MicroRNAs: tiny molecules with a significant role in mammalian follicular and oocyte development

Tesfaye, Dawit; Gebremedhn, Samuel; Salilew‐Wondim, Dessie; Hailay, Tsige; Hoelker, M.; Große‐Brinkhaus, Christine; Schellander, K.

doi:10.1530/rep-17-0428

Cited by 81 publications

(71 citation statements)

References 174 publications

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“…Consequently, when FSH secretion decreases, only the dominant follicle(s) survive. Recently, some microRNAs (miR), such as miR-144, miR-202 and miR-873, have been found to be upregulated in healthy follicles in comparison with atretic ones [61]. The mature (Graafian) follicle has reached the max size (in humans > 10 mm), and the enclosed GV-stage oocytes has reached its final diameter (about 120 µm for humans).…”

Section: Folliculogenesismentioning

confidence: 99%

Technologies for the Production of Fertilizable Mammalian Oocytes

et al. 2019

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Women affected by ovarian pathologies or with cancer can usually preserve fertility by egg/embryo freezing. When oocyte retrieval is not feasible, the only option available is ovarian tissue cryopreservation and transplantation. The culture of follicles isolated from fresh or cryopreserved ovaries is considered still experimental, although this procedure is considered safer, because the risk of unintentional spreading of cancer cells eventually present in cryopreserved tissue is avoided. Animal and human small follicles can be cultured in vitro, but standardized protocols able to produce in vitro grown oocytes with the same developmental capacity of in vivo grown oocytes are not available yet. In fact, the different sizes of follicles and oocytes, the hormonal differences existing between mono- (e.g., human, goat, cow, and sheep) and poly-ovulatory (rodents and pig) species, and the incomplete identification of the mechanisms regulating the oocyte–follicle and follicle–ovary interrelationships affect the outcome of in vitro culture. From all these attempts, however, new ideas arise, and the goal of assuring the preservation of female reproductive potential appears a more realistic possibility. This review surveys and discusses advances and challenges of these technologies that, starting from a simple attempt, are now approaching the biosynthesis of a functional engineered ovary.

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Section: Folliculogenesismentioning

confidence: 99%

Technologies for the Production of Fertilizable Mammalian Oocytes

et al. 2019

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“…The miRNAs regulate about 30% of protein-coding genes in mammals since the different miRNAs may target the same mRNAs (5). Nucleotides sizes of miRNAs are differentially reported in the mammals, including 19-22 nucleotides (6), 20-24 nucleotides (6, 7), 21-23 nucleotide (4), and even 21-26 molecules (8). Consequently, the precise numbers of nucleotides of miRNAs remain undetermined (9,10).…”

Section: Introductionmentioning

confidence: 99%

“…FGCs play a key role in nourishing oocytes through secreting growth factors and hormones and regulating development of oocytes (15). It has been well known that miRNAs exert the vital functions in FGCs apoptosis and follicular development (FD) (8,16,17). The functions of specific miRNAs are implicated in different aspects of FGCs processes of the mammals, such as proliferation (18), differentiation (19) and cumulus expansion (20).…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies aimed to determine the roles of miRNAs on the FD of mammals using various approaches, including conditional knockout of miRNA biogenesis genes, high-throughput sequencing technologies in various animal models. Nowadays, it has been well known that miRNAs exert a significant role in FD and oocyte development of mammals (8,21).…”

Section: Introductionmentioning

confidence: 99%

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MicroRNAs regulate granulosa cells apoptosis and follicular development — A review

