MicroRNAs in model and complex organisms

Budak, Hikmet; Zhang, Baohong

doi:10.1007/s10142-017-0544-1

Cited by 31 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to their central role in regulating developmental gene expression, plant microRNAs (miRNAs), and more specifically, miRNA-directed gene expression regulation, have more recently been identified as key regulators of plant metabolism, pathogen defense and for a plant to mount an effective adaptive response to abiotic stress [19][20][21]. Alterations to; (1) miRNA accumulation, and/or; (2) miRNA-directed target gene expression regulation have been extensively described in a wide range of plant species following exposure of the plant under study to abiotic stimuli such as drought stress, salt stress, extreme temperature (both elevated and reduced temperatures) and nutrient deficiency [19][20][21][22][23][24][25][26]. Such research has aimed to construct a more detailed molecular understanding of the fundamental, abiotic stress induced, miRNA-directed gene expression networks in plants.…”

Section: Introductionmentioning

confidence: 99%

Profiling of the Differential Abundance of Drought and Salt Stress-Responsive MicroRNAs Across Grass Crop and Genetic Model Plant Species

Pegler¹,

Grof²,

Eamens³

2018

Agronomy

View full text Add to dashboard Cite

In recent years, it has become readily accepted among interdisciplinary agriculturalists that the current global crop yield to land capability ratio is significantly insufficient to achieve food security for the predicted population of 9.5 billion individuals by the year 2050. This issue is further compounded by the: (1) food versus biofuel debate; (2) decreasing availability of arable land; (3) required reductions to the extensive and ongoing environmental damage caused by either poor agricultural practices or agriculture expansion, and; (4) increasingly unfavorable (duration and severity) crop cultivation conditions that accompany man-made climate change, driven by ever-expanding urbanization and its associated industrial practices. Mounting studies are repeatedly highlighting the critical importance of linking genotypes to agronomically beneficial phenotypes and/or using a molecular approach to help address this global crisis, as "simply" clearing the remaining natural ecosystems of the globe for the cultivation of additional, non-modified crops is not efficient, nor is this practice sustainable. The majority of global food crop production is sourced from a small number of members of the Poaceae family of grasses, namely; maize (Zea mays L.), wheat (Triticum aestivum L.) and rice (Oryza sativa L.). It is, therefore, of significant concern that all three of these Poaceae grass species are susceptible to a range of abiotic stresses, including drought and salt stress. Highly conserved among monocotyledonous and dicotyledonous plant species, microRNAs (miRNAs) are now well-established master regulators of gene expression, influencing all aspects of plant development, mediating defense responses against pathogens and adaptation to environmental stress. Here we investigate the variation in the abundance profiles of six known abiotic stress-responsive miRNAs, following exposure to salt and drought stress across these three key Poaceae grass crop species as well as to compare these profiles to those obtained from the well-established genetic model plant species, Arabidopsis thaliana (L.) Heynh. Additionally, we outline the variables that are the most likely primary contributors to instances of differential miRNA abundance across the assessed species following drought or salt stress exposure, specifically; (1) identifying variations in the experimental conditions and/or methodology used to assess miRNA abundance, and; (2) the distribution of regulatory transcription factor binding sites within the putative promoter region of a MICRORNA (MIR) gene that encodes the highly conserved, stress-responsive miRNA. We also discuss the emerging role that non-conserved, species-specific miRNAs play in mediating a plant's response to drought or salt stress.

show abstract

Section: Introductionmentioning

confidence: 99%

Profiling of the Differential Abundance of Drought and Salt Stress-Responsive MicroRNAs Across Grass Crop and Genetic Model Plant Species

Pegler¹,

Grof²,

Eamens³

2018

Agronomy

View full text Add to dashboard Cite

show abstract

“…Additionally it would be of great interest in the future to actively induce changes on a cellular level by induction of mRNA or miRNA (Budak and Zhang 2017) to the perfusion circuit to promote protective protein expressions. Thus, improvement and refinement of COR by future investigations hopefully optimizes preservation of liver allografts before transplantation, which might increase the organs' capability of resistance against ischemia reperfusion injury and will lead to expanded preservation times.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profiles of human livers undergoing rewarming machine perfusion before transplantation—first insights

Hoyer

Treckmann

Benkő

et al. 2021

Funct Integr Genomics

View full text Add to dashboard Cite

Machine perfusion by controlled oxygenated rewarming (COR) is feasible and safe in clinical application and result in a promising outcome. This study utilizes next-generation sequencing (NGS) to investigate the transcriptome of human liver tissue undergoing COR before liver transplantation. Cold-stored livers were subjected to machine-assisted slow COR for ~120 min before transplantation. Biopsies were taken before (preCOR) and after COR (postCOR) and 1 h after reperfusion (postRep). The samples were sequenced, using RNA-seq to analyze differential transcriptional changes between the different stages and treatments of the grafts. Comparison of differential gene expression preCOR and postCOR demonstrated 10 upregulated genes. postRep 97 and 178 genes were upregulated and 7 and 13 downregulated compared to preCOR and postCOR, respectively. A shift of gene expressions by machine perfusion to the TGF-beta pathway was observed. The present study demonstrates distinct transcriptome profiles associated with machine perfusion by COR and transplantation of human livers. Such data provide a deeper understanding of the molecular mechanisms of machine perfusion technology in human liver transplantation.

show abstract

“…The biological functions of sncRNAs, and miRNAs in particular, have been widely explored in recent years. Particularly promising advances in this field were achieved using artificial miRNAs in functional genomics studies [132] and the application of CRISP/Cas9 technology-based approaches for the cloning of miRNA genes [133].…”

Section: Discussionmentioning

confidence: 99%

Small RNA Mobility: Spread of RNA Silencing Effectors and its Effect on Developmental Processes and Stress Adaptation in Plants

Pagliarani

Gambino

2019

IJMS

View full text Add to dashboard Cite

Plants are exposed every day to multiple environmental cues, and tight transcriptome reprogramming is necessary to control the balance between responses to stress and processes of plant growth. In this context, the silencing phenomena mediated by small RNAs can drive transcriptional and epigenetic regulatory modifications, in turn shaping plant development and adaptation to the surrounding environment. Mounting experimental evidence has recently pointed to small noncoding RNAs as fundamental players in molecular signalling cascades activated upon exposure to abiotic and biotic stresses. Although, in the last decade, studies on stress responsive small RNAs increased significantly in many plant species, the physiological responses triggered by these molecules in the presence of environmental stresses need to be further explored. It is noteworthy that small RNAs can move either cell-to-cell or systemically, thus acting as mobile silencing effectors within the plant. This aspect has great importance when physiological changes, as well as epigenetic regulatory marks, are inspected in light of plant environmental adaptation. In this review, we provide an overview of the categories of mobile small RNAs in plants, particularly focusing on the biological implications of non-cell autonomous RNA silencing in the stress adaptive response and epigenetic modifications.

show abstract

MicroRNAs in model and complex organisms

Cited by 31 publications

References 38 publications

Profiling of the Differential Abundance of Drought and Salt Stress-Responsive MicroRNAs Across Grass Crop and Genetic Model Plant Species

Profiling of the Differential Abundance of Drought and Salt Stress-Responsive MicroRNAs Across Grass Crop and Genetic Model Plant Species

Transcriptomic profiles of human livers undergoing rewarming machine perfusion before transplantation—first insights

Small RNA Mobility: Spread of RNA Silencing Effectors and its Effect on Developmental Processes and Stress Adaptation in Plants

Contact Info

Product

Resources

About