRNA-Based Markers in Plant Genome Response to Abiotic Stress and Their Application in Plant Genotyping

“…Genome profiling with regard to genotype origin Linum usitatissimum [30] Spatial and temporal abundance of individual miRNA markers Linum usitatissimum [33,34] Functional markers of commercial type of the crop Linum usitatissimum [35,36] Genotyping Mespilus germanica [36] Genomic authentication of varieties Silybum marianum [37] Genotyping Ginkgo biloba [38,39] Genotyping Hedera helix [40] Genotyping Persea americana -Genotyping Plantago lanceolata - Table 3.…”

The Applicability of Genetic Markers Based on Molecules Micro RNA in Agricultural Research

“…Due to the species transferability of miRNA-based primers was possible to confirmed single forward primers with each other. The effectiveness and species transferability of used primers was in previous studies [9,10,[14][15][16].…”