Gong

Yang

Bai

et al. 2020

Asian-Australas J Anim Sci

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Objective: MicroRNAs (miRNAs) are the most abundant small RNAs. Approximately annotated miRNAs genes have been found to be differentially expressed in ovarian follicles during the follicular development. Many miRNAs exert their regulatory effects on the apoptosis of follicular granulosa cells (FGCs) and follicular development (FD). However, accurate roles and mechanism of miRNAs regulating apoptosis of FGCs remain undetermined. Methods: In this review, we summarized the regulatory role of each miRNA or miRNA cluster on FGCs apoptosis and FD on the bases of 41 academic articles retrieved from PubMed and web of science and other databases. Results: Total of 30 miRNAs and 4 miRNAs clusters in 41 articles were reviewed and summarized in the present article. 29 documents indicated explicitly that 24 miRNAs and miRNAs clusters in 29 articles promoted or induced FGCs apoptosis through their distinctive target genes. The remaining 10 miRNAs and miRNAs of 12 articles inhibited FGCs apoptosis. were reported in all articles. MiRNAs exerted modulation actions by at least 77 signal pathways during FGCs apoptosis and FD. Conclusion: We concluded that miRNAs or miRNAs clusters could modulate the apoptosis of GCs (including follicular GCs, mural GCs and cumulus cells) by targeting their specific genes. A great majority of miRNAs show promoting role on apoptosis of FGCs in mammals. But the accurate mechanism of miRNAs and miRNA clusters has not been well understood. It is extremely necessary to ascertain clearly the role and mechanism of each miRNA or miRNA cluster in the future. Understanding precise functions and mechanisms of miRNAs in FGCs apoptosis and FD will be beneficial for finding new diagnostic and treatment strategy or scheme for infertility and ovarian diseases in humans and animals.

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“…The literature concerning the epigenetic changes mediated by cigarette smoke in the female reproductive system is limited. Two recent reviews summarized the roles of miRNAs in mammalian reproduction (Reza et al, ; Tesfaye et al, ), and experimental investigations characterized miRNAs patterns in both ovaries and oocytes (Imbar & Eisenberg, ; Toloubeydokhti, Bukulmez, & Chegini, ). When considering the roles of miRNAs in early embryo development, it is appropriate to note that sperm‐borne miRNAs do not contribute significantly to miRNAs in the zygote (Tang et al, ), thus suggesting a primary involvement of oocyte miRNAs in early embryonic development and survival.…”

Section: Introductionmentioning

confidence: 99%

Whole‐body exposure to cigarette smoke alters oocyte miRNAs expression in C57BL/6 mice

Budani

D’Aurora

Stuppia

et al. 2019

Molecular Reproduction Devel

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Cigarette smoke is toxic for the female reproductive system with particular reference to the ovary. The purpose of the study was to investigate if the microRNAs (miRNAs) pattern could be altered by cigarette smoke exposure in mouse oocytes. For this purpose, C57BL/6 mice were whole‐body exposed to three cigarettes daily, 7 days/week, for 2 or 4 months by a specific rodent ventilator. Mice were then superovulated and oocytes collected. MII oocytes pools obtained by single animals were deprived of cumulus cells and used to extract total RNA including miRNAs. TaqMan™ Rodent MicroRNA A Array v2.0 was used to analyze the miRNAs expression profile. The biological functions and the functional networks of the identified up‐ and downregulated miRNAs were analyzed by ingenuity pathway analysis software. The gene expression of deregulated‐miRNAs targets was evaluated. For the first time, the global miRNAs changes in mouse oocyte in response to cigarette smoke exposure were disclosed. Our results revealed significant modulation of miRNAs mainly involved in inflammatory processes, cellular proliferation, and apoptosis. miRNAs expression was altered in a time‐dependent manner. Smoke exposure induced an early downregulation of Dicer1. Transcriptional alterations of the modulated miRNAs major targets, estrogen receptor 1, peroxisome proliferator‐activated receptor‐alpha, and tumor protein 53, as well as that of other key regulatory genes, were evidenced. Cigarette smoke represents a stimulus able to alter miRNAs pattern in mouse oocyte. This study increases our understanding of the ovarian toxicity profile of cigarette smoke, and open new roads toward the identification of biomarkers of oocyte toxicity and dysregulation.

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MicroRNAs: tiny molecules with a significant role in mammalian follicular and oocyte development

Abstract: 25The genetic regulation of female fertility (follicular development, oocyte maturation,

Cited by 81 publications

References 174 publications

Technologies for the Production of Fertilizable Mammalian Oocytes

Technologies for the Production of Fertilizable Mammalian Oocytes

MicroRNAs regulate granulosa cells apoptosis and follicular development — A review

Whole‐body exposure to cigarette smoke alters oocyte miRNAs expression in C57BL/6 mice

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