MicroRNA signatures associated with immortalization of EBV-transformed lymphoblastoid cell lines and their clinical traits

Lee, J.-E.; Hong, Eun-Jung; Nam, Hyun-Yeol; Kim, J.-W.; Han, Bok‐Ghee; Jeon, Jae‐Pil

doi:10.1111/j.1365-2184.2010.00717.x

Cited by 26 publications

(15 citation statements)

References 37 publications

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“…APC/Wnt/β - catenin and PI3KCA ) converge on miR-135b, causing its progressive upregulation [34]. MiR-1296 was found to downregulate tumour suppressor XAF1 in immortalised lymphoblastoid cells [35]. Interestingly, miR-539, upregulated in the ‘high-risk’ early stage CC, targets O-GlcNAcase (OGA), an enzyme that posttranslationally removes O-linked β-N-acetylglucosamines residues [36].…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of 5-microRNA based signature in T2-T3N0 colon cancer

Bobowicz

Skrzypski

Czapiewski

et al. 2016

Clin Exp Metastasis

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The role of adjuvant chemotherapy in stage T2-T3N0 colon cancer (CC) is controversial and there are currently no reliable factors allowing for individual selection of patients with high risk of relapse for such therapy. We searched for microRNA-based signature with prognostic significance in this group. We assessed by qRT-PCR expression of 754 microRNAs (miRNAs) in tumour samples from 85 stage pT2-3N0 CC patients treated with surgery alone. MiRNA expression was compared between two groups of patients: 40 and 45 patients who did and did not develop distant metastases after resection, respectively. Additionally, miRNA expression was compared between CC and normal colon mucosa samples and between the mismatch repair (MMR) competent and deficient tumours. Low expression of miR-1300 and miR-939 was significantly correlated with shorter distant metastasis-free survival (DMFS) in Cox univariate analysis (p.adjusted = 0.049). The expression signature of five miRNAs (miR-1296, miR-135b, miR-539, miR-572 and miR-185) was found to be prognostic [p = 1.28E−07, HR 8.4 (95 % CI: 3.81–18.52)] for DMFS and cross-validated in a leave-one-out analysis, with the sensitivity and specificity of 74 and 78 %, respectively. The expression of miR-592 was significantly associated with the MMR status (p.adjusted <0.01). The expression of several novel miRNAs were found to be tumour specific, e.g. miR-888, miR-523, miR-18b, miR-302a, miR-423-5p, miR-582-3p (p < 0.05). We developed a miRNA expression signature that may be predictive for the risk of distant relapse in early stage CC and confirmed previously reported association between miR-592 expression and MMR status.Electronic supplementary materialThe online version of this article (doi:10.1007/s10585-016-9810-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Prognostic value of 5-microRNA based signature in T2-T3N0 colon cancer

Bobowicz

Skrzypski

Czapiewski

et al. 2016

Clin Exp Metastasis

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“…Another method to immortalize human memory B cells is by Epstein-Barr virus (EBV) mediated transformation (Lee et al, 2011;Klein, 1996;Straub and Zubler, 1989;Ohashi et al, 2017;McLaughlin et al, 2017;Traggiai et al, 2004). The C3b, EBV-binding receptor positive B cells can be immortalized to generate antibodies for isolation using single cell sorting, or can be cultured with feeder cells for further screening or cloning.…”

Section: Hybridoma Technique B Cell Immortalization and Microneutralmentioning

confidence: 99%

Brief introduction of current technologies in isolation of broadly neutralizing HIV-1 antibodies

Sun

Yan

Tang³

et al. 2018

Virus Research

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HIV/AIDS has become a worldwide pandemic. Before an effective HIV-1 vaccine eliciting broadly neutralizing monoclonal antibodies (bnmAbs) is fully developed, passive immunization for prevention and treatment of HIV-1 infection may alleviate the burden caused by the pandemic. Among HIV-1 infected individuals, about 20% of them generated cross-reactive neutralizing antibodies two to four years after infection, the details of which could provide knowledge for effective vaccine design. Recent progress in techniques for isolation of human broadly neutralizing antibodies has facilitated the study of passive immunization. The isolation and characterization of large panels of potent human broadly neutralizing antibodies has revealed new insights into the principles of antibody-mediated neutralization of HIV. In this paper, we review the current effective techniques in broadly neutralizing antibody isolation.

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“…The mechanisms underlying this regulation may involve miRNAs and/or epigenetic regulation (Byun et al, 2003;Lee et al, 2011;Zou et al, 2006).…”

Section: Sarm1 Deficiency Leads To Xaf1 Upregulation and Enhanced Apomentioning

confidence: 99%

SARM1 deficiency, which prevents Wallerian degeneration, upregulates XAF1 and accelerates prion disease

Zhu

Frontzek

et al. 2018

Preprint

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Condensed title: SARM1 deficiency accelerates prion diseasesAbbreviations: CNS, central nervous system; dpi, days post inoculation; MyD88, myeloid differentiation primary response gene 88; NBH, non-infectious brain homogenates; PK, proteinase K; qRT-PCR, quantitative real-time PCR; RML6, Rocky Mountain Laboratories scrapie strain passage 6; SARM1, sterile α and HEAT/armadillo motifs containing protein; XAF1, X-linked inhibitor of apoptosis-associated factor 1. | P a g eAbstract SARM1 (sterile α and HEAT/armadillo motifs containing protein) is a member of the MyD88 (myeloid differentiation primary response gene 88) family which mediates innate immune responses. Because inactivation of SARM1 prevents various forms of axonal degeneration, we tested whether it might protect against prion-induced neurotoxicity. Instead, we found that SARM1 deficiency exacerbates the progression of prion pathogenesis. This deleterious effect was not due to SARM1-dependent modulation of prion-induced neuroinflammation, since microglial activation, astrogliosis and brain cytokine profiles were not altered by SARM1 deficiency. Wholetranscriptome analyses indicated that SARM1 deficiency led to strong, selective overexpression of the pro-apoptotic gene XAF1 (X-linked inhibitor of apoptosis-associated factor 1). Consequently, the activity of proapoptotic caspases and neuronal death were enhanced in prion-infected SARM1 -/mice. These results point to an unexpected function of SARM1 as a regulator of prioninduced neurodegeneration, and suggest that XAF1 might constitute a therapeutic target in prion disease.

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MicroRNA signatures associated with immortalization of EBV-transformed lymphoblastoid cell lines and their clinical traits

Cited by 26 publications

References 37 publications

Prognostic value of 5-microRNA based signature in T2-T3N0 colon cancer

Prognostic value of 5-microRNA based signature in T2-T3N0 colon cancer

Brief introduction of current technologies in isolation of broadly neutralizing HIV-1 antibodies

SARM1 deficiency, which prevents Wallerian degeneration, upregulates XAF1 and accelerates prion disease

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