MicroRNAs as a Novel Class of Diagnostic Biomarkers in Detection of Oral Carcinoma: a Meta-Analysis Study

Lin, N.-Y.; Lin, Yazhen; Fu, Xiaodan; Wu, Chuncai; Xu, Jianping; Cui, Zhaolei; Dong, Lin

doi:10.7754/clin.lab.2015.150802

Cited by 9 publications

(16 citation statements)

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“…If body fluid miRNA similarly is chiefly composed of miRNA from shed or lysed cells or secreted tumor epithelial RNA in OSCC patients [68, 69] then it may show a similar superiority to measuring markers in surgically obtained tumor tissue [11]. Until a method for tumor microdissection that is rapid, fully automated and affordable exists, the advantages of brush and liquid biopsy are many.…”

Section: Resultsmentioning

confidence: 99%

“…Distinction between benign and malignant disease is even more difficult to achieve [8]. Work with miRNAs found the same: Classifiers designed to diagnose or provide a prognosis for oral lesions worked well when first developed, but less well when applied to external datasets [8–11]. RNA-based classifiers for other cancers have been approved for usage in the clinic [12, 13].…”

Section: Few Successes For Rna-based Diagnosis or Prognosis Of Cancermentioning

confidence: 99%

“…A third method to characterize HNSCC or oral tumors is by measurement of RNA, particularly miRNA, in body fluids [11, 30, 50–52]. While saliva is an obvious source of HNSCC RNAs [53–57], they can also be detected in serum and plasma [58–64].…”

Section: Changes In Body Fluid Mirna With Oscc Resemble Those Seen Inmentioning

confidence: 99%

“…As shown here in Table 2 and as reviewed by others, a number of miRNAs are elevated in body fluid in the presence of OSCC. These tests have shown some variability in RNA markers associated with OSCC or HNSCC but hold much potential [11, 51, 65]. Sources of error have slowed the identification of miRNA markers of OSCC and other cancers in body fluids.…”

Section: Changes In Body Fluid Mirna With Oscc Resemble Those Seen Inmentioning

confidence: 99%

“…Thus the same miRNA-based classifier for OSCC developed with brush biopsy samples may share many RNAs with those detected in body fluid. This is important as measurement of low concentration of miRNAs becomes easier, and more standardized in regard to optimized methods of measurement, selection of internal standards and data normalization [11, 65], body fluid-based miRNA quantification may be the most useful for OSCC detection as it can be done without lesion detection.…”

Section: Changes In Body Fluid Mirna With Oscc Resemble Those Seen Inmentioning

confidence: 99%

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Improving accuracy of RNA-based diagnosis and prognosis of oral cancer by using noninvasive methods

2017

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RNA-based diagnosis and prognosis of squamous cell carcinoma has been slow to come to the clinic. Improvements in RNA measurement, statistical evaluation, and sample preservation, along with increased sample numbers, have not made these methods reproducible enough to be used clinically. We propose that, in the case of squamous cell carcinoma of the oral cavity, a chief source of variability is sample dissection, which leads to variable amounts of stroma mixed in with tumor epithelium. This heterogeneity of the samples, which requires great care to avoid, makes it difficult to see changes in RNA levels specific to tumor cells. An evaluation of the data suggests that, paradoxically, brush biopsy samples of oral lesions may provide a more reproducible method than surgical acquisition of samples for miRNA measurement. The evidence also indicates that body fluid samples can show similar changes in miRNAs with oral squamous cell carcinoma (OSCC) as those seen in tumor brush biopsy samples–suggesting much of the miRNA in these samples is coming from the same source: tumor epithelium. We conclude that brush biopsy or body fluid samples may be superior to surgical samples in allowing miRNA-based diagnosis and prognosis of OSCC in that they feature a rapid method to obtain homogeneous tumor cells and/or RNA.

