MicroRNAs as clinical biomarkers?

Angelini, Timothy G.; Emanueli, Costanza

doi:10.3389/fgene.2015.00240

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…MicroRNAs have been proposed as a source of biomarkers and as indicators of exposure to pathogens [ 6 , 7 ]. MicroRNAs are small non-coding RNAs that alter the transcriptome by inhibiting translation of messenger RNA, or by degrading them [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle

et al. 2016

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The objective of this study was to identify microRNAs associated with a serum antibody response to Mycoplasma bovis in beef cattle. Serum from sixteen beef calves was collected at three points: in summer after calves were born, in fall at weaning, and in the following spring. All sera collected in the summer were ELISA-negative for anti-M. bovis. By the fall, eight animals were seropositive for IgG (positive group), while eight remained negative (negative group). By spring, all animals in both groups were seropositive. MicroRNAs were extracted from sera and sequenced on the Illumina HiSeq next-generation sequencer. A total of 1,374,697 sequences mapped to microRNAs in the bovine genome. Of these, 82% of the sequences corresponded to 27 microRNAs, each represented by a minimum of 10,000 sequences. There was a statistically significant interaction between ELISA response and season for bta-miR-24-3p (P = 0.0268). All sera collected at the initial summer had a similar number of copies of this microRNA (P = 0.773). In the fall, the positive group had an increased number of copies when compared to the negative group (P = 0.021), and this grew more significant by the following spring (P = 0.0001). There were 21 microRNAs associated (P< 0.05) with season. These microRNAs could be evaluated further as candidates to potentially improve productivity in cattle. The microRNAs bta-let-7b, bta-miR- 24-3p, bta-miR- 92a, and bta-miR-423-5p, were significatly associated with ELISA status (P< 0.05). These microRNAs have been recognized as playing a role in the host defense against bacteria in humans, mice, and dairy cattle. Further studies are needed to establish if these microRNAs could be used as diagnostic marker or indicator of exposure, or whether intervention strategies could be developed as an alternative to antibiotics for controlling disease due to M. bovis.

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Section: Introductionmentioning

confidence: 99%

Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle

et al. 2016

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“…Although circulating miRs and exosomes in general certainly show potential as a biomarker, a number of technical challenges remain to be overcome before they can be routinely utilized in the clinic [ 56 , 57 ]. Assaying miR by RT-qPCR is a more complex and time-consuming procedure than a simple ELISA assay and this may negate some of the temporal advantage over the troponin assay.…”

Section: Exosomes and Circulating Mir As Biomarkers For Cardiovasculamentioning

confidence: 99%

Exosomes in Cardiovascular Medicine

Dykes

2017

Cardiol Ther

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Exosomes are small, extracellular membrane-bound particles that mediate intercellular transport of a cytosolic cargo. Exosomal transfer of micro-RNA can modify gene expression in targeted cells. Exosome-based endocrine/paracrine signaling has been shown to be involved in a wide range of physiological processes including those associated with cardiovascular injury and disease, but remains relatively poorly understood. Exosomes offer great potential to the clinical field, with applications in both diagnostics and therapeutics. A stable, circulating form of micro-RNA exists in blood protected from endogenous nucleases. This population of micro-RNA, which includes both exosomal and non-exosomal fractions, may be isolated from blood and exploited as a novel disease biomarker with the potential to deliver increased specificity and rapid diagnosis compared to conventional biomarkers. Exosomes also offer a natural drug-delivery vehicle, providing immune evasion and specific targeting through engineering of surface-displayed ligands. Much of the cardioprotective and regenerative benefits of stem-cell grafts are now thought to derive from paracrine signaling rather than direct tissue incorporation and therefore stem cell-derived exosomes offer the potential for a convenient cell-free therapeutic option, eliminating many of the risks and variability associated with stem-cell therapy. In this review, we consider the potential applications of this emerging field to cardiovascular medicine, taking myocardial infarction as our primary example.

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“…(Siomi and Siomi 2010) MiRNAs are increasingly being studied by numerous researchers, including the potential application of miRNA-based tests to the evaluation of stresses caused by environmental chemicals and to the diagnosis of lifestyle-related diseases or cancers. (Angelini and Emanueli 2015;Kreth et al 2018;Leung and Sharp 2010;Mendell and Olson 2012;Min and Chan 2015;Olejniczak et al 2018) MiRNAs as biomarker for cancers have also been elaborated by many researchers in the past decade. (Bonci et al 2016;Hayes et al 2014;Hofbauer et al 2018;Jansson and Lund 2012;Mihelich et al 2015) Since miRNA is found in blood samples, which can be drawn routinely and minimally invasively, the utilization of miRNAs in these fields is anticipated to contribute to rapid testing.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement

Aoki

Corn

Matthews

2019

Biosensors and Bioelectronics

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We investigated sequence-specific and simultaneous microRNA (miRNA) detections by surface plasmon resonance (SPR) imaging measurements on SPR chips possessing an Au spot array modified with probe DNAs based on a miRNA-detection-selective SPR signal amplification method. MiRNAs were detected with the detection limit of the attomole level by SPR imaging measurements for different miRNA concentrations on a single chip. SPR signals were enhanced based on a combination process of sequence-specific hybridization of the miRNA to the probe DNAs, extension reaction of polyadenine (poly(A)) tails by poly(A) polymerase, binding of a ternary complex of T 30-biotin/horseradish peroxidase (HRP)-biotin/streptavidin to the poly(A) tails, and the oxidation reaction of tetramethylbenzidine (TMB) on the HRP by providing a blue precipitate on the surface. This process sequence-specifically and dramatically amplified the SPR signals. This is a simple, cost-effective, and feasible signal amplification method based on the organic compound TMB instead of metal nanoparticles.

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MicroRNAs as clinical biomarkers?

Cited by 11 publications

References 28 publications

Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle

Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle

Exosomes in Cardiovascular Medicine

MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement

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