Microsatellir.es genetic markers for white shrimp (<i>Penaeus schmitti</i>)

Espinosa, Georgina; Jager, Muriel; Borrell, Yaisel J.; García, Ernest; Corona, N.; Robainas, A.; Deutsch, Jean S.

doi:10.1042/bst028a185

Cited by 3 publications

(4 citation statements)

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“…Those projects need to establish the genetic diversity found among natural populations to plan crosses between these lineages (Da Silva 2007). Due to the great importance that P. schmitti farming had in Cuba, studies on the genetic variability of natural populations of this species were carried out with morphological techniques, allozymes, and microsatellites, targeting the improvement of captive broodstocks (Espinosa et al 2001, Borrell et al 2003. In Venezuela, García-Pinto (1970) studied morphometric characters of juveniles and adults of this species captured in Lake Maracaibo and the Gulf of Venezuela.…”

Section: Introductionmentioning

confidence: 99%

Morphological variations of southern white shrimp Penaeus schmitti (Burkenroad, 1936) (Crustacea: Dendrobranchiata: Penaeidae) in natural populations of Cuba and Brazil

Alaiza¹,

Silva²,

Peixoto³

et al. 2022

Lat. Am. J. Aquat. Res.

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The objective of this study was to identify morphological characteristics of different populations of Penaeus schmitti Burkenroad, 1936 to contribute to its biology and conservation. In order to compare geographically isolated populations, between 2014 and 2016, selected biological variables were analyzed in six populations dispersed along the Brazilian coast and one from the Cuban coast. Specimens with eight upper rostral teeth predominated. The ordination of shrimp measurements by non-metric multidimensional scaling showed the greatest dispersion on data from Cuba, Rio Grande do Norte, and Espirito Santo, which are also the localities that presented the highest coefficient of variation. On the other hand, the values corresponding to Sergipe, Rio de Janeiro, Paraná, and Santa Catarina showed a greater similarity or clustering of the data of each location. Tail weight was estimated using length and depth of the sixth abdominal segment. The populations of Santa Catarina, Brazil (28°S), had the longest and heavier abdomens proportionally among all the compared populations, while the organisms of Manzanillo, Cuba (20°N), had proportionally shorter abdomens and the narrowest cephalothorax. The regression equation indicated a positive allometric growth for P. schmitti, and the largest total length reported for genus Penaeus in the natural environment corresponded to a P. schmitti specimen. This information could be useful in future taxonomic studies to correctly identify the species and its morphological variability. Color differences were also observed, with white ventral spots in the sternite XIV of some females from Brazilian populations, which was not previously reported in the literature. Extending these studies is recommended to contribute to the conservation and culture of P. schmitti.

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Section: Introductionmentioning

confidence: 99%

Morphological variations of southern white shrimp Penaeus schmitti (Burkenroad, 1936) (Crustacea: Dendrobranchiata: Penaeidae) in natural populations of Cuba and Brazil

Alaiza¹,

Silva²,

Peixoto³

et al. 2022

Lat. Am. J. Aquat. Res.

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“…Estos son cadenas de ADN de secuencias simples repetidas, de longitud variable y que se distribuyen uniformemente en regiones no codificantes del genoma (Goldstein y Schlötterer, 1999). Para L. schmitti solo existen cinco loci microsatélites registrados en GenBank, con los cuales se ha estudiado la estructura poblacional a diferentes escalas geográficas (Espinosa et al, 2001;Maggioni et al, 2003;Borrell et al, 2004;Luvesuto, 2006). En el caso de L. occidentalis no existe información genética disponible.…”

Section: Introductionunclassified

Amplificación Cruzada De Microsatélites Para Estudios Poblacionales De Dos Especies De Camarón Del Género Litopenaeus en Colombia

Fresneda-Rodríguez

Chasqui-Velasco

Alonso-Carvajal

et al. 2016

BIM

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Microsatellites are molecular markers frequently used in population genetic studies despite of the high cost, and long time involved in developing them, mainly due to their high specificity. One method to save money and time is cross-amplification, which is the DNA amplification of the target species using primers developed for a different species. By using cross-amplification, the suitability of 15 developed microsatellite loci from Litopenaeus setiferus and L. vannamei to amplify microsatellite regions of L. schmitti and L. occidentalis was evaluated. Five primers showed consistent amplification and were polymorphic in L. schmitti and four in L. occidentalis. These results point out the usefulness of cross-amplification with these primers for population genetics studies of both species.

