MicroRNAs secreted by human embryos could be potential biomarkers for clinical outcomes of assisted reproductive technology

Fang, Fang; Li, Zili; Yu, Jiangyu; Long, Yuting; Zhao, Qian; Ding, Xiaofang; Wu, Lin; Shao, Shumin; Zhang, Ling; Xiang, Wenpei

doi:10.1016/j.jare.2021.01.003

Cited by 37 publications

(24 citation statements)

References 62 publications

(71 reference statements)

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“…The model with the minimum AIC value was the preferred model, and genera in this model were viewed as the potential biomarkers. Receiver operating characteristic (ROC) curve analysis was conducted to evaluate the diagnostic performance of the identified potential biomarkers ( Kumstel et al., 2020 ; Wang et al., 2021 ; Fang et al., 2021 ). The area under the curve (AUC) was used to assess the diagnostic performance: 0.9–1, excellent; 0.8–0.9, good; 0.7–0.8, fair; 0.6–0.7, poor; and 0.5–0.6, failed.…”

Section: Methodsmentioning

confidence: 99%

Differential Gut Microbiota Compositions Related With the Severity of Major Depressive Disorder

Zhong

Chen

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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ObjectiveIncreasing evidence shows a close relationship between gut microbiota and major depressive disorder (MDD), but the specific mechanisms remain unknown. This study was conducted to explore differential gut microbiota compositions related to the severity of MDD.MethodsHealthy controls (HC) (n = 131) and MDD patients (n = 130) were included. MDD patients with Hamilton Depression Rating Scale (HDRS) score <25 and ≥25 were assigned into moderate (n = 72) and severe (n = 58) MDD groups, respectively. Univariate and multivariate analyses were used to analyze the gut microbiota compositions at the genus level.ResultsThirty-six and 27 differential genera were identified in moderate and severe MDD patients, respectively. The differential genera in moderate and severe MDD patients mainly belonged to three (Firmicutes, Actinobacteriota, and Bacteroidota) and two phyla (Firmicutes and Bacteroidota), respectively. One specific covarying network from phylum Actinobacteriota was identified in moderate MDD patients. In addition, five genera (Collinsella, Eggerthella, Alistipes, Faecalibacterium, and Flavonifractor) from the shared differential genera by two MDD groups had a fair efficacy in diagnosing MDD from HC (AUC = 0.786).ConclusionsOur results were helpful for further exploring the role of gut microbiota in the pathogenesis of depression and developing objective diagnostic methods for MDD.

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Section: Methodsmentioning

confidence: 99%

Differential Gut Microbiota Compositions Related With the Severity of Major Depressive Disorder

Zhong

Chen

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Then, in 2019, Abu-Halima and his colleagues reported that the abundance of miR-19b-3p was significantly lower in spent culture medium (SCM) samples associated with positive pregnancy (12). Fang et al indicated that hsa-miR-26b-5p and hsa-miR-21-5p could serve as potential biomarkers for reproductive outcomes by detecting the miRNA profiles in the human embryo culture media (13).…”

Section: Introductionmentioning

confidence: 99%

Characterization of microRNAs in spent culture medium associated with human embryo quality and development

Wang¹,

Chen²,

Zhu³

et al. 2021

Ann Transl Med

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“…In their study, pregnancy outcomes and miRNA profiles were correlated only when intracytoplasmic sperm injection (ICSI) was used, which could limit the use of miRNA as a biological marker for pregnancy outcomes [ 22 ]. Fang et al also concluded that certain miRNA molecules (namely, has-miR-26b-5p and has-miR-21b-5p) could serve as potential biomarkers for reproductive outcomes [ 23 ].…”

Section: Emerging Biological Markers In Secmmentioning

confidence: 99%

“…These authors cumulatively agree that miRNA could serve as a good non-invasive marker of the quality of the preimplantation embryo, as it is stable, easily accessible, and easily detected. More research is needed to standardize the tests and to better understand the role of these molecules [ 22 , 23 , 24 ].…”

Section: Emerging Biological Markers In Secmmentioning

confidence: 99%

Non-Invasive Preimplantation Genetic Testing for Aneuploidy and the Mystery of Genetic Material: A Review Article

Tomic

Bokal

Štimpfel

2022

IJMS

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This review focuses on recent findings in the preimplantation genetic testing (PGT) of embryos. Different preimplantation genetic tests are presented along with different genetic materials and their analysis. Original material concerning preimplantation genetic testing for aneuploidy (PGT-A) was sourced by searching the PubMed and ScienceDirect databases in October and November 2021. The searches comprised keywords such as ‘preimplantation’, ‘cfDNA’; ‘miRNA’, ‘PGT-A’, ‘niPGT-A’, ‘aneuploidy’, ‘mosaicism’, ‘blastocyst biopsy’, ‘blastocentesis’, ‘blastocoel fluid’, ‘NGS’, ‘FISH’, and ‘aCGH’. Non-invasive PGT-A (niPGT-A) is a novel approach to the genetic analysis of embryos. The premise is that the genetic material in the spent embryo culture media (SECM) corresponds to the genetic material in the embryo cells. The limitations of niPGT-A are a lower quantity and lesser quality of the cell-free genetic material, and its unknown origin. The concordance rate varies when compared to invasive PGT-A. Some authors have also hypothesized that mosaicism and aneuploid cells are preferentially excluded from the embryo during early development. Cell-free genetic material is readily available in the spent embryo culture media, which provides an easier, more economic, and safer extraction of genetic material for analysis. The sampling of the SECM and DNA extraction and amplification must be optimized. The origin of the cell-free media, the percentage of apoptotic events, and the levels of DNA contamination are currently unknown; these topics need to be further investigated.

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MicroRNAs secreted by human embryos could be potential biomarkers for clinical outcomes of assisted reproductive technology

Abstract: Graphical abstract

Cited by 37 publications

References 62 publications

Differential Gut Microbiota Compositions Related With the Severity of Major Depressive Disorder

Differential Gut Microbiota Compositions Related With the Severity of Major Depressive Disorder

Characterization of microRNAs in spent culture medium associated with human embryo quality and development

Non-Invasive Preimplantation Genetic Testing for Aneuploidy and the Mystery of Genetic Material: A Review Article

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