MicroRNA regulation in mammalian adipogenesis

Romao, Josue Moura; Jin, Wencheng; Dodson, Michael V.; Hausman, Gary; Moore, S. S.; Guan, Le Luo

doi:10.1258/ebm.2011.011101

Cited by 101 publications

(90 citation statements)

References 74 publications

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“…Adipogenesis involves the differentiation of preadipocytes or mesenchymal stem cells into mature adipocytes, with the ability to assimilate lipids (Romao et al, 2011). Recent molecular-based approaches have expanded our knowledge of the impact of diet in regulating bovine adipogenesis by examining effects on mRNA (Dahlman et al, 2005;Joseph et al, 2010) or microRNA (miRNA) expression in various adipose depots (Romao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of diet on microRNA expression in ovine subcutaneous and visceral adipose tissues1

Meale

Romao

et al. 2014

Journal of Animal Science

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Knowledge of the molecular mechanisms that regulate ovine adipogenesis is very limited. MicroRNAs (miRNA) have been reported as one of the regulatory mechanisms of adipogenesis. This study aimed to compare the expression of miRNA related to ovine adipogenesis in different adipose depots and to investigate whether their expression is affected by dietary fatty acid composition. We also investigated the role of miRNA in adipogenic gene regulation. Subcutaneous and visceral adipose tissue samples were collected at slaughter from 12 Canadian Arcott lambs fed a barley-based finishing diet where an algae meal (DHA-Gold; Schizochytrium spp.) replaced flax oil and barley grain at 0 or 3% DM (n = 6). Total RNA from each tissue was subjected to quantitative real time (qRT-) PCR analysis to determine the expression of 15 selected miRNA including 11 identified from bovine adipose tissues and 4 conserved between bovine and ovine species. MicroRNAs were differentially expressed according to diet in each tissue depot (miR-142-5p and miR-376d) in visceral and miR-142-5p, miR92a, and miR-378 in subcutaneous adipose tissue; P ≤ 0.05) and in each tissue depot depending on diet (miR-101, miR-106, miR-136, miR-16b, miR-196a-1, miR-2368*, miR-2454, miR-296, miR-376d, miR-378, and miR-92a in both control and DHA-G diets and miR-478 in control; P ≤ 0.05). Six miRNA were subjected to functional analysis and 3 genes of interest (ACSL1, PPARα, and C/EBPα) were validated by qRT-PCR. Both diet and tissue depot affected expression levels of all 3 genes (P < 0.05). miR-101, miR-106, and miR-136 were negatively correlated with their respective predicted gene targets C/ EBPα, PPARα, and ACSL1 in subcutaneous adipose tissue of lambs fed DHA-G. Yet miR-142-5p and miR-101 showed no correlation with ACSL1 or C/EBPα. The variability in expression patterns of miRNA across adipose depots reflects the tissue specific nature of adipogenic regulation. Although the examined miRNA appear to be conserved across ruminant species, our results indicate the presence of ovine specific regulatory mechanisms that can be influenced by diet.

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Section: Introductionmentioning

confidence: 99%

Effect of diet on microRNA expression in ovine subcutaneous and visceral adipose tissues1

Meale

Romao

et al. 2014

Journal of Animal Science

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“…MicroRNA-712 is a potential biomarker of atherosclerosis, a cardiovascular disease associated with lipid retention and inflammation in arterial wall [52]. miRNAs play a critical role in the differentiation of stem cell progenitors into the adipocytes [53]. Decreased expression of miR-155, miR-121 and miR-122 has been observed during the adipogenic differentiation, suggesting that they act as negative regulators of differentiation.…”

Section: Role Of Mirnas In Diseasesmentioning

confidence: 99%

Recent developments in understanding the mechanism and functions of microRNAs

Muzaffar¹,

Bisht²

2017

J App Biol Biotech

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Based on their mechanism of action and biological function, several classes of small RNAs have come into the limelight in the last two decades. These small RNA molecules generally belong to three main categories: short interfering RNAs (siRNAs), microRNAs (miRNAs), and piwi-interacting RNAs (piRNAs). miRNAs and siRNAs are distinguished primarily because miRNAs are endogenous in nature and are expressed by an organism's own genome, whereas siRNAs are exogenous in origin and derived mainly from the viruses and transposons. The first miRNA, lin-4, was discovered in 1993 as an endogenous regulator of genes that control developmental timing in Caenorhabditis elegans. miRNAs are coded by both plant and animal genomes and their transcription is typically performed by RNA polymerase II. MicroRNAs repress the expression of many genes by accelerating messenger RNA degradation as well as translational inhibition, thereby reducing the level of protein. Due to their involvement in various diseases like cancer, miRNAs have been a focus of scientific research for their potential as a new generation of drugs. The recent findings in miRNA research have been summarized in this review to add new dimensions to miRNA mechanism and functions.

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“…Unique expression pattern of miR-155, miR-221, let-7 and miR-222 are known to be associated with adipogenesis and obesity. The way for possible obesity treatments on the genetic level is going to be sought in ongoing projects (Zuo et al, 2006;Romao et al, 2011;Skarn et al, 2012).…”

Section: Mirnas and Obesitymentioning

confidence: 99%

MiRNA Molecular Profiles in Human Medical Conditions: Connecting Lung Cancer and Lung Development Phenomena

Aghanoori¹,

Mirzaei²,

Tavallaei³

2014

Asian Pacific Journal of Cancer Prevention

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MiRNAs are endogenous, single stranded ~22-nucleotide non-coding RNAs (ncRNAs) which are transcribed by RNA polymerase II and mediate negative post-transcriptional gene regulation through binding to 3'untranslated regions (UTR), possibly open reading frames (ORFs) or 5'UTRs of target mRNAs. MiRNAs are involved in the normal physiology of eukaryotic cells, so dysregulation may be associated with diseases like cancer, and neurodegenerative, heart and other disorders. Among all cancers, lung cancer, with high incidence and mortality worldwide, is classified into two main groups: non-small cell lung cancer and small cell lung cancer. Recent promising studies suggest that gene expression profiles and miRNA signatures could be a useful step in a noninvasive, low-cost and repeatable screening process of lung cancer. Similarly, every stage of lung development during fetal life is associated with specific miRNAs. Since lung development and lung cancer phenomena share the same physiological, biological and molecular processes like cell proliferation, development and shared mRNA or expression regulation pathways, and according to data adopted from various studies, they may have partially shared miRNA signature. Thus, focusing on lung cancer in relation to lung development in miRNA studies might provide clues for lung cancer diagnosis and prognosis.

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MicroRNA regulation in mammalian adipogenesis

Cited by 101 publications

References 74 publications

Effect of diet on microRNA expression in ovine subcutaneous and visceral adipose tissues1

Effect of diet on microRNA expression in ovine subcutaneous and visceral adipose tissues1

Recent developments in understanding the mechanism and functions of microRNAs

MiRNA Molecular Profiles in Human Medical Conditions: Connecting Lung Cancer and Lung Development Phenomena

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