MicroRNAs: potential biomarkers for disease diagnosis

Xu, Li; Qi, Xue; Duan, Shengliang; Xie, Yumei; Ren, Xinlei; Chen, Gaoyun; Yang, Xiaobo; Han, Lili; Dong, Qirui

doi:10.3233/bme-141223

Cited by 21 publications

(13 citation statements)

References 62 publications

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“…Free exRNA represents a substantial proportion of RNA in the circulation [ 33 – 38 ]. It is receiving much attention as potential biomarker of various diseases [ 39 , 40 ]. However, there are no prior reports nor has it been mentioned in reviews [ 41 – 43 ] that free exRNA can transfer functional information to acceptor cells.…”

Section: Discussionmentioning

confidence: 99%

Free Extracellular miRNA Functionally Targets Cells by Transfecting Exosomes from Their Companion Cells

et al. 2015

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Lymph node and spleen cells of mice doubly immunized by epicutaneous and intravenous hapten application produce a suppressive component that inhibits the action of the effector T cells that mediate contact sensitivity reactions. We recently re-investigated this phenomenon in an immunological system. CD8+ T lymphocyte-derived exosomes transferred suppressive miR-150 to the effector T cells antigen-specifically due to exosome surface coat of antibody light chains made by B1a lymphocytes. Extracellular RNA (exRNA) is protected from plasma RNases by carriage in exosomes or by chaperones. Exosome transfer of functional RNA to target cells is well described, whereas the mechanism of transfer of exRNA free of exosomes remains unclear. In the current study we describe extracellular miR-150, extracted from exosomes, yet still able to mediate antigen-specific suppression. We have determined that this was due to miR-150 association with antibody-coated exosomes produced by B1a cell companions of the effector T cells, which resulted in antigen-specific suppression of their function. Thus functional cell targeting by free exRNA can proceed by transfecting companion cell exosomes that then transfer RNA cargo to the acceptor cells. This contrasts with the classical view on release of RNA-containing exosomes from the multivesicular bodies for subsequent intercellular targeting. This new alternate pathway for transfer of exRNA between cells has distinct biological and immunological significance, and since most human blood exRNA is not in exosomes may be relevant to evaluation and treatment of diseases.

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Section: Discussionmentioning

confidence: 99%

Free Extracellular miRNA Functionally Targets Cells by Transfecting Exosomes from Their Companion Cells

et al. 2015

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“…MiRNAs are a class of small regulatory RNA molecules, 21-23 nucleotides in length, that negatively regulate the expression of their target genes by binding to the 3′-untranslated regions (UTR) of the corresponding mRNAs [ 3 ]. Previous studies have reported that miRNAs can act as oncogenes or tumor suppressors during the development and metastasis of osteosarcoma [ 4 , 5 ]. MiR-93 reportedly promotes the metastasis of osteosarcoma by targeting E2F1 [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-542-5p is a negative prognostic factor and promotes osteosarcoma tumorigenesis by targeting HUWE1

Cheng

et al. 2015

Oncotarget

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Recent evidence has demonstrated that microRNAs (miRNAs) are involved in the proliferation and metastasis of osteosarcoma. Using miRNA microarray and functional screening methods to compare miRNA expression profiles in osteosarcoma cell lines treated with Trichostatin A (TSA), overexpression of miR-542-5p was determined to be involved in the proliferation of osteosarcoma. We used isobaric tags for relative and absolute quantitation (iTRAQ) and nanoscale liquid chromatography-mass spectrometry (NanoLC−MS/MS) to identify differentially expressed proteins in MNNG/HOS and U2OS osteosarcoma cell lines transfected with miR-542-5p; in both cell lines, seven proteins were downregulated, and nine were upregulated. HUWE1 was found to be a direct target of miR-542-5p in both osteosarcoma cell lines, and was negatively correlated with miR-542-5p levels in human osteosarcoma tissues. Moreover, the expression of miR-542-5p was upregulated in human osteosarcoma tissue compared with non-tumor adjacent tissue. Kaplan-Meier analysis revealed that overexpression of miR-542-5p predicted poor prognosis for osteosarcoma patients. Taken together, our results indicated that miR-542-5p plays a critical role in the proliferation of osteosarcoma and targets HUWE1.

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“…Over the last few years, miRNA studies have increased and pointed out these molecules as excellent biomarkers for different pathologies [21]. Importantly, miRNAs have especially been found altered in different cancers [22,23].…”

Section: Discussionmentioning

confidence: 99%

Validation of miR-1228-3p as Housekeeping for MicroRNA Analysis in Liquid Biopsies from Colorectal Cancer Patients

et al. 2019

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Background: Circulating microRNA (miRNA) analysis is a growing research field. However, it usually requires an endogenous control or housekeeping (HK) in order to normalize expression of specific miRNAs throughout different samples. Unfortunately, no adequate HK for circulating miRNA analysis is still known in the colorectal cancer (CRC) context whereas several have been suggested. Hence, our aims were to validate the previously suggested miR-1228-3p as HK for CRC studies, to compare its suitability with the widely used miR-16-5p, and to evaluate the influence of hemolysis on both miRNAs. Methods: We analyzed by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) the expression of miR-1228-3p, miR-16-5p and the spike-in cel-miR-39 in a set of 297 plasmas (92 CRC, 101 advanced adenomas -AA-, and 100 controls) and 213 serum samples (59 CRC, 74 AA and 80 controls). We also analyzed both miRNAs depending on the hemolysis degree in 7 plasmas and 31 serums. Results: Levels of miR-1228-3p and miR-16-5p did not show significant differences between groups although miR-16-5p exhibited more variability in plasma and serum samples. Importantly, the combination of cel-miR-39 and miR-1228-3p was the most stable one. Moreover, we observed that miR-16-5p was significantly influenced by hemolysis in contrast with miR-1228-3p that exhibited no correlation with this confounding factor in both biofluids. Conclusion: MiR-1228-3p has been validated as an adequate endogenous control for circulating miRNA analysis in CRC and AA liquid biopsies.

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MicroRNAs: potential biomarkers for disease diagnosis

Cited by 21 publications

References 62 publications

Free Extracellular miRNA Functionally Targets Cells by Transfecting Exosomes from Their Companion Cells

Free Extracellular miRNA Functionally Targets Cells by Transfecting Exosomes from Their Companion Cells

MiR-542-5p is a negative prognostic factor and promotes osteosarcoma tumorigenesis by targeting HUWE1

Validation of miR-1228-3p as Housekeeping for MicroRNA Analysis in Liquid Biopsies from Colorectal Cancer Patients

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