MicroRNAs and lung cancer: Biology and applications in diagnosis and prognosis

Mallick, Reema; Patnaik, Santosh K.; Yendamuri, Sai

doi:10.4103/1477-3163.67074

Cited by 37 publications

(14 citation statements)

References 86 publications

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“…These findings indicate that dysfunctions of miRNAs may be associated with NSCLC. Recent studies have identified a series of miRNAs that are deregulated in NSCLC, including miR-205 (11). It was shown that ectopic expression of miR-205 in endometrial cancer promoted tumor proliferation and invasion (12).…”

Section: Introductionmentioning

confidence: 99%

miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

2013

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Non-small cell lung cancer (NSCLC) is one of the most common causes of cancer-related mortality worldwide. microRNAs (miRNAs) play critical roles in carcinogenesis. miR-205 has been shown to be upregulated in NSCLC. In the present study, we identified the promotive effects of miR-205 on various significant biological properties of NSCLC cells, and confirmed the regulation of PTEN by miR-205. The expression of miR-205 was examined by quantitative real-time PCR both in NSCLC cell lines and tissues. The effect of miR-205 on PTEN expression was assessed in NSCLC cell lines with miR-205 mimics/inhibitor to elevate/decrease miR-205 expression. Furthermore, the roles of miR-205 in regulating the biological properties of NSCLC cells, including growth, invasion and chemoresistance, were assayed using miR-205 mimic/inhibitor-transfected cells. The 3'-untranslated region (3'-UTR) of PTEN combined with miR-205 and this was confirmed by luciferase reporter assay and western blotting. miR-205 expression was increased in NSCLC cell lines as well as in tissues. Overexpression of miR-205 promoted growth, migration and invasion, and enhanced the chemoresistance of NSCLC cells. Luciferase activity and western blotting demonstrated that miR-205 negatively regulated PTEN at a posttranscriptional level. However, miR-205 knockdown suppressed these processes in A549 cells and increased the expression of PTEN protein. Furthermore, overexpression of PTEN exhibited effects identical with those of the miR-205 inhibitor in NSCLC cells. Our results demonstrated that miR-205 is involved in the tumorigenesis of NSCLC through modulation of the PTEN signaling pathway.

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Section: Introductionmentioning

confidence: 99%

miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

2013

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“…The roles of miRNAs in NSCLC have been widely studied, and critical regulators of miRNA biogenesis, Dicer, DGCR8, and Drosha, have been reported to be disrupted in lung cancer [8,9]. miRNA microarrays studies have identified many miRNAs aberrantly expressed in lung cancer [10]. Among them, miR-16 was substantially decreased [11].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‐16 promotes growth and motility by targeting HDGF in non‐small cell lung cancer cells

et al. 2013

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Edited by Tamas DalmayKeywords: miR-16 Non-small cell lung cancer HDGF Growth Migration Invasion a b s t r a c t MicroRNAs play important roles in the development and progression of non-small cell lung cancer (NSCLC). miR-16 functions as a tumor-suppressor and is inhibited in several malignancies. Herein, we validated that miR-16 is downregulated in NSCLC tissue samples and cell lines. Ectopic expression of miR-16 significantly inhibited cell proliferation and colony formation. Moreover, miR-16 suppressed cell migration and invasion in NSCLC cells. Hepatoma-derived growth factor (HDGF) was found to be a direct target of miR-16 in NSCLC cell lines. Rescue experiments showed that the suppressive effect of miR-16 on cell proliferation, colony formation, migration, and invasion is partially mediated by inhibiting HDGF expression. This study indicates that miR-16 might be associated with NSCLC progression, and suggests an essential role for miR-16 in NSCLC.

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“…After the first two miRNAs were found to target Bcl-2, an anti-apoptotic molecule that preserve the survival of malignant B cells so that are important for the pathogenesis and progression of chronic lymphocytic leukemia [7], the potential roles of miRNAs in the cancer etiology have been widely studied and some of miRNAs were identified as oncomirs [8]. In lung cancer, it is reported that the expression profile of a number of miRNAs are disrupted [9,10]. Among them, miR-143 (also named as miR-143-3p) is substantially downregulated.…”

Section: Introductionmentioning

confidence: 99%

Targeting PKCε by miR‐143 regulates cell apoptosis in lung cancer

2013

FEBS Letters

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a b s t r a c tNon-small cell lung cancer (NSCLC) is one of the most common causes for lung cancer and cancer-related death. The imbalance between cell proliferation and apoptosis was suggested to play an important role in cancer pathogenesis and PKCe is one of the widely recognized targets. Here, we demonstrate that miR-143 is aberrantly downregulated in NSCLC tissue and negatively correlates with expression of PKCe. We show that miR-143 specifically targets the 3 0 -UTR of PKCe and regulates its expression. Treatment with miR-143 inhibitor mimics cell proliferation and apoptosis imbalance in NSCLC, while inhibition of PKCe can reverse it. Our findings suggest that targeting PKCe overexpression in NSCLC should be beneficial for lung cancer therapy.

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MicroRNAs and lung cancer: Biology and applications in diagnosis and prognosis

Cited by 37 publications

References 86 publications

miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN

Downregulation of miR‐16 promotes growth and motility by targeting HDGF in non‐small cell lung cancer cells

Targeting PKCε by miR‐143 regulates cell apoptosis in lung cancer

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