MicroRNA profile in the squamous cell carcinoma: prognostic and diagnostic roles

Ghafouri‐Fard, Soudeh; Gholipour, Mahdi; Taheri, Mohammad; Shirvani-Farsani, Zeinab

doi:10.1016/j.heliyon.2020.e05436

Cited by 25 publications

(17 citation statements)

References 147 publications

(127 reference statements)

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“…The eight overlapping miRNAs between HNSC and CESC groups were previously discussed as genes related to cancer. High plasma level of hsa-mir-142 were reported as a HPV-independent prognostic marker of combined radio-chemotherapy in HNSC patients [ 23 ], and its expression correlated with the inhibition of cell proliferation and chemoresistance in ovarian cancer cell lines [ 24 ]. Induced overexpression of hsa-mir-150 inhibited cell invasion and metastasis in ovarian cancer [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

miRNA Expression Signatures of Therapy Response in Squamous Cell Carcinomas

Fekete

Welker

Győrffy

2020

Cancers

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Introduction: Squamous cell carcinomas (SCC) are a major subgroup of malignant tumors with a platinum-based first-line systematic chemotherapy. miRNAs play a role in various diseases and modulate therapy response as well. The aim of this study was to identify predictive miRNAs in platinum-treated SCCs. Methods: miRNA expression data of platinum-treated head and neck (HNSC), cervical (CESC) and lung (LUSC) cancer were collected from the TCGA repositories. Treatment response was defined based on presence or absence of disease progression at 18 months. Responder and nonresponder cohorts were compared using Mann–Whitney and Receiver Operating Characteristic tests. Logistic regression was developed to establish a predictive miRNA signature. Significance was set at FDR < 5%. Results: The integrated database includes 266 SCC patient samples with platinum-based therapy and available follow-up. We uncovered 16, 103, and 9 miRNAs correlated to chemotherapy response in the CESC, HNSC, and LUSC cohorts, respectively. Eight miRNAs overlapped between the CESC and HNSC subgroups, and three miRNAs overlapped between the LUSC and HNSC subgroups. We established a logistic regression model in HNSC and CESC which included six miRNAs: hsa-miR-5586 (Exp (B): 2.94, p = 0.001), hsa-miR-632 (Exp (B): 10.75, p = 0.002), hsa-miR-2355 (Exp (B): 0.48, p = 0.004), hsa-miR-642a (Exp (B): 2.22, p = 0.01), hsa-miR-101-2 (Exp (B): 0.39, p = 0.013) and hsa-miR-6728 (Exp (B): 0.21, p = 0.016). The model using these miRNAs was able to predict chemotherapy resistance with an AUC of 0.897. Conclusions: We performed an analysis of RNA-seq data of squamous cell carcinomas samples and identified significant miRNAs correlated to the response against platinum-based therapy in cervical, head and neck, and lung tumors.

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Section: Discussionmentioning

confidence: 99%

miRNA Expression Signatures of Therapy Response in Squamous Cell Carcinomas

Fekete

Welker

Győrffy

2020

Cancers

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“…Previous studies constructed a prognostic stratification system from multiple perspectives, such as immunity-related gene-based signature, 15 microRNA-based signature, 16 , 17 and microenvironment-based system. 18 Few studies focused on EMT-related gene-based signature.…”

Section: Discussionmentioning

confidence: 99%

An Epithelial–Mesenchymal Transition Hallmark Gene-Based Risk Score System in Head and Neck Squamous-Cell Carcinoma

Liang¹,

Wang²,

Du³

et al. 2021

IJGM

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Background Epithelial-to-mesenchymal transition (EMT) program plays a critical role in cancer. Thus, we attempted to generate a risk score system according to the expression pattern of different EMT hallmark genes in head and neck squamous-cell carcinoma (HNSC). Methods Differentially expressed EMT hallmark genes were screened to generate a risk score (RS) on TCGA HNSC dataset. The relative prognostic value of the RS compared to clinicopathological characteristics was explored using multivariable Cox analysis. Functional enrichment analysis was performed to reveal the biological characteristics. An external dataset was applied to validate the prognostic value of the RS . Results Nine genes constituted the EMT hallmark gene-based RS , which is significantly associated with poor prognosis and could successfully divide patients with HNSC into high- and low-risk groups. The RS was also an independent prognostic indicator compared to routine clinical factors. Conclusion We proposed and validated a nine-EMT hallmark gene-based risk score system in HNSC.

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“…for HNSCC [20], since the chemotherapy used for metastatic disease gives often low responses [21,22].…”

Section: Chemistrymentioning

confidence: 99%

“…HNSCC are collocated at the sixth position among more diffuse malignant tumors in the world [17], being characterized by a poor prognosis, mainly due to metastases, the development of multiple primary tumors, and local and regional, often inoperable, relapses [18,19]. The presence of distant metastases at the moment of diagnosis is associated with a high mortality rate for HNSCC [20], since the chemotherapy used for metastatic disease gives often low responses [21,22].…”

Section: Introductionmentioning

confidence: 99%

Anti-Tumoral Effects of a (1H-Pyrrol-1-yl)Methyl-1H-Benzoimidazole Carbamate Ester Derivative on Head and Neck Squamous Carcinoma Cell Lines

et al. 2021

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Nocodazole is an antineoplastic agent that exerts its effects by depolymerizing microtubules. Herein we report a structural analog of nocodazole, a (1H-pyrrol-1-yl)methyl-1H-benzoimidazole carbamate ester derivative, named RDS 60. We evaluated the antineoplastic properties of RDS 60 in two human head and neck squamous cell carcinoma (HNSCC) cell lines and we found that this compound significantly inhibited replication of both HNSCC cell lines without inducing any important cytotoxic effect on human dermal fibroblasts and human keratinocytes. The treatment of HNSCC cell lines with 1 μM RDS 60 for 24 h stopped development of normal bipolar mitotic spindles and, at the same time, blocked the cell cycle in G2/M phase together with cytoplasmic accumulation of cyclin B1. Consequently, treatment with 2 μM RDS 60 for 24 h induced the activation of apoptosis in both HNSCC cell lines. Additionally, RDS 60 was able to reverse the epithelial-mesenchymal transition and to inhibit cell migration and extracellular matrix infiltration of both HNSCC cell lines. The reported results demonstrate that this compound has a potent effect in blocking cell cycle, inducing apoptosis and inhibiting cell motility and stromal invasion of HNSCC cell lines. Therefore, the ability of RDS 60 to attenuate the malignancy of tumor cells suggests its potential role as an interesting and powerful tool for new approaches in treating HNSCC.

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MicroRNA profile in the squamous cell carcinoma: prognostic and diagnostic roles

Cited by 25 publications

References 147 publications

miRNA Expression Signatures of Therapy Response in Squamous Cell Carcinomas

miRNA Expression Signatures of Therapy Response in Squamous Cell Carcinomas

An Epithelial–Mesenchymal Transition Hallmark Gene-Based Risk Score System in Head and Neck Squamous-Cell Carcinoma

Anti-Tumoral Effects of a (1H-Pyrrol-1-yl)Methyl-1H-Benzoimidazole Carbamate Ester Derivative on Head and Neck Squamous Carcinoma Cell Lines

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