MicroRNAs

Bhaskaran, Manoj; Mohan, Mahesh

doi:10.1177/0300985813502820

Cited by 461 publications

(226 citation statements)

References 204 publications

(270 reference statements)

Supporting

Mentioning

226

Contrasting

Order By: Relevance

“…9, 10 Supporting this theory, essential roles for miRNAs have been discovered in most cellular processes, including cell proliferation, development, differentiation, and apoptosis. 11–14 Moreover, aberrant miRNA expression has emerged as a hallmark of human diseases, starting with cancers but now including cardiovascular disease and inflammatory lung disease. 15–18 Most reported miRNA expression patterns in humans come from miRNA detection in cells or tissue from the target organs of disease, including the global downregulation of miRNA expression in alveolar macrophages and bronchial alveolar epithelium from smokers.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Signature of Cigarette Smoking and Evidence for a Putative Causal Role of MicroRNAs in Smoking-Related Inflammation and Target Organ Damage

Willinger¹,

Rong²,

Tanrıverdi³

et al. 2017

Circ Cardiovasc Genet

View full text Add to dashboard Cite

Background Cigarette smoking increases risk for multiple diseases. MicroRNAs (miRNAs) regulate gene expression and may play a role in smoking-induced target organ damage. We sought to describe a miRNA signature of cigarette smoking and relate it to smoking-associated clinical phenotypes, gene expression, and lung inflammatory signaling. Methods and Results Expression profiling of 283 miRNAs was conducted on whole blood-derived RNA from 5,023 Framingham Heart Study participants (54.0% women, mean age 55±13 years) using TaqMan assays and high-throughput RT-qPCR. Associations of miRNA expression with smoking status and associations of smoking-related miRNAs with inflammatory biomarkers and pulmonary function were tested with linear mixed-effects models. We identified a six-miRNA signature of smoking. Five of the six smoking-related miRNAs were associated with serum levels of C-reactive protein or interleukin-6; miR-1180 was associated with pulmonary function measures at a marginally significant level. Bioinformatic evaluation of smoking-associated genes coexpressed with the miRNA signature of cigarette smoking revealed enrichment for immune-related pathways. Smoking-associated miRNAs altered expression of select inflammatory mediators in cell culture gain-of-function assays. Conclusions We characterized a novel miRNA signature of cigarette smoking. The top miRNAs were associated with systemic inflammatory markers and reduced pulmonary function, correlated with expression of genes involved in immune function, and were sufficient to modulate inflammatory signaling. Our results highlight smoking-associated miRNAs and are consistent with the hypothesis that smoking-associated miRNAs serve as mediators of smoking-induced inflammation and target organ damage. These findings call for further mechanistic studies to explore the diagnostic and therapeutic utility of smoking-related miRNAs.

show abstract

Section: Introductionmentioning

confidence: 99%

MicroRNA Signature of Cigarette Smoking and Evidence for a Putative Causal Role of MicroRNAs in Smoking-Related Inflammation and Target Organ Damage

Willinger¹,

Rong²,

Tanrıverdi³

et al. 2017

Circ Cardiovasc Genet

View full text Add to dashboard Cite

show abstract

“…using animal models for human medicine). Although organ-and breed/species-specific miRNA signatures have been documented in various domestic and non-domestic animals, studies that have directly addressed diseases or physiological dysfunctions specific to companion, avian, and exotic animals are extremely limited (6).…”

Section: Resultsmentioning

confidence: 99%

MicroRNA function in domestic animal physiology and diseases: a promising diagnostic tool for veterinary use

Chodkowska¹,

Sadkowski²,

Ostaszewski³

2017

Medycyna Weterynaryjna

View full text Add to dashboard Cite

SummaryMicroRNAs (miRNAs) are small non-coding interfering RNA molecules capable of post-transcriptionally regulating gene expression through sequence-specific base pairing to messenger ribonucleic acid (mRNA). In recent years, hundreds of miRNAs have been identified in humans, various animals and plants. The action of miRNAs has been examined in several biological processes, including tissue morphogenesis, development, cell proliferation and differentiation, apoptosis, immunity, metabolism, and major signaling pathways. Changes in miRNA expression have also been analyzed in the context of various pathological conditions, including different kinds of inflammation, cancer, cardiovascular diseases, etc. Thanks to these investigations, several miRNAs have been identified as potential sensitive diagnostic markers that may be important in monitoring physiological and pathological processes. In human medicine, microarray and real-time PCR-based diagnostic test panels with selected disease-specific miRNAs are increasingly used to predict disease occurrence or progression. In animals, they are mainly used to diagnose canine mammary cancers and infectious diseases, as well as to monitor reproduction. Until now, only few miRNAs of domestic animals have been studied in detail. Moreover, the silencing of selected miRNAs, successfully used in human medicine in diseases related to miRNA over-expression, is also emerging as a promising tool for veterinary medicine and animal breeding.This review presents recent progress in miRNA biology in various domestic animals and shows the current state of knowledge concerning miRNAs and their potential role as a diagnostic factor in veterinary sciences.

show abstract

“…MicroRNAs (miRs) are small noncoding RNAs that play important roles in gene expression by inhibiting translation or inducing target molecules degradation 19-21. MiRs also have roles in the functions of the healthy and diseased heart, such as cardiac hypertrophy, myocardial infarction, myocardial fibrosis, cardiac arrhythmia and heart failure 22-26.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-135a Regulates Apoptosis Induced by Hydrogen Peroxide in Rat Cardiomyoblast Cells

Liu¹,

Shi²,

Diao³

et al. 2017

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Oxidative stress and apoptosis are the most important pathologic features of ischemic heart disease. Recent research has indicated that microRNAs (miRs) play an essential role in apoptosis. However, whether miRs might regulate B cell lymphoma-2 (Bcl-2) protein in apoptosis during ischemic heart disease is still unclear. The aim of this study, therefore, was to confirm the regulation of microRNA-135a (miR-135a) in oxidative stress injuries induced by hydrogen peroxide (H2O2) in rat cardiomyoblast cells H9c2. To this end, we analyzed the effects of H2O2 treatment on miR-135a expression in rat cardiomyocytes. Furthermore, we upregulated and inhibited miR-135a using mimics and inhibitors, respectively, and examined the effects on cell viability and apoptosis-related proteins. We observed that miR-135a was markedly up-regulated under H2O2 treatment in rat cardiomyoblast cells. Overexpression of miR-135a blocked the Bcl-2 protein and enhanced the apoptosis induced by H2O2, and miR-135a inhibition restored Bcl-2 protein expression. Interestingly, miR-135a inhibition did not attenuate H2O2-induced apoptosis with Bcl-2 knockdown. The results of the present study indicate that miR-135a regulates H2O2-induced apoptosis in H9c2 cells via targeting Bcl-2, and that miR-135a may be a novel therapeutic target for ischemic heart disease.

show abstract

MicroRNAs

Cited by 461 publications

References 204 publications

MicroRNA Signature of Cigarette Smoking and Evidence for a Putative Causal Role of MicroRNAs in Smoking-Related Inflammation and Target Organ Damage

MicroRNA Signature of Cigarette Smoking and Evidence for a Putative Causal Role of MicroRNAs in Smoking-Related Inflammation and Target Organ Damage

MicroRNA function in domestic animal physiology and diseases: a promising diagnostic tool for veterinary use

MicroRNA-135a Regulates Apoptosis Induced by Hydrogen Peroxide in Rat Cardiomyoblast Cells

Contact Info

Product

Resources

About