MicroRNAs in regulation of pluripotency and somatic cell reprogramming

Tian, Weihua; Shi, San-bao; Sha, Hao

doi:10.4161/rna.25828

Cited by 22 publications

(15 citation statements)

References 79 publications

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“…Strikingly, the differences in the expression of these genes among the samples analyzed did not correlate with the differences in the miRNA populations. This indicates that maybe the differences observed in miRNA population among PGCs are due to other biological processes such as turnover or stockpile accumulations as has been already described (Wang et al 2013); and/or by the presence of other RNAs that may act as miRNA sponges or "competing endogenous RNAs" (ceRNAs) (Kartha and Subramanian 2014). Also, the expression of miRNA biogenesis genes, such as Drosha, Dgcr8, Xpo5, and Ago2 (except for Ago2 in E13.5 male PGCs) decays accordingly with the differentiation state of the cells.…”

Section: Characterization Of Mirna Biogenesis In the Developing Gonadsupporting

confidence: 57%

“…miR-17-92 is known to be the key regulator in spermatogenesis (Tong et al 2012;Xie et al 2016), being down-regulated in the presence of RA (Beveridge et al 2009). In addition, it participates in the regulation of pluripotency (for review, see Wang et al 2013). These data indicate that these miRNAs are key for the development and sexual differentiation of PGCs.…”

Section: Males Vs Femalesmentioning

confidence: 99%

“…Since this miRNA is up-regulated in female PGCs, depletion of PRC2 may enhance the response to RA in females, while in males it would be inhibited. The other member of this cluster, miR-199a, has been described as an inhibitor of pluripotency by enhancing the expression of p53 (Wang et al 2013). Interestingly, Nanog, a pluripotency associated gene, is naturally down-regulated at early stages of development in female PGCs (Yamaguchi et al 2005), which could explain these differences in the regulation of pluripotency between males and females during this developmental window.…”

Section: Mirnas Participating In Meiotic Entry Regulationmentioning

confidence: 99%

“…In contrast, the let-7 family is up-regulated in somatic cells with respect to PGCs. The let-7 family induces a more differentiated (less pluripotent) state by targeting transcripts such as N-myc and C-myc, while cluster miR-290-295 supports a pluripotent state by targeting p27 and Last2 transcripts (Wang et al 2013).…”

Section: Mirnas Participating In Meiotic Entry Regulationmentioning

confidence: 99%

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MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development

Fernández-Pérez

Brieño‐Enríquez

Isoler-Alcaraz

et al. 2017

RNA

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In mammals, commitment and specification of germ cell lines involves complex programs that include sex differentiation, control of proliferation, and meiotic initiation. Regulation of these processes is genetically controlled by fine-tuned mechanisms of gene regulation in which microRNAs (miRNAs) are involved. We have characterized, by small-RNA-seq and bioinformatics analyses, the miRNA expression patterns of male and female mouse primordial germ cells (PGCs) and gonadal somatic cells at embryonic stages E11.5, E12.5, and E13.5. Differential expression analyses revealed differences in the regulation of key miRNA clusters such as ,, and , whose targets have defined roles during gonadal sexual determination in both germ and somatic cells. Extensive analyses of miRNA sequences revealed an increase in noncanonical isoforms on PGCs at E12.5 and dramatic changes of 3' isomiR expression and 3' nontemplate nucleotide additions in female PGCs at E13.5. Additionally, RT-qPCR analyses of genes encoding proteins involved in miRNA biogenesis and 3' nucleotide addition uncovered sexually and developmentally specific expression, characterized by the decay of, , and expression along gonadal development. These results demonstrate that miRNAs, their isomiRs, and miRNA machinery are differentially regulated and participate actively in gonadal sexual differentiation in both PGCs and gonadal somatic cells.

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Section: Characterization Of Mirna Biogenesis In the Developing Gonadsupporting

confidence: 57%

Section: Males Vs Femalesmentioning

confidence: 99%

Section: Mirnas Participating In Meiotic Entry Regulationmentioning

confidence: 99%

Section: Mirnas Participating In Meiotic Entry Regulationmentioning

confidence: 99%

See 2 more Smart Citations

MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development

Fernández-Pérez

Brieño‐Enríquez

Isoler-Alcaraz

et al. 2017

RNA

View full text Add to dashboard Cite

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“…Morphologically, the miR‐371 has been detected in the tissue of testicular GCTs (Palmer et al , ; Dieckmann et al , ; Vilela‐Salgueiro et al , ) and little amounts of the miRNA are obviously also produced in healthy testicular tissue, since the miR‐371a‐3p expression was 4.8‐fold higher in the testicular vein blood than in the cubital vein blood of non‐tumor patients (Dieckmann et al , ). As the miR‐371‐3 cluster is typically expressed in embryonic stem cells (Stadler et al , ; Pfaff et al , ; Langroudi et al , ) and as it represents one of the known key regulators of the metabolism of pluripotent stem cells (Suh et al , ; Wang et al , ; Lee et al , ) it was rational to assume that these miRNAs would be present in body fluids that are in close contact with germ cells or germ cell related cells like spermatozoa. Accordingly, a high expression of miR‐371a‐3p was shown in the seminal plasma of five healthy men (Spiekermann et al , ).…”

Section: Introductionmentioning

confidence: 99%

Expression of miRNA‐371a‐3p in seminal plasma and ejaculate is associated with sperm concentration

et al. 2019

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Background: The microRNAs of the miR-371-3 cluster are novel serum markers for testicular germ cell tumors. Sporadic reports suggested the expression of this miRNA in semen. Objectives: To verify the expression of miR-371a-3p in seminal plasma and unprocessed ejaculate; to compare seminal plasma miRNA levels in germ cell tumors patients with those of controls; to look for an association of miRNA levels with semen quality. Materials and methods: The miR-371a-3p expression was analyzed with qPCR. The study population consisted of 100 participants: seminal plasma samples from 20 germ cell tumors patients and 30 controls, serum samples from 12 healthy men, ejaculate samples from 38 men undergoing fertility testing. Results: The seminal plasma miR-371a-3p levels of germ cell tumors patients were not different from controls. The miRNA expression was very low in serum but much higher in seminal plasma. In ejaculate samples, the miRNA expression significantly correlated with sperm concentration and the total sperm count. Discussion: miR-371-a-3p is present in sperm-containing fluids. Seminal plasma levels cannot be used to distinguish germ cell tumors from controls. The correlation with sperm concentration in ejaculate samples suggests the spermatozoa as possible source of miR-371a-3p production. Conclusion: The miR-371a-3p levels in ejaculate could represent a novel biomarker for the non-invasive evaluation of male infertility.

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Cardiac Regeneration and microRNAs: Regulators of Pluripotency, Reprogramming, and Cardiovascular Lineage Commitment

Doeleman

Feyen

Mestdagh

et al. 2015

Stem Cell Biology and Regenerative Medicine

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MicroRNAs in regulation of pluripotency and somatic cell reprogramming

Cited by 22 publications

References 79 publications

MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development

MicroRNA dynamics at the onset of primordial germ and somatic cell sex differentiation during mouse embryonic gonad development

Expression of miRNA‐371a‐3p in seminal plasma and ejaculate is associated with sperm concentration

Cardiac Regeneration and microRNAs: Regulators of Pluripotency, Reprogramming, and Cardiovascular Lineage Commitment

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