microRNAs Distinctively Regulate Vascular Smooth Muscle and Endothelial Cells: Functional Implications in Angiogenesis, Atherosclerosis, and In-Stent Restenosis

Santulli, Gaetano

doi:10.1007/978-3-319-22380-3_4

Cited by 86 publications

(68 citation statements)

References 248 publications

(153 reference statements)

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“…Second‐generation DESs incorporate thin stent strut thickness, ideal drug type (ie, “‐limus”), dose, and optimal release kinetics, suggesting that the combination of these components positively influences vascular repair. Although there are differences among sirolimus, everolimus, and biolimus A9 in “position 40,” all of these ‐limus drugs show antiproliferative and immunosuppressive effects that are not able to differentiate endothelial cells from smooth muscle cells; therefore, it is unlikely that the difference in drug affects the extent of reendothelialization 18. The current study reinforces the contention that strut thickness is one of the most important factors in strut coverage; Resolute Integrity also showed better coverage compared with Nobori stents, although it should be noted that stent platform, polymer, and release kinetics are different between Resolute Integrity and Nobori.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Vascular Responses Following New‐Generation Biodegradable and Durable Polymer‐Based Drug‐Eluting Stent Implantation in an Atherosclerotic Rabbit Iliac Artery Model

Nakazawa

Torii

Ijichi

et al. 2016

JAHA

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BackgroundIncomplete endothelialization is the primary substrate of late stent thrombosis; however, recent reports have revealed that abnormal vascular responses are also responsible for the occurrence of late stent failure. The aim of the current study was to assess vascular response following deployment of biodegradable polymer‐based Synergy (Boston Scientific) and Nobori (Terumo) drug‐eluting stents and the durable polymer‐based Resolute Integrity stent (Medtronic) in an atherosclerotic rabbit iliac artery model.Methods and ResultsA total of 24 rabbits were fed an atherogenic diet, and then a balloon injury was used to induce atheroma formation. Synergy, Nobori, and Resolute Integrity stents were randomly implanted in iliac arteries. Animals were euthanized at 28 days for scanning electron microscopic evaluation and at 90 days for histological analysis. The percentage of uncovered strut area at 28 days was lowest with Synergy, followed by Resolute Integrity, and was significantly higher with Nobori stents (Synergy 1.1±2.2%, Resolute Integrity 2.0±3.9%, Nobori 4.6±3.0%; P<0.001). At 90 days, inflammation score was lowest for Synergy (0.27±0.45), followed by Nobori (0.62±0.59), and was highest for Resolute Integrity (0.89±0.46, P<0.001). Foamy macrophage infiltration within neointima (ie, neoatherosclerosis) was significantly less with Synergy (0.62±0.82) compared with Nobori (0.85±0.74) and Resolute Integrity (1.39±1.32; P=0.034).ConclusionsThe biodegradable polymer‐coated thin‐strut Synergy drug‐eluting stent showed the fastest stent strut neointimal coverage and the lowest incidence of neoatherosclerosis in the current animal model.

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Section: Discussionmentioning

confidence: 99%

Comparison of Vascular Responses Following New‐Generation Biodegradable and Durable Polymer‐Based Drug‐Eluting Stent Implantation in an Atherosclerotic Rabbit Iliac Artery Model

Nakazawa

Torii

Ijichi

et al. 2016

JAHA

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“…Calstabins are the cytosolic targets for the immunosuppressant drugs rapamycin and FK506 (Lombardi et al 2017a, 2017b). Rapamycin (or sirolimus) coated on coronary artery stents is used to prevent restenosis by inhibiting vascular smooth muscle migration and proliferation (Santulli 2015; Marks 2003; Santulli and Totary-Jain 2013; Santulli et al 2014). The interaction between calstabins and RyR can regulate the channel and prevent intracellular Ca 2+ leak that leads to a variety of disease states (Santulli and Marks 2015; Huang et al 2006; Brillantes et al 1994a).…”

