MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimental<i>Echinococcus granulosus</i>infection, using a high-throughput approach

Song, Jukun; Li, Xin; Wang, Xuhai; Ban, Qian; Hui, Wenqiao; Jia, Bin

doi:10.1051/parasite/2016023

Cited by 31 publications

(31 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Sheep are highly susceptible to cystic echinococcosis as intermediate hosts. NF-κB pathway-responsive miRNAs, which are related to the inflammation process, are expressed in significantly higher proportions in CE-resistant sheep than in non-CE-resistant sheep, specifically miR-27a, miR-542-5p, miR-134-5p, miR-21-3p, miR-26b, and miR-671 [57] (Figure 2). It could therefore be concluded from aforementioned results that the differential expression of miRNAs present in CE-resistant and non-CE-resistant sheep may be key in the response of intestinal tissues to E. granulosus.…”

Section: Mirnas and Lncrnas In Host Responses To E Granulosusmentioning

confidence: 99%

“…All of the identified miRNAs in the sheep gut and in mouse macrophages, livers, and sera were collected from published literature [43,57,60]. The confirmed miRNA families were then classified according to their annotations in these articles [43,57,60]. Twenty-two common miRNA families were distinguished in intermediate hosts (including sheep and mice) during infection with Echinococcus spp.…”

Section: Common Mirna Families In the Host Model During Infection Witmentioning

confidence: 99%

“…MiRNAs (e.g., miR-181 [61], miR-30 [62], miR-365 [63], miR-378 [64], miR-449 [65], miR-99 [66], miR-130 [67], and miR-16 [68]) have multiple target genes, including mRNAs, lncRNAs, and circRNAs; this may be key in determining the common miRNAs involved in the hosts response to infection with Echinococcus spp., which exhibit different expression levels, functions, and targets in host sheep and mouse models. Many miRNAs, such as miR-181 [57], miR-21 [69], and miR-27 [70], were implemented in the regulation of the immune response in intermediate hosts that were infected with Echinococcus spp. Further research should focus on the functional mechanisms of these common miRNAs in hosts that were infected with Echinococcus spp.…”

Section: Common Mirna Families In the Host Model During Infection Witmentioning

confidence: 99%

See 2 more Smart Citations

miRNAs and lncRNAs in Echinococcus and Echinococcosis

Yan

Yuán

et al. 2020

IJMS

View full text Add to dashboard Cite

Echinococcosis are considered to be potentially lethal zoonotic diseases that cause serious damage to hosts. The metacestode of Echinococcus multilocularis and E. granulosus can result in causing the alveolar and cystic echinococcoses, respectively. Recent studies have shown that non-coding RNAs are widely expressed in Echinococcus spp. and hosts. In this review, the two main types of non-coding RNAs—long non-coding RNAs (lncRNAs) and microRNAs (miRNAs)—and the wide-scale involvement of these molecules in these parasites and their hosts were discussed. The expression pattern of miRNAs in Echinococcus spp. is species- and developmental stage-specific. Furthermore, common miRNAs were detected in three Echinococcus spp. and their intermediate hosts. Here, we primarily focus on recent insights from transcriptome studies, the expression patterns of miRNAs and lncRNAs, and miRNA-related databases and techniques that are used to investigate miRNAs in Echinococcus and echinococcosis. This review provides new avenues for screening therapeutic and diagnostic markers.

show abstract

Section: Mirnas and Lncrnas In Host Responses To E Granulosusmentioning

confidence: 99%

Section: Common Mirna Families In the Host Model During Infection Witmentioning

confidence: 99%

Section: Common Mirna Families In the Host Model During Infection Witmentioning

confidence: 99%

See 1 more Smart Citation

miRNAs and lncRNAs in Echinococcus and Echinococcosis

Yan

Yuán

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Показано, что let-7 и mir-36, mir-67, miR-71, mir-92, mir-184, mir-281, miR-307, mir-1992 и miR-3479, которые играют весомую роль во взаимоотношениях паразита и промежуточного хозяина [48,49,50,51]. Результаты, полученные Jiang et al [52] в 2016 году показали, что у овец, инвазированных E. granulosus, наблюдается гиперэкспрессия miR-21-3p, miR-134-5p, miR-542-5p и miR-671.…”

Section: роль микрорнк гельминтов взаимоотношениях паразит-хозяинunclassified

The importance of helminthic microRNAs in pathology

Pobyarjin¹,

Pashinskaya²,

Semenov³

2019

Immunopathol Allergol Infectol

View full text Add to dashboard Cite

Гельминтозы являются одними из наиболее распространенных заболеваний человека и животных. Универсальным механизмом, который используют паразиты для создания оптимальных условий существования в организме хозяина, является изменение экспрессии его генов с участием микроРНК, которые обладают мультитаргетностью. С одной стороны микроРНК может участвовать в регуляции большого количества белок-кодирующих генов, а с другой-структурный ген является мишенью для многих микроРНК. У паразитов встречаются микроРНК аналогичные синтезирующимся в организме человека. Воздействие паразитов на процессы молекулярного синтеза посредством позитивной и негативной регуляции микроРНК могут привести к нарушению пролиферации клеток и их дифференцировки с последующим запуском бластомогенных механизмов, развитием неврологических расстройств, а также служить отягчающим фактором в прогрессии инфекционных заболеваний. Дальнейшая оценка посттранскрипционных микроРНК позволит выяснить механизмы управления отношениями паразит-хозяин.

