microRNAs related to angiogenesis are dysregulated in endometrioid endometrial cancer

Ramón, Luis A.; Braza-Boïls, Aitana; Gilabert, J.; Chirivella, Melitina; España, Francisco; Estellés, Amparo; Gilabert-Estellés, Juan

doi:10.1093/humrep/des292

Cited by 55 publications

(41 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inconsistent results have been reported for the role of miR-17 so far. While some investigatory approaches hypothesize an anti-angiogenetic activity (41,51), other studies account for either pro-angiogenetic activity (83,84) or no association with VEGF-A expression (65). Our data identified a significant hypoxia-driven expression reduction of miR20a in Ishikawa cells.…”

Section: Discussionmentioning

confidence: 46%

“…Anti-angiogenetic activity of miR-20a by targeting VEGF-A has been reported (41,65). Inconsistent results have been reported for the role of miR-17 so far.…”

Section: Discussionmentioning

confidence: 99%

“…The miR-17-92 cluster, which contains six mature miRNAs (miR-17, -18a, -19a, -19b, -20a and -92a), conducts a complex role in angiogenetic signaling cascades (65) In the present analysis, we evaluated four specific miRNAs of this cluster: miR-17, miR-19b, miR-20a and miR-92a. Anti-angiogenetic activity of miR-20a by targeting VEGF-A has been reported (41,65).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Hypoxia- and acidosis-driven aberrations of secreted microRNAs in endometrial cancer in vitro

et al. 2017

View full text Add to dashboard Cite

Abstract. Due to their post-transcriptional regulatory impact on gene expression, microRNAs (miRNA, miRs) influence decisively cellular processes of differentiation, proliferation and apoptosis. In oncogenic pathways various miRNAs exert either oncogenic or tumor suppressor activities in a stage-specific manner. Dysregulation of miRNA expression pattern has been associated with several human cancers including endometrial cancer (EC). In the present study, expression profile alterations of EC associated secreted miRNAs were determined under the microenvironmental stress situations hypoxia and acidosis occurring in tumor progression and metastasis. The potential influence of hypoxia and acidosis vs. control conditions on the expression levels of 24 EC-relevant miRNA types was quantitatively accessed via real-time PCR in three established EC in vitro models. Expression data were analyzed statistically. In vitro application of hypoxia resulted in downregulation of miR-15a, miR-20a, miR-20b and miR-128-1 in Ishikawa cells (type I EC) and upregulation of miR-21 in EFE-184 cells (type I EC). Acidosis triggered upregulation of tumor promoting miR125b in AN3-CA cell (type II EC), whereas in Ishikawa cells (type I EC) miRNAs with tumor suppressive function were found altered in divergent directions, both up-(let-7a) and down-(miR-22) regulated. Our current findings emphasize the functional importance of secreted miRNAs in the immediate response of EC cells to exogenic stress situations such as the typical tumor epiphenomena hypoxia and acidosis. Focusing on the specific potential of secreted, thus circulating miRNA molecules, alterations in expression levels not only influence intracellular gene expression and signaling cascades, but also transfer the induction of (tumor)biological cellular changes to adjacent cells.

show abstract

Section: Discussionmentioning

confidence: 46%

“…Anti-angiogenetic activity of miR-20a by targeting VEGF-A has been reported (41,65). Inconsistent results have been reported for the role of miR-17 so far.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hypoxia- and acidosis-driven aberrations of secreted microRNAs in endometrial cancer in vitro

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The age-standardised incidence is 9.1 per 100,000 women per year in more developed countries, compared with 1.7 per 100,000 per year in less developed countries (3). The dysregulation of oncogenes and/or tumor suppressors has been demonstrated to serve a role in EC metastasis (1,4). Therefore, the development of potential molecular targets indicates promise for the treatment of EC progression.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1

Zhao

Zhu

et al. 2015

Oncology Letters

View full text Add to dashboard Cite

Abstract. MicroRNAs (miRs) have been demonstrated to serve important roles in the development and progression of human cancer, primarily through the direct targeting of oncogenes or tumor suppressors. It has been previously suggested that miR-126 may be associated with endometrial cancer (EC). However, the exact role of miR-126 in the migration and invasion of EC cells has not yet been studied. The present study demonstrated that the expression of miR-126 was significantly decreased in EC tissues when compared with matched normal adjacent tissues. The current study reverse transcriptionquantitative polymerase chain reaction was performed in order to examine the expression level of miR-126. Wound healing and transwell assays were used to examine cell migration and invasion. A luciferase reporter assay was used to determine the targeting relationship and western blotting assay was performed to detect the protein expression. Furthermore, the overexpression of miR-126 significantly inhibited EC SKOV3 cell migration and invasion. Molecular mechanism investigation established that insulin receptor substrate 1 (IRS1) functioned as a direct miR-126 target, and its expression was negatively regulated by miR-126 at a post-transcriptional level in the SKOV3 cells. Additionally, the overexpression of IRS1 reversed the inhibitory effect of miR-126 overexpression on SKOV3 cell migration and invasion. In conclusion, the current study demonstrated that miR-126 inhibited EC cell migration and invasion, at least partially through the direct targeting of IRS1, suggesting that miR-126 may aid the treatment of EC metastasis.

show abstract

“…Otsuka et al (31) studied the luteal functions of infertile mice, and further demonstrated that miR-17-5p could affect the endothelial cell functions via the inhibition of metalloproteinase 1 (TIMP-1), thus promoting angiogenesis. Furthermore, previous studies have indicated that miR-17-5p plays a role in the promotion of angiogenesis (32,33). However, previous studies regarding the angiogenic promotion effects of miR-17-5p have not investigated its expression in wounds.…”

Section: A B C D a B Cmentioning

confidence: 99%

Effect of vacuum sealing drainage on the expression of VEGF and miRNA-17-5p in seawater-immersed blast-injury wounds

Yang¹,

Shi²,

Cao³

2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Abstract. The aim of the present study was to compare and observe the expression levels of vascular endothelial growth factor (VEGF) and miRNA-17-5p during the treatment of seawater-immersed blast-injury wounds (SIBIW) under different conditions of vacuum sealing drainage (VSD), and to identify the optimized range of VSD treatment and partially explain its mechanisms. The bilateral hips and scapulae of experimental pigs (weight, 25-30 kg) were subjected to blast-injury wounds, followed by the seawater immersion. The animals then underwent conventional dressing treatment under 120, 180 and 240 mmHg VSD. Visual observation, in addition to histological, immunohistochemical and molecular biological techniques were applied to compare and observe the extent of wound healing and expression levels of VEGF and miRNA-17-5p. The wound healing of the VSD treatment group was improved compared with the control group, with 120 mmHg negative pressure producing the most marked effect. miR-17-5p expression was detected in the SIBIW granulation tissues. There was significant difference between each VSD treatment group and control group at each time point (P<0.05). Thus, the present results show that miR-17-5p can be expressed in SIBIW granulation tissues, and this effect is most evident under 120 mmHg negative pressure, which may inform the optimized negative range for the treatment of SIBIW.

show abstract

microRNAs related to angiogenesis are dysregulated in endometrioid endometrial cancer

Cited by 55 publications

References 64 publications

Hypoxia- and acidosis-driven aberrations of secreted microRNAs in endometrial cancer in vitro

Hypoxia- and acidosis-driven aberrations of secreted microRNAs in endometrial cancer in vitro

MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1

Effect of vacuum sealing drainage on the expression of VEGF and miRNA-17-5p in seawater-immersed blast-injury wounds

Contact Info

Product

Resources

About