MicroRNA sequencing and molecular mechanisms analysis of the effects of gold nanoparticles on human dermal fibroblasts

Huang, Yan; Lü, Xiaoying; Qu, Yinghua; Yang, Yamin; Wu, Si Han

doi:10.1016/j.biomaterials.2014.10.042

Cited by 32 publications

(18 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the formation of pre-miRNA in the nucleus, this species is transferred into the cytoplasm through Exportin-5. Once in the cell cytoplasm, pre-miRNA species is cleaved by a second RNase III endonuclease, called Dicer, along with transactivator RNA binding protein (TRBP) to form mature miRNA (double-stranded) and miRNA ∗ (passenger strand) [ 11 ]. Eventually miRNA will be degraded by the Argonaute protein.…”

Section: Structural and Biochemical Aspects Of Mirnasmentioning

confidence: 99%

Use of miRNAs as Biomarkers in Sepsis

Dumache

Rogobete

Bedreag

et al. 2015

Analytical Cellular Pathology

View full text Add to dashboard Cite

Sepsis is one of the most common causes of death in critical patients. Severe generalized inflammation, infections, and severe physiological imbalances significantly decrease the survival rate with more than 50%. Moreover, monitoring, evaluation, and therapy management often become extremely difficult for the clinician in this type of patients. Current methods of diagnosing sepsis vary based especially on the determination of biochemical-humoral markers, such as cytokines, components of the complement, and proinflammatory and anti-inflammatory compounds. Recent studies highlight the use of new biomarkers for sepsis, namely, miRNAs. miRNAs belong to a class of small, noncoding RNAs with an approximate content of 19–23 nucleotides. Following biochemical and physiological imbalances, the expression of miRNAs in blood or other body fluids changes significantly. Moreover, its stability, specificity, and selectivity make miRNAs ideal candidates for sepsis biomarkers. In conclusion, we can affirm that stable species of circulating miRNAs represent potential biomarkers for monitoring the evolution of sepsis.

show abstract

Section: Structural and Biochemical Aspects Of Mirnasmentioning

confidence: 99%

Use of miRNAs as Biomarkers in Sepsis

Dumache

Rogobete

Bedreag

et al. 2015

Analytical Cellular Pathology

View full text Add to dashboard Cite

show abstract

“…It has been shown that several types of nanoparticles induce changes in miRNA expression [6,7,8,9,10,11,12,13,14], DNA methylation [15,16,17,18,19,20,21,22,23,24,25,26], as well as histone modifications such as acetylation [20,27], phosphorylation [28,29] and methylation [30]. Three of the most prevalent nanomaterials used in medicine and industry are silver (AgNPs), gold (AuNPs), and superparamagnetic iron oxide nanoparticles (SPIONs).…”

Section: Introductionmentioning

confidence: 99%

“…Three of the most prevalent nanomaterials used in medicine and industry are silver (AgNPs), gold (AuNPs), and superparamagnetic iron oxide nanoparticles (SPIONs). Both AgNPs and AuNPs have been shown to alter miRNA expression and induce DNA methylation [6,7,11,12,17,20,21]. Moreover, it has been shown that both types of nanoparticles alter histone H3 phosphorylation [28,29,31,32], while AgNPs also alter histone H3 methylation [30] and decrease its acetylation [20].…”

Section: Introductionmentioning

confidence: 99%

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

2019

View full text Add to dashboard Cite

The increasing use of nanoparticles (NPs) in various applications entails the need for reliable assessment of their potential toxicity for humans. Originally, studies concerning the toxicity of NPs focused on cytotoxic and genotoxic effects, but more recently, attention has been paid to epigenetic changes induced by nanoparticles. In the present research, we analysed the DNA methylation status of genes related to inflammation and apoptosis as well as the expression of miRNAs related to these processes in response to silver (AgNPs), gold (AuNPs), and superparamagnetic iron oxide nanoparticles (SPIONs) at low cytotoxic doses in HepG2 cells. There were no significant differences between treated and control cells in the DNA methylation status. We identified nine miRNAs, the expression of which was significantly altered by treatment with nanoparticles. The highest number of changes was induced by AgNPs (six miRNAs), followed by AuNPs (four miRNAs) and SPIONs (two miRNAs). Among others, AgNPs suppressed miR-34a expression, which is of particular interest since it may be responsible for the previously observed AgNPs-mediated HepG2 cells sensitisation to tumour necrosis factor (TNF). Most of the miRNAs affected by NP treatment in the present study have been previously shown to inhibit cell proliferation and tumourigenesis. However, based on the observed changes in miRNA expression we cannot draw definite conclusions regarding the pro- or anti-tumour nature of the NPs under study. Further research is needed to fully elucidate the relation between observed changes in miRNA expression and the effect of NPs observed at the cellular level. The results of the present study support the idea of including epigenetic testing during the toxicological assessment of the biological interaction of nanomaterials.

show abstract

“…Once nanoparticles were internalized in to the cells, biological process of the cells can be wildly changed. In this study, we concerned about the neurotoxicity effect of SPIONs [ 50 – 53 ].…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor

Sun

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

Though nanomaterials are considered as drug carriers or imaging reagents targeting the central nervous system their cytotoxicity effect on neuronal cells has not been well studied. In this study, we treated PC12 cells, a model neuronal cell line, with a nanomaterial that is widely accepted for medical use, superparamagnetic iron oxide nanoparticles (SPIONs). Our results suggest that, after treated with SPIONs, the expression pattern of the cellular miRNAs changed widely in PC12 cells. As potential miRNA targets, NMDAR, one of the candidate mRNAs that were selected using GO and KEGG pathway enrichment, was significantly down regulated by SPIONs treatment. We further illustrated that SPIONs may induce cell death through NMDAR suppression. This study revealed a NMDAR neurotoxic effect of SPIONs and provides a reliable approach for assessing the neurocytotoxic effects of nanomaterials based on the comprehensive annotation of miRNA profiling.

show abstract

MicroRNA sequencing and molecular mechanisms analysis of the effects of gold nanoparticles on human dermal fibroblasts

Cited by 32 publications

References 42 publications

Use of miRNAs as Biomarkers in Sepsis

Use of miRNAs as Biomarkers in Sepsis

Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells

Comprehensive Evaluation of microRNA Expression Profiling Reveals the Neural Signaling Specific Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) through N-Methyl-D-Aspartate Receptor

Contact Info

Product

Resources

About