microRNAs in Plant Embryogenesis

Alves, Ana; Rodrigues, Andreia S.; Miguel, Celia María Gonzalo

doi:10.1007/978-3-030-35772-6_6

Cited by 6 publications

(3 citation statements)

References 87 publications

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“…The most abundant subclasses of siRNAs, with 24-nt or 21–22-nt, can either lead to transcriptional or post-transcriptional gene silencing depending on if they derive from transposons or repeats (heterochromatic siRNAs), or if they are triggered by specific miRNAs (trans-acting siRNAs) [ 6 ]. The involvement of these classes of molecules in several plant developmental processes, including embryogenesis, and in response to environmental conditions has been often reported and reviewed [ 4 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants

Alves

Cordeiro

Correia

et al. 2021

Plants

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Small non-coding RNAs (sncRNAs) are molecules with important regulatory functions during development and environmental responses across all groups of terrestrial plants. In seed plants, the development of a mature embryo from the zygote follows a synchronized cell division sequence, and growth and differentiation events regulated by highly regulated gene expression. However, given the distinct features of the initial stages of embryogenesis in gymnosperms and angiosperms, it is relevant to investigate to what extent such differences emerge from differential regulation mediated by sncRNAs. Within these, the microRNAs (miRNAs) are the best characterized class, and while many miRNAs are conserved and significantly represented across angiosperms and other seed plants during embryogenesis, some miRNA families are specific to some plant lineages. Being a model to study zygotic embryogenesis and a relevant biotechnological tool, we systematized the current knowledge on the presence and characterization of miRNAs in somatic embryogenesis (SE) of seed plants, pinpointing the miRNAs that have been reported to be associated with SE in angiosperm and gymnosperm species. We start by conducting an overview of sncRNA expression profiles in the embryonic tissues of seed plants. We then highlight the miRNAs described as being involved in the different stages of the SE process, from its induction to the full maturation of the somatic embryos, adding references to zygotic embryogenesis when relevant, as a contribution towards a better understanding of miRNA-mediated regulation of SE.

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Section: Introductionmentioning

confidence: 99%

Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants

Alves

Cordeiro

Correia

et al. 2021

Plants

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“…There are excellent resources on the prominence of microRNAs, and their functions on plant development (Bartel, 2009 ; Rubio-Somoza and Weigel, 2011 ; Wójcikowska et al, 2020 ; Gyawali et al, 2021 ; Ma et al, 2021 ). Several studies highlight the crucial role of miRNA in plant development, especially focusing on embryogenesis, including SE (Armenta-Medina et al, 2017 ; Liu et al, 2018 ; Song et al, 2019 ; Alves et al, 2020 ; Wójcikowska et al, 2020 ). Here, we emphasize how AGL15, in addition to targeting protein-encoding genes, also regulates MIR genes.…”

Section: Agl15 Directly Binds Genes Encoding Micro-rnasmentioning

confidence: 99%

AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism

et al. 2022

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Plants have amazing regenerative properties with single somatic cells, or groups of cells able to give rise to fully formed plants. One means of regeneration is somatic embryogenesis, by which an embryonic structure is formed that “converts” into a plantlet. Somatic embryogenesis has been used as a model for zygotic processes that are buried within layers of maternal tissues. Understanding mechanisms of somatic embryo induction and development are important as a more accessible model for seed development. We rely on seed development not only for most of our caloric intake, but also as a delivery system for engineered crops to meet agricultural challenges. Regeneration of transformed cells is needed for this applied work as well as basic research to understand gene function. Here we focus on a MADS-domain transcription factor, AGAMOUS-Like15 (AGL15) that shows a positive correlation between accumulation levels and capacity for somatic embryogenesis. We relate AGL15 function to other transcription factors, hormones, and epigenetic modifiers involved in somatic embryo development.

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“…A major issue in plant developmental analysis is to unravel the mechanisms that operate during embryogenesis that enable a plant to specify its body plan through tissue differentiation patterns. Research in the last decade has demonstrated that miRNAs have crucial roles during plant embryogenesis [ 40 ]. Plant miRNAs tend to target the genes encoding the key developmental regulators among which are many transcription factors (TFs) [ 41 ], which have been described as extremely important for ZE and SE [ 41 , 42 ].…”

Section: Plant Mirnas What We Are Dealing With?mentioning

confidence: 99%

Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis

Wójcik

2020

IJMS

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During early plant embryogenesis, some of the most fundamental decisions on fate and identity are taken making it a fascinating process to study. It is no surprise that higher plant embryogenesis was intensively analysed during the last century, while somatic embryogenesis is probably the most studied regeneration model. Encoded by the MIRNA, short, single-stranded, non-coding miRNAs, are commonly present in all Eukaryotic genomes and are involved in the regulation of the gene expression during the essential developmental processes such as plant morphogenesis, hormone signaling, and developmental phase transition. During the last few years dedicated to miRNAs, analytical methods and tools have been developed, which have afforded new opportunities in functional analyses of plant miRNAs, including (i) databases for in silico analysis; (ii) miRNAs detection and expression approaches; (iii) reporter and sensor lines for a spatio-temporal analysis of the miRNA-target interactions; (iv) in situ hybridisation protocols; (v) artificial miRNAs; (vi) MIM and STTM lines to inhibit miRNA activity, and (vii) the target genes resistant to miRNA. Here, we attempted to summarise the toolbox for functional analysis of miRNAs during plant embryogenesis. In addition to characterising the described tools/methods, examples of the applications have been presented.

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microRNAs in Plant Embryogenesis

Cited by 6 publications

References 87 publications

Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants

Small Non-Coding RNAs at the Crossroads of Regulatory Pathways Controlling Somatic Embryogenesis in Seed Plants

AGL15 Promotion of Somatic Embryogenesis: Role and Molecular Mechanism

Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis

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