MicroRNA156 improves drought stress tolerance in alfalfa ( Medicago sativa ) by silencing SPL13

Arshad, Muhammad; Feyissa, Biruk A.; Amyot, Lisa; Aung, Banyar; Hannoufa, Abdelali

doi:10.1016/j.plantsci.2017.01.018

Cited by 208 publications

(205 citation statements)

References 79 publications

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“…In addition, miR156 regulates tolerance to environmental stresses such as salt and drought stresses via downregulation of the SPL (squamosa promoter-binding protein-like) transcription factor family genes. These genes are master regulators of various biological processes, including vegetative to reproductive phase change, secondary metabolism, and stress responses [33,37,38]. It has been shown that the expression of miR169 is regulated by CBF/DREB (C-repeat-binding factor/dehydration-responsive element binding factor) transcription factors [39,40].…”

Section: Drought-responsive Tomato Long Non-coding Rna Transcripts Asmentioning

confidence: 99%

Identification and Functional Prediction of Drought-Responsive Long Non-Coding RNA in Tomato

Eom

Lee²,

Lee³

et al. 2019

Agronomy

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In higher plants, several lines of evidence suggest that long non-coding RNAs (lncRNAs) may play important roles in the regulation of various biological processes by regulating gene expression. In this study, we identified a total of 521 lncRNAs, classified as intergenic, intronic, sense, and natural antisense lncRNAs, from RNA-seq data of drought-exposed tomato leaves. A further 244 drought-responsive tomato lncRNAs were predicted to be putative targets of 92 tomato miRNAs. Expression pattern and preliminary functional analysis of potential mRNA targets suggested that drought-responsive tomato lncRNAs play important roles in a variety of biological processes via lncRNA–mRNA co-expression. Taken together, these data present a comprehensive view of drought-responsive tomato lncRNAs that serve as a starting point for understanding the role of long intergenic non-coding RNAs in the regulatory mechanisms underlying drought responses in crops.

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Section: Drought-responsive Tomato Long Non-coding Rna Transcripts Asmentioning

confidence: 99%

Identification and Functional Prediction of Drought-Responsive Long Non-Coding RNA in Tomato

Eom

Lee²,

Lee³

et al. 2019

Agronomy

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“…Intriguingly, it has recently become apparent that the modulation of these epigenetic factors may, at least in some cases, translate into improved abiotic stress tolerance. For instance, the overexpression of miR169, miR408, and miR319, respectively, has been found to enhance abiotic stress tolerance in various plant species (Hajyzadeh, Turktas, Khawar, & Unver, ; Yang et al, ; Zhang et al, ; Zhou et al, ), and the overexpression of miR156 has also recently been found to improve drought and salinity stress tolerance in alfalfa (Arshad, Feyissa, Amyot, Aung, & Hannoufa, ; Arshad, Gruber, Wall, & Hannoufa, ). Although miR156's role in drought tolerance in alfalfa appears to be related to its transcript cleavage‐based silencing of the SPL13 gene (Arshad et al, ), a further unraveling of the interplay between these diverse regulatory mechanisms is going to be a necessity for gaining a full understanding of the molecular pathways driving abiotic stress response in plants, and also for the successful design of biotechnological strategies with which to improve this trait in the future.…”

Section: Introductionmentioning

confidence: 99%

Molecular improvement of alfalfa for enhanced productivity and adaptability in a changing environment

2017

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Due to an expanding world population and increased buying power, the demand for ruminant products such as meat and milk is expected to grow substantially in coming years, and high levels of forage crop production will therefore be a necessity. Unfortunately, urbanization of agricultural land, intensive agricultural practices, and climate change are all predicted to limit crop production in the future, which means that the development of forage cultivars with improved productivity and adaptability will be essential. Because alfalfa (Medicago sativa L.) is one of the most widely cultivated perennial forage crops, it has been the target of much research in this field. In this review, we discuss progress that has been made towards the improvement of productivity, abiotic stress tolerance, and nutrient-use efficiency, as well as disease and pest resistance, in alfalfa using biotechnological techniques. Furthermore, we consider possible future priorities and avenues for attaining further enhancements in this crop as a means of contributing to the realization of food security in a changing environment.

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“…Previously, our group showed that overexpression of miR156 improved multiple physiological traits and altered the transcriptome pro le of alfalfa [30]. Further investigations revealed a positive role for miR156 in abiotic stress tolerance, including drought [26,31], salinity [27] and heat [28]. In addition, transcriptomic analysis showed that miR156 affects a wide array of gene families under drought stress in alfalfa [23].…”

Section: Alfalfa Proteome Is Affected By Mir156 Under Heat Stressmentioning

confidence: 97%

“…Major transcription factors regulated by miR156 belong to Squamosa Promoter Binding Protein-Like (SPL) family [24]. Previously, we identi ed miR156 target SPL genes, and characterized their functions in alfalfa, including their role in drought and salinity responses [25,26,27]. Despite a series of miR156related studies in different plant species, there has been no reported proteome analysis on miR156OE alfalfa under heat stress.…”

Section: Introductionmentioning

confidence: 99%

Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

Arshad

Puri

Simkovich

et al. 2020

Preprint

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Background Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 ° C) for 24 hours. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log 2 FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

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MicroRNA156 improves drought stress tolerance in alfalfa ( Medicago sativa ) by silencing SPL13

Cited by 208 publications

References 79 publications

Identification and Functional Prediction of Drought-Responsive Long Non-Coding RNA in Tomato

Identification and Functional Prediction of Drought-Responsive Long Non-Coding RNA in Tomato

Molecular improvement of alfalfa for enhanced productivity and adaptability in a changing environment

Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

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