MicroRNAs as the actors in the atherosclerosis scenario

Hajibabaie, Fatemeh; Kouhpayeh, Shirin; Mirian, Mina; Rahimmanesh, Ilnaz; Boshtam, Maryam; Sadeghian, Ladan; Gheibi, Azam; Khanahmad, Hossein; Shariati, Laleh

doi:10.1007/s13105-019-00710-7

Cited by 37 publications

(33 citation statements)

References 124 publications

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“…MicroRNAs (miRs) are small non-coding RNAs that serve an important role in the regulation of the natural immune response and inflammation (15,16), and are involved in cardiovascular diseases (17,18). miR-499, a cardiac-enriched small RNA molecule, is involved in regulating cardiac function, the pathogenesis of I/R injury and has cardioprotective effects (19,20).…”

Section: Introductionmentioning

confidence: 99%

Ischemic postconditioning attenuates the inflammatory response in ischemia/reperfusion myocardium by upregulating miR‑499 and inhibiting TLR2 activation

Zhang

Huang

et al. 2020

Mol Med Rep

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Toll-like receptor 2 (Tlr2)-mediated myocardial inflammation serves an important role in promoting myocardial ischemic/reperfusion (I/R) injury. Previous studies have shown that miR-499 is critical for cardioprotection after ischemic postconditioning (IPostC). Therefore, the present study evaluated the protective effect of IPostC on the myocardium by inhibiting TLR2, and also assessed the involvement of microRNA (miR)-499. Rat hearts were subjected to 30 min of ischemia and 2 h of reperfusion. The IPostC was 3 cycles of 30 sec of reperfusion and 30 sec of re-occlusion prior to reperfusion. In total, 90 rats were randomly divided into six groups (n=15 per group): Sham; I/R; IPostC; miR-499 negative control adeno-associated virus (AAV) vectors + IPostC; miR-499 inhibitor AAV vectors + IPostC; and miR-499 mimic AAV vectors + IPostC. It was identified that IPostC significantly decreased the I/R-induced cardiomyocyte apoptotic index (29.4±2.03% in IPostC vs. 42.64±2.27% in I/R; P<0.05) and myocardial infarct size (48.53±2.49% in IPostC vs. 66.52±3.1% in I/R; P<0.05). Moreover, these beneficial effects were accompanied by increased miR-499 expression levels (as demonstrated by reverse transcription-quantitative PCR) in the myocardial tissue and decreased TLR2, protein kinase C (PKC), interleukin (IL)-1β and IL-6 expression levels (as demonstrated by western blotting and ELISA) in the myocardium and serum. The results indicated that IPostC + miR-499 mimics significantly inhibited inflammation and the PKC signaling pathway and enhanced the anti-inflammatory and anti-apoptotic effects of IPostC. However, IPostC + miR-499 inhibitors had the opposite effect. Therefore, it was speculated that IPostC may have a miR-499-dependent cardioprotective effect. The present results suggested that miR-499 may be involved in IPostC-mediated ischemic cardioprotection, which may occur via local and systemic TLR2 inhibition, subsequent inhibition of the PKC signaling pathway and a decrease in inflammatory cytokine release, including IL-1β and IL-6. Moreover, these effects will ultimately lead to a decrease in the myocardial apoptotic index and myocardial infarct size via the induction of the anti-apoptotic protein Bcl-2, and inhibition of the pro-apoptotic protein Bax in myocardium.

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Section: Introductionmentioning

confidence: 99%

Ischemic postconditioning attenuates the inflammatory response in ischemia/reperfusion myocardium by upregulating miR‑499 and inhibiting TLR2 activation

Zhang

Huang

et al. 2020

Mol Med Rep

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“…In particular, a promising biomarker may be the level of miRNA expression, as these molecules play an important role in many cellular processes, such as proliferation, apoptosis and differentiation [ [46] , [47] , [48] ]. It is shown that miRNA take part in the development of different pathologies: barrenness, diseases of cardiovascular system, neurological illnesses and oncological diseases [ 49 , 50 ]. However, the use of miRNA as diagnostic and therapeutic biomarkers in the clinical field is a very hard task.…”

Section: Discussionmentioning

confidence: 99%

An original biomarker for the risk of developing cardiovascular diseases and their complications: Telomere length

et al. 2021

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“…miRNAs are highly conserved small non-coding RNAs that serve crucial roles in diverse physiological and pathological processes (17,18). Increasing evidence has suggested that changes in miRNA expression profiles may be associated with chronic vascular inflammation, consisting of dysfunctions in ECs, macrophages and vascular smooth muscle cells (VSMCs) (19,20).…”

Section: Genistein Alleviates Chronic Vascular Inflammatory Response mentioning

confidence: 99%

Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice

Xie

Liu

et al. 2021

Mol Med Rep

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MicroRNAs as the actors in the atherosclerosis scenario

Cited by 37 publications

References 124 publications

Ischemic postconditioning attenuates the inflammatory response in ischemia/reperfusion myocardium by upregulating miR‑499 and inhibiting TLR2 activation

Ischemic postconditioning attenuates the inflammatory response in ischemia/reperfusion myocardium by upregulating miR‑499 and inhibiting TLR2 activation

An original biomarker for the risk of developing cardiovascular diseases and their complications: Telomere length

Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice

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