MicroRNA Profiling Using a PCR-Based Method

Ferronato, Giuliana A. de; Cerezetti, Marcela B.; Bridi, Alessandra; Prado, Cibele M.; Santos, Gislaine dos; Bastos, Natália Marins; Rosa, Paola M. S. da; Ferst, Juliana Germano; Silveira, Juliano Coelho da

doi:10.1007/978-1-0716-2823-2_11

Cited by 3 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can be used to detect not only mature microRNAs but also their precursors. For quantitative assessment of microRNA expression (both absolute and relative), polymerase chain reaction (PCR) methods can be utilized: reverse transcription PCR followed by amplification and real-time fluorescent detection [ 28 , 29 ], isothermal loop-mediated amplification [ 30 ], and rolling circle amplification [ 31 , 32 ].…”

Section: Methods For Assessing the Pool And Mirna Expressionmentioning

confidence: 99%

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

Tregub,

Ibrahimli,

Averchuk

et al. 2023

IJMS

View full text Add to dashboard Cite

In recent times, there has been a significant increase in researchers’ interest in the functions of microRNAs and the role of these molecules in the pathogenesis of many multifactorial diseases. This is related to the diagnostic and prognostic potential of microRNA expression levels as well as the prospects of using it in personalized targeted therapy. This review of the literature analyzes existing scientific data on the involvement of microRNAs in the molecular and cellular mechanisms underlying the development of pathologies such as Alzheimer’s disease, cerebral ischemia and reperfusion injury, and dysfunction of the blood–brain barrier.

show abstract

Section: Methods For Assessing the Pool And Mirna Expressionmentioning

confidence: 99%

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

Tregub,

Ibrahimli,

Averchuk

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

Transcriptomic effects of alginate hydrogel applied to the production of bovine embryos

Ferronato,

Rosa,

Bridi

et al. 2024

Heliyon

View full text Add to dashboard Cite

A Literature Review and Meta-Analysis on the Potential Use of miR-150 as a Novel Biomarker in the Detection and Progression of Multiple Sclerosis

Arcas,

Fratila,

Moga

et al. 2024

JPM

View full text Add to dashboard Cite

Background: MicroRNA-150 (miR-150) plays a critical role in immune regulation and has been implicated in autoimmune diseases like Multiple Sclerosis (MS). This review aims to evaluate miR-150’s potential as a biomarker for MS, necessitating this review to consolidate current evidence and highlight miR-150’s utility in improving diagnostic accuracy and monitoring disease progression. Methods: A comprehensive literature search was conducted in databases like PubMed, Scopus, Google Scholar, SciSpace, MDPI and Web of Science, adhering to PRISMA guidelines. Studies focusing on miR-150 implications in MS were included. Data extraction was conducted, while quality assessment was done using the NOS and AMSTAR 2 tools. With the extracted data a statistical analyses conducted. Results: 10 eligible articles were included in review. Findings show that miR-150 levels were consistently deregulated in MS patients compared to healthy controls, correlating with disease severity and clinical parameters such as (EDSS) scores and disease activity. Additionally, miR-150 is implicated in the inflammatory pathogenesis of MS, affecting immune cell regulation and inflammatory pathways. Conclusions: MiR-150 is a promising biomarker for MS, showing significant potential for improving diagnostic accuracy and monitoring disease progression. Its consistent deregulation in MS patients and correlation with clinical parameters underscore its clinical utility. Further research should validate miR-150’s salivary presence and its possible usage as a novel biomarker and therapeutic potential in the development of MS.

show abstract

MicroRNA Profiling Using a PCR-Based Method

Cited by 3 publications

References 14 publications

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

Transcriptomic effects of alginate hydrogel applied to the production of bovine embryos

A Literature Review and Meta-Analysis on the Potential Use of miR-150 as a Novel Biomarker in the Detection and Progression of Multiple Sclerosis

Contact Info

Product

Resources

About