MicroRNAs expression profile in solid and unicystic ameloblastomas

Setien-Olarra, Amaia; Marichalar-Mendía, Xabier; Bediaga, Naiara G.; Aguirre-Echebarria, Pablo; Aguirre‐Urízar, José Manuel; Mosqueda‐Taylor, Adalberto

doi:10.1371/journal.pone.0186841

Cited by 13 publications

(11 citation statements)

References 45 publications

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“…ROS are generated in great amount during oxidative stress (i.e. an imbalance between oxidant anti-oxidant agents), a condition in which proteins, carbohydrates, lipids and nucleic acids are affected [82]. The production of ROS is an important defence mechanism against pathogen invasion, with close involvement of bone resorption in the case of endodontic lesions [82][83][84][85][86][87].…”

Section: Other Inflammatory Markersmentioning

confidence: 99%

“…During the process of endodontic infection, the binding of bacterial motifs to Toll-like receptors (TLRs) on the surface of phagocytes leads to the beginning of phagocytosis, inducement of humoral and cellular responses (mediated by lymphocytes B and T, respectively), ROS synthesis and consequent production of inflammatory mediators with cytocines and metalloproteinases (MMPs) [82,83]. The increased expression of ROS-related markers has been associated with the pathogenesis of periapical periodontitis [82][83][84][85][86] at both local and systemic levels [83,[85][86][87].…”

Section: Other Inflammatory Markersmentioning

confidence: 99%

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Inflammatory profile of chronic apical periodontitis: a literature review

Braz‐Silva

Bergamini

Mardegan

et al. 2018

Acta Odontologica Scandinavica

160

129

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Apical periodontitis caused by root canal infection is the most frequent pathological lesion in the jaws, mainly manifested as periapical granulomas and cysts. Understanding of the formation and progression of apical periodontitis as well as the identification of inflammatory biomarkers can help increase the knowledge of pathogenic mechanisms, improve the diagnosis and provide support for different therapeutic strategies. The objective of the present article is to review inflammatory biomarkers such as cytokines, chemokines, inflammatory cells, neuropeptides, RANK/RANKL/OPG system and other inflammatory markers and to relate these systems to the development and progression of pathological conditions related to apical periodontitis.

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Section: Other Inflammatory Markersmentioning

confidence: 99%

Section: Other Inflammatory Markersmentioning

confidence: 99%

Inflammatory profile of chronic apical periodontitis: a literature review

Braz‐Silva

Bergamini

Mardegan

et al. 2018

Acta Odontologica Scandinavica

160

129

View full text Add to dashboard Cite

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“…However, there is a lack of research analyzing the functions of altered miRNAs in AB tissues. Only 1 study analyzed miRNA expression profiles in solid and unicystic AB [20].…”

Section: Introductionmentioning

confidence: 99%

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

et al. 2020

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Background:Ameloblastoma (AB) is a common odontogenic epithelial tumor, with locally invasive behavior and high recurrence. In this study, we hypothesized that miR-524-5p could be involved in the tumor microenvironment by targeting interleukin-33 (IL-33)/suppression of tumorigenicity 2 (ST2) in AB. Material/Methods:The microRNA (miRNA) expression profile of AB tissues and normal oral mucosa tissues (NOM; 6 paired samples) was analyzed. The miRNAs with fold change ³2 and P<0.05 were considered to be differentially expressed. Among them, downregulated miR-524-5p was verified by real-time qPCR. Potential targets of miR-524-5p were predicted by bioinformatics analysis. The expression levels of target genes were detected using real-time qPCR and Western blot, respectively. Immunohistochemistry analysis of target genes was performed, and we also assessed the correlation between miR-524-5p and its target. Results:Microarray analysis results first indicated miR-524-5p is a downregulated miRNA in AB tissues. Real-time qPCR results confirmed the expression pattern of miR-524-5p in AB tissues. Moreover, IL-33 and its receptor ST2 were significantly overexpressed. As shown in immunohistochemistry results, IL-33 was positively expressed in lymphocytes and plasma cells, suggesting that IL-33/ST2 participates in tumor immune responses in the tumor microenvironment. Correlation analysis suggested that miR-524-5p expression was negatively correlated with IL-33/ST2. Conclusions:Our findings reveal that downregulated miR-524-5p can participate in the tumor microenvironment of AB by targeting the IL-33/ST2 axis.

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“…It was shown that the expression of BCL-2 is increased in KOT, but the expression of mir-16–1 and mir-15a are reduced [ 21 ]. Profile of micro-RNA expression emerged 40 micro-RNAs with different expression in ameloblastoma compare to control group [ 22 ].…”

Section: Diagnostic Markers In Odontogenic Tumorsmentioning

confidence: 99%

New diagnostic molecular markers and biomarkers in odontogenic tumors

et al. 2021

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Odontogenic tumors comprised of complex heterogeneous lesions that diverse from harmatomas to malignant tumors with different behavior and histology. The etiology of odontogenic tumors is not exactly determined and pathologists deal with challenges in diagnosis of odontogenic tumors because they are rare and obtained experiences are difficult to evaluate. In this study, we describe immunohistochemical and molecular markers in diagnosis of odontogenic tumors besides advanced diagnostic technique. Immunohistochemical features of odontogenic tumors beside the clinical features and radiological finding can help us to determine the correct diagnosis. Although these markers are neither specific nor sensitive enough, but analysis of gene expression provides definitive confirmation of diagnosis. In addition, “-omics” technology detected specific molecular alternation associated with etiology such as genomics, epigenomics, transcriptomics, proteomics and metabolomics. The post transcriptional events such as DNA methylation and chromatin remodeling by histone modification affect the changes in epigenome. Furthermore, non-coding RNAs like micro-RNAs, long noncoding RNA (lncRNA) and small non-coding RNA (snoRNA) play regulatory role and impact odontogenesis. Molecular marker propose their potential role in etiopathogenesis of odontogenic tumors and suitable candidate in diagnostic, prognostic and therapeutic approaches in addition to patient management. For future evaluations, organoid represents in vitro tumor model-study for tumor behavior, metastasis and invasion, drug screening, immunotherapy, clinical trial, hallmarks association with prognosis and evolution of personalized anti-cancer therapy. Moreover, organoid biobank help us to check genetic profile. We think more investigation and studies are needed to gain these knowledges that can shift therapeutic approaches to target therapy.

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MicroRNAs expression profile in solid and unicystic ameloblastomas

Cited by 13 publications

References 45 publications

Inflammatory profile of chronic apical periodontitis: a literature review

Inflammatory profile of chronic apical periodontitis: a literature review

Downregulated miR-524-5p Participates in the Tumor Microenvironment of Ameloblastoma by Targeting the Interleukin-33 (IL-33)/Suppression of Tumorigenicity 2 (ST2) Axis

New diagnostic molecular markers and biomarkers in odontogenic tumors

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