Section: 7 Modulation Of Ryr Dynamics and Gatingmentioning

confidence: 99%

Ryanodine Receptor Structure and Function in Health and Disease

Santulli

Lewis

Georges

et al. 2018

Subcellular Biochemistry

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117

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Ryanodine receptors (RyRs) are ubiquitous intracellular calcium (Ca2+) release channels required for the function of many organs including heart and skeletal muscle, synaptic transmission in the brain, pancreatic beta cell function, and vascular tone. In disease, defective function of RyRs due either to stress (hyperadrenergic and/or oxidative overload) or genetic mutations can render the channels leaky to Ca2+ and promote defective disease-causing signals as observed in heat failure, muscular dystrophy, diabetes mellitus, and neurodegerative disease. RyRs are massive structures comprising the largest known ion channel-bearing macromolecular complex and exceeding 3 million Daltons in molecular weight. RyRs mediate the rapid release of Ca2+ from the endoplasmic/sarcoplasmic reticulum (ER/SR) to stimulate cellular functions through Ca2+-dependent processes. Recent advances in single-particle cryogenic electron microscopy (cryo-EM) have enabled the determination of atomic-level structures for RyR for the first time. These structures have illuminated the mechanisms by which these critical ion channels function and interact with regulatory ligands. In the present chapter we discuss the structure, functional elements, gating and activation mechanisms of RyRs in normal and disease states.

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“…Mounting evidence has proved that microRNAs control the senescence and dysfunction of endothelial cells, proliferation and migration of vascular smooth muscle cells, and macrophage-drive cytokine production [33]. Among these endothelial microRNAs, the functional roles of miR-126 and miR-143/145 have been well documented [27]. Endothelial cells injury and repair are the fundamental elements in the pathophysiology of atherogenesis.…”

Section: Discussionmentioning

confidence: 99%

Expression Changes of Long Noncoding RNA in the Process of Endothelial Cell Activation

Zhou

Ding

Chen

et al. 2017

Cell Physiol Biochem

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Background: Endothelial cells have been shown to be in response to a variety of local and systemic stimuli, and are able to transition between quiescent and activated states. Endothelial cell activation is critical for the pathogenesis of various cardiovascular diseases. However, the expression changes of long non-coding RNAs (lncRNAs) are still unknown in the process of endothelial cell activation. Thus, this study was aimed to investigate expression changes of lncRNA before and after endothelial cell activation. Materials and Methods: In an experimental model of peripheral venous congestion, endothelial cells were activated and analyzed with Affymetrix HG-U133 plus2.0 microarray. We analyzed these microarray data and reannotated the microarray probes for lncRNA. Results: According to the definition of absolute fold change>2 and p value <0.05, 27 differentially expressed lncRNAs were identified and only 1 lncRNA transcript, ENST00000509256 was down-regualted. Co-expression network of lncRNA and mRNA were constructed to predict function of the dysregulated lncRNA. Gene set enrichment analyses suggested that these ENST00000509256 was associated with many important functions, such as cell-cell signaling and regulation of cell differentiation. Conclusion: Many lncRNAs are dysregulated upon endothelial cell activation and further experiments are needed to identify the potential biological functions of these lncRNAs.

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microRNAs Distinctively Regulate Vascular Smooth Muscle and Endothelial Cells: Functional Implications in Angiogenesis, Atherosclerosis, and In-Stent Restenosis

Cited by 86 publications

References 248 publications

Comparison of Vascular Responses Following New‐Generation Biodegradable and Durable Polymer‐Based Drug‐Eluting Stent Implantation in an Atherosclerotic Rabbit Iliac Artery Model

Comparison of Vascular Responses Following New‐Generation Biodegradable and Durable Polymer‐Based Drug‐Eluting Stent Implantation in an Atherosclerotic Rabbit Iliac Artery Model

Ryanodine Receptor Structure and Function in Health and Disease

Expression Changes of Long Noncoding RNA in the Process of Endothelial Cell Activation

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