MicroRNA397b negatively regulates resistance of Malus hupehensis to Botryosphaeria dothidea by modulating MhLAC7 involved in lignin biosynthesis

Yu, Xinyi; Gong, Hongyong; Cao, Lifang; Hou, Yingjun; Qu, Shenchun

doi:10.1016/j.plantsci.2019.110390

Cited by 41 publications

(26 citation statements)

References 41 publications

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“…They play important roles in cell wall lignin biosynthesis and in maintaining plant mechanical toughness and resistance to lodging and external adverse stimulation. It was reported that overexpressing Malus hupehensis LAC7 in tobacco improved the lignin content level and enhanced the resistance against pathogens (Yu et al 2020). Double mutant lac4 lac17 in Arabidopsis showed a great reduction in lignin content (Berthet et al 2011).…”

Section: Target Genes Of Mir397mentioning

confidence: 99%

“…However, further investigation is lacking. Recently, Yu et al (2020) found that transient overexpression of miR397b in Malus hupehensis leaves triggered the increased sensitivity to Botryosphaeria dothidea owing to the reduced LAC7 and lignin content, whereas the inhibition of miR397b in leaves transformed with STTM397 had opposite effects (Yu et al 2020).…”

Section: Roles Of Mir397 In Plant-microbe Interactionsmentioning

confidence: 99%

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Plant miR397 and its functions

Huang

Zhou

Gao

et al. 2021

Functional Plant Biol.

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MicroRNAs (miRNAs) are noncoding, small RNAs of 20–24 nucleotides (nt) and function critically at the post-transcriptional level to regulate gene expression through cleaving mRNA targets or interfering with translation of the target mRNAs. They are broadly involved in many biological processes in plants. The miR397 family in plants contains several conserved members either in 21-nt or in 22-nt that mainly target the laccase (LAC) genes functioning in lignin synthesis and are involved in the development of plants under various conditions. Recent findings showed that miR397b in Arabidopsis could also target to Casein Kinase II Subunit Beta 3 (CKB3) and mediate circadian regulation and plant flowering. This review aims to summarise recent updates on miR397 and provides the available basis for understanding the functional mechanisms of miR397 in plant growth and development regulation and in response to external adverse stimulation.

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Section: Target Genes Of Mir397mentioning

confidence: 99%

Section: Roles Of Mir397 In Plant-microbe Interactionsmentioning

confidence: 99%

Plant miR397 and its functions

Huang

Zhou

Gao

et al. 2021

Functional Plant Biol.

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“…The tobacco and apple leaves were transiently expressed by Agrobacterium-mediated transformation. The tobacco transient transformation was performed by pressure injection and the apple transient transformation was performed using the vacuum method (Yu et al 2020). the reliability of the transient transformation system was demonstrated by GUS staining, qRT-PCR, and the amplification of MdWRKY75d and MdWRKY75e after transient transformation in tobacco and apple, which can be used to verify gene function.…”

Section: Transient Expression Of Mdwrky75d and Mdwrky75e In Tobacco And Applesmentioning

confidence: 99%

“…However, some orchard tree species have low genetic efficiency compared to transgenic model plants such as Arabidopsis and tobacco. Thus, transient expression is sometimes used to verify gene functions for faster and more efficient verification; for example, apple Micro-RNA397b was verified for disease resistance using transient expression (Yu et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome sequencing, data-based screening, and functional investigation of MdWRKY75d and MdWRKY75e in disease-resistant apples

Hou

Chen

et al. 2021

Journal of Plant Interactions

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Sushuai' is a disease-resistant apple variety. Transcriptome sequencing revealed differentially expressed genes mainly in the metabolism, biosynthesis of secondary metabolites, plantpathogen interactions, and plant signaling transduction pathways. The jasmonate/ethylene signaling pathway and WRKY transcription factors (especially WRKY75 genes) played important roles in improving Alternaria alternata resistance in 'Sushuai'. An investigation of the effect of biotic and abiotic stresses on the expression of MdWRKY75s revealed that MdWRKY75d and MdWRKY75e expression was upregulated by A. alternata and methyl jasmonate treatments, while their expression was downregulated by salicylic acid treatment. Transient MdWRKY75d and MdWRKY75e expression improved apple resistance to A. alternata. The analysis, prediction, and validation of the transcriptome data revealed that MdLAC7, MdRFK1, and MdWFK1 are the most likely target genes of MdWRKY75d and MdWRKY75e. Our study provides a basis for investigating mechanisms of resistance to A. alternata.

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“…Cs-miR414 and cs-miR828 are involved in tea bud dormancy (Jeyaraj et al, 2014). For pathogen stress regulations, miR397 plays a negative regulatory role in apple resistance to hepatitis B virus (Yu et al, 2020), miR396 affects the susceptibility to rice blast (Chandran et al, 2019), and miR528 increases the viral defense ability of Oryza sativa (Wu et al, 2017). In the aspect of abiotic stress regulations, miR399 and miR827 are important for the resistance to phosphorus deficiency (Hackenberg et al, 2013;Du et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification and Functional Characterization of Plant MiRNA Under Salt Stress Shed Light on Salinity Resistance Improvement Through MiRNA Manipulation in Crops

Zhang

Yang

et al. 2021

Front. Plant Sci.

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Salinity, as a major environmental stressor, limits plant growth, development, and crop yield remarkably. However, plants evolve their own defense systems in response to salt stress. Recently, microRNA (miRNA) has been broadly studied and considered to be an important regulator of the plant salt-stress response at the post-transcription level. In this review, we have summarized the recent research progress on the identification, functional characterization, and regulatory mechanism of miRNA involved in salt stress, have discussed the emerging manipulation of miRNA to improve crop salt resistance, and have provided future direction for plant miRNA study under salt stress, suggesting that the salinity resistance of crops could be improved by the manipulation of microRNA.

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MicroRNA397b negatively regulates resistance of Malus hupehensis to Botryosphaeria dothidea by modulating MhLAC7 involved in lignin biosynthesis

Cited by 41 publications

References 41 publications

Plant miR397 and its functions

Plant miR397 and its functions

Transcriptome sequencing, data-based screening, and functional investigation of MdWRKY75d and MdWRKY75e in disease-resistant apples

Identification and Functional Characterization of Plant MiRNA Under Salt Stress Shed Light on Salinity Resistance Improvement Through MiRNA Manipulation in Crops

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