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“…Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed: Farfantepenaeus notialis (Robainas et al, 2002;Robainas et al, 2003), Farfantepenaeus paulensis and Farfantepenaeus subtilis (Maggioni and Rogers 2002), Fenneropenaeus chinensis (Wang et al, 2005), Fenneropenaeus merguiensis (Wanna et al, 2004), Litopenaeus schmitti (Espinosa et al, 2001;Maggioni et al, 2003;Borrel et al, 2004), Litopenaeus setiferus (Ball et al, 1998), Litopenaeus stylirostris (Vonau et al, 1999;Bierne et al, 2000), Litopenaeus vannamei (Wolfus et al, 1997;Cruz et al, 2002;Meehan et al, 2003;Pérez et al, 2005), Marsupenaeus japonicus (Moore et al, 1999), Penaeus esculentus (Meadows et al, 2003) and Penaeus monodon (Xu et al, 1999;Pongsomboon et al, 2000;Brooker et al, 2000;Xu et al, 2001;Wuthisuthimethavee et al, 2003;Pan et al, 2004). Cross-species amplification results are practically inexistent, being limited to a few loci described for L. vannamei that were also efficient in Rimapenaeus byrdi (= Trachypenaeus byrdi) and L. stylirostris (Pérez et al 2005).…”

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confidence: 99%

“…; (7) number and size of alleles per locus; and (8) results generated by the TRF program during the analysis, including any inconsistencies with the literature concerning the locus in question. Among the main observed inconsistencies in the sequences analyzed were the presence of microsatellites which were not flanked by one or both of the described primers, partially or totally absent repeats in the sequence described, microsatellites described with different motifs than those found by the TRF program and microsatellites with more than one GenBank ID or for which the ID numbers were different to those described in the paper in which it was cited.Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed: Farfantepenaeus notialis (Robainas et al, 2002;Robainas et al, 2003) (Espinosa et al, 2001;Maggioni et al, 2003; Borrel et al, 2004), Litopenaeus setiferus (Ball et al, 1998), Litopenaeus stylirostris (Vonau et al, 1999;Bierne et al, 2000), Litopenaeus vannamei (Wolfus et al, 1997;Cruz et al, 2002;Meehan et al, 2003;Pérez et al, 2005), Marsupenaeus japonicus (Moore et al, 1999), Penaeus esculentus (Meadows et al, 2003) and Penaeus monodon (Xu et al, 1999;Pongsomboon et al, 2000;Brooker et al, 2000;Xu et al, 2001;Wuthisuthimethavee et al, 2003;Pan et al, 2004). Cross-species amplification results are practically inexistent, being limited to a few loci described for L. vannamei that were also efficient in Rimapenaeus byrdi (= Trachypenaeus byrdi) and L. stylirostris (Pérez et al 2005).…”

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confidence: 99%

In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database

et al. 2007

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We performed an in silico analysis of all microsatellites so far described for penaeid shrimp and for which the polymorphic behavior has previously been analyzed. The objective of the study was to evaluate the structural characteristics of these microsatellites and identifying patterns which allow the characterization of the nature of these sequences in the penaeid genome. All data were compiled in a free-access database specially constructed for this study. Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed and simple and compound microsatellites with di-, tri-, tetra-, penta-and hexanucleotide motifs were found. Dinucleotides and trinucleotides were the