MicroRNAs: new candidates for the regulation of the human cumulus–oocyte complex

Assou, Saïd; Al-Edani, T.; Haouzi, D.; Naveilhan, Philippe; Lecellier, Charles-Henri; Piquemal, David; Commes, Thérèse; Aı̈t-Ahmed, Ounissa; Déchaud, H.; Hamamah, S.

doi:10.1093/humrep/det321

Cited by 101 publications

(80 citation statements)

References 68 publications

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“…To our knowledge, this is the first human study to characterize global miRNA expression in FF according to the degree of maturation of the oocyte retrieved while also excluding pathologies that could potentially bias the miRNA profile. Different studies have focused on characterizing FF miRNAs from patients with different reproductive pathologies such as premature ovarian failure (POF) or PCOS (20,21,40) in cumulus cells obtained from uncharacterized IVF patients or unfertilized oocytes (41). However, the presence of such pathologies could represent a source of bias since the role of different miRNAs has already been related to diverse genital tract dysfunctions (42).…”

Section: Discussionmentioning

confidence: 99%

Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

Moreno

Núñez

Quiñonero

et al. 2015

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

Moreno

Núñez

Quiñonero

et al. 2015

Fertility and Sterility

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“…Recently, several studies have shown that miRNA may play an important role in female fertility and in the regulation of oocyte and CC crosstalk (Mishima et al, 2008;Assou et al, 2013;Donadeu and Schauer, 2013;Velthut-Meikas et al, 2013). However, to the best of our knowledge, no study has tested the involvement of other noncoding RNAs in folliculogenesis and ovulation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Yerushalmi

Salmon‐Divon

Yung

et al. 2014

MHR: Basic Science of Reproductive Medicine

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Cumulus expansion and oocyte maturation are central processes in ovulation. Knowledge gained from rodent and other mammalian models has revealed some of the molecular pathways associated with these processes. However, the equivalent pathways in humans have not been thoroughly studied and remain unidentified. Compact cumulus cells (CCs) from germinal vesicle cumulus oocyte complexes (COCs) were obtained from patients undergoing in vitro maturation (IVM) procedures. Expanded CCs from metaphase 2 COC were obtained from patients undergoing IVF/ICSI. Global transcriptome profiles of the samples were obtained using state-of-the-art RNA sequencing techniques. We identified 1746 differentially expressed (DE) genes between compact and expanded CCs. Most of these genes were involved in cellular growth and proliferation, cellular movement, cell cycle, cell-to-cell signaling and interaction, extracellular matrix and steroidogenesis. Out of the DE genes, we found 89 long noncoding RNAs, of which 12 are encoded within introns of genes known to be involved in granulosa cell processes. This suggests that unique noncoding RNA transcripts may contribute to the regulation of cumulus expansion and oocyte maturation. Using global transcriptome sequencing, we were able to generate a library of genes regulated during cumulus expansion and oocyte maturation processes. Analysis of these genes allowed us to identify important new genes and noncoding RNAs potentially involved in COC maturation and cumulus expansion. These results may increase our understanding of the process of oocyte maturation and could ultimately improve the efficacy of IVM treatment.

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“…The maturation, development and quality of oocytes are provided through cumulus cells by growth factors, paracrine signals, secondary messenger and nutrient exchanges [3,5,17,22,37,41]. In addition, cumulus cells are involved in extracellular matrix and apoptosis via microRNAs (MIR29a, MIR30d, MIR21, MIR93, MIR320a, MIR125a and the LET7 family) [7].…”

Section: Introductionmentioning

confidence: 99%

Identification of the key regulating genes of diminished ovarian reserve (DOR) by network and gene ontology analysis

2016

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Diminished ovarian reserve (DOR) is one of the reasons for infertility that not only affects both older and young women. Ovarian reserve assessment can be used as a new prognostic tool for infertility treatment decision making. Here, up- and down-regulated gene expression profiles of granulosa cells were analysed to generate a putative interaction map of the involved genes. In addition, gene ontology (GO) analysis was used to get insight intol the biological processes and molecular functions of involved proteins in DOR. Eleven up-regulated genes and nine down-regulated genes were identified and assessed by constructing interaction networks based on their biological processes. PTGS2, CTGF, LHCGR, CITED, SOCS2, STAR and FSTL3 were the key nodes in the up-regulated networks, while the IGF2, AMH, GREM, and FOXC1 proteins were key in the down-regulated networks. MIRN101-1, MIRN153-1 and MIRN194-1 inhibited the expression of SOCS2, while CSH1 and BMP2 positively regulated IGF1 and IGF2. Ossification, ovarian follicle development, vasculogenesis, sequence-specific DNA binding transcription factor activity, and golgi apparatus are the major differential groups between up-regulated and down-regulated genes in DOR. Meta-analysis of publicly available transcriptomic data highlighted the high coexpression of CTGF, connective tissue growth factor, with the other key regulators of DOR. CTGF is involved in organ senescence and focal adhesion pathway according to GO analysis. These findings provide a comprehensive system biology based insight into the aetiology of DOR through network and gene ontology analyses.

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MicroRNAs: new candidates for the regulation of the human cumulus–oocyte complex

Abstract: Not applicable.

Cited by 101 publications

References 68 publications

Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Identification of the key regulating genes of diminished ovarian reserve (DOR) by network and gene ontology analysis

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