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Section: Resultsmentioning

confidence: 99%

Section: Few Successes For Rna-based Diagnosis or Prognosis Of Cancermentioning

confidence: 99%

Section: Changes In Body Fluid Mirna With Oscc Resemble Those Seen Inmentioning

confidence: 99%

Section: Changes In Body Fluid Mirna With Oscc Resemble Those Seen Inmentioning

confidence: 99%

Section: Changes In Body Fluid Mirna With Oscc Resemble Those Seen Inmentioning

confidence: 99%

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Improving accuracy of RNA-based diagnosis and prognosis of oral cancer by using noninvasive methods

2017

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miRNAs expression of oral squamous cell carcinoma patients

et al. 2019

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microRNA expression patterns have provided new directions in the search of biomarkers with prognostic value and even in the search of novel therapeutic targets for several neoplasms. Specifically, miRNAs profiling in oral squamous cell carcinoma (OSCC) represents a web of intrigue in the study of oral carcinogenesis. The objective of the present study was twofold: to analyze miRNA profiling and estimate related biological pathways and, to assess miRNAs profiles prognostic values in a cohort of OSCC-affected patients. The first study phase comprised case-control groups: A) 8 OSCC-affected patients and 8 healthy controls. Microarray technology (Affymetrix miRNA Array Plate 4.1) was used for miRNAs expression profile. Deregulated miRNAs were studied using Diana Tools miRPath 3.0 to associate miRNA targets with molecular pathways via Kyoto Encyclopedia of Genes and Genomes (KEGG). In a second phase, 2 miRNAs chosen for the subsequent RT-qPCR validation were studied in a second OSSC cohort (n = 8). Microarray analysis identified 80 deregulated miRNAs (35 over-expressed and 45 under-expressed). Two miRNAs (miR-497-5p and miR-4417) were chosen for further validation via RT-qPCR. Prognostic analysis did not ascertain relevant relation between miR-497-5p or miR-4417 expression and clinical or pathological parameters, except high miR-4417 in the case of nodular affectation ( P = .035) and diminished miR-497-5p radiotherapy-treated patients ( P = .05). KEGG analysis revealed that deregulated miRNAs were implicated in several biological pathways such as Proteoglycans in cancer. Our data suggest an altered miRNAs profiling in OSCC-affected patients. We have verified the altered expression of miR-497-5p and miR-4417 in OSCC samples and related the deregulated miRNAs with the ‘proteoglycans in cancer’ pathway. Further longitudinal studies with large samples are warranted to confirm the present findings.

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Association of miR-497-5p and miR-4417 Expression in Oral Squamous Cell Carcinoma and Clinical Parameters

Chamorro-Petronacci¹,

Pérez‐Sayáns²,

Padín-Iruegas³

et al. 2018

Preprint

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MicroRNAs are small non-coding RNAs that are implicated in several physiological processes such as cell development, proliferation, differentiation, apoptosis, immune response and angiogenesis. In the last couple of decades, several studies on miRNA profiling in OSCC have tried to associate miRNAs with clinical characteristics such as relapse, metastasis, and survival, however, the results have been found to vary considerably, sometimes even being contradictory. The main objective of our study was to analyse and verify miRNA expression in oral squamous cell carcinoma in a Spanish population. Second, we attempted to associate the identified deregulated miRNAs with molecular pathways. 8 Oral Squamous Cell Carcinoma patients and 8 healthy control samples were analysed by a microarray Affymetrix® miRNA 4.1 array plate and validated with 8 more cases using RT-qPCR. Deregulated miRNAs were studied using Diana Tools miRPath 3.0 to associate miRNA targets with molecular pathways. Microarray analysis identified 80 deregulated miRNAs (35 over-expressed and 45 under-expressed). Only miR-497-5p and miR-4417 maintained its deregulated expression after validation with qPCR. Among the molecular pathways in which deregulated miRNAs could be implicated, the most statistically significant pathway was ‘proteoglycans in cancer’. No relationship was found between miR-497-5p or miR-4417 expression and clinical or pathological parameters except of nodular affectation (high miR-4417 expression in patients with nodular affectation, p = 0.035) and radiotherapy (diminished miR-497-5p expression in patients who needed radiotherapy, p = 0.05). We have verified the altered expression of miR-497-5p and miR-4417 in Oral Squamous Cell Carcinoma samples and related the deregulated miRNAs with the ‘proteoglycans in cancer’ pathway.

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MicroRNAs as a Novel Class of Diagnostic Biomarkers in Detection of Oral Carcinoma: a Meta-Analysis Study

Cited by 9 publications

References 0 publications

Improving accuracy of RNA-based diagnosis and prognosis of oral cancer by using noninvasive methods

Improving accuracy of RNA-based diagnosis and prognosis of oral cancer by using noninvasive methods

miRNAs expression of oral squamous cell carcinoma patients

Association of miR-497-5p and miR-4417 Expression in Oral Squamous Cell Carcinoma and Clinical Parameters

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