show abstract

“…E. multilocularis was also found to quantitatively modulate circulating and liver miRNAs in a mouse model of secondary (intraperitoneal) infection [39, 40]. Similarly, the closely related E. granulosus also caused changes in the intestinal miRNA transcriptome of Kazakh sheep [41]. To date, miRNA-directed therapy against helminthiases is highly appealing [42]; both parasite - and host-derived miRNAs can be targeted, which allows (i) to interfere in essential biological processes of the parasite [43–45] and (ii) to ensure that the host environment returns to a normal biological state [46].…”

Section: Introductionmentioning

confidence: 99%

Regulation of Hepatic MicroRNAs in Response to Early Stage Echinococcus multilocularis Egg Infection in C57BL/6 mice

Boubaker

Strempel

Hemphill

et al. 2019

Preprint

View full text Add to dashboard Cite

22In this study, we present a comprehensive analysis of the hepatic miRNA transcriptome in 23 mice suffering from experimental primary alveolar echinococcosis (AE), a parasitic infection 24 caused upon ingestion of Echinococcus multilocularis (E. multilocularis) eggs. At one month 25 post-infection, infected C57BL/6 mice, along with non-infected control mice, were 26 euthanized. Subsequently livers were collected and used for small RNA library preparation 27 and next-generation sequencing (NGS). The most significantly dysregulated hepatic miRNAs 28 were validated by Stem-loop RT-qPCR. We identified 28 miRNAs with significantly altered 29 expression levels upon infection with E. multilocularis. Of these, 9 were up-regulated (fold 30 change (FC) ≥ 1.5) and 19 were down-regulated (FC ≤ 0.66) as compared to the non-infected 31 controls. In infected liver tissues, mmu-miR-148a-3p and mmu-miR-101b-3p were 8-and 6-32 fold down-regulated, respectively, and the expression of mmu-miR-22-3p was reduced by 33 50%, compared to non-infected liver tissue. Conversely, significantly higher hepatic levels 34 were noted for Mus musculus (mmu)-miR-21a-5p (FC = 2.3) and mmu-miR-122-5p (FC = 35 1.8). Down-regulated miRNAs were highly enriched in Reactome and KEGG pathways of 36 angiogenesis and fatty acids biosynthesis. Moreover, relative mRNA expression levels of 37 three pro-angiogenic (VEGFA, MTOR and HIF1-α) and two lipogenic (FASN and ACSL1) 38 genes were significantly higher in livers of E. multilocularis infected mice. 39Lastly, we studied the issue related to functionally mature arm selection preference (5p and/or 40 3p) from the miRNA precursor and showed that 9 pre-miRNAs exhibited different arm 41 selection preferences in normal versus infected liver tissues. Our study provides first evidence 42 of miRNA involvement in liver pathogenesis during AE. Our future research will focus on the 43 characterization of miRNA transcriptome patterns in more advanced AE-stages towards the 44 assessment of microRNA therapy for AE, and experimentally address functional 45 characteristics of selected features presently found. 48of microRNAs (miRNAs), a class of small non-coding RNAs involved in negative regulation 49 of gene expression. Herein, we revealed that significant alterations of miRNA expression 50 occurred in murine liver subsequently to experimental infection with Echinococcus 51 multilocularis (E. multilocularis) eggs when compared to non-infected controls. 52At the early stage of murine AE, hepatic miRNAs were mainly downregulated. Respective 53 target genes of the most extensively downregulated miRNAs were involved in angiogenesis 54 and fatty acid synthesis. Indeed, angiogenic and lipogenic genes were found to be 55 significantly higher expressed in E. multilocularis infected livers relative to non-infected 56 controls. These boosted cellular pathways are advantageous for development of the E. 57 multilocularis metacestodes, since this larval stage is not able to undertake de novo fatty acid 58 synthesis, and angiogenesis ...

show abstract

MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimentalEchinococcus granulosusinfection, using a high-throughput approach

Cited by 31 publications

References 25 publications

miRNAs and lncRNAs in Echinococcus and Echinococcosis

miRNAs and lncRNAs in Echinococcus and Echinococcosis

The importance of helminthic microRNAs in pathology

Regulation of Hepatic MicroRNAs in Response to Early Stage Echinococcus multilocularis Egg Infection in C57BL/6 mice

Contact Info

Product

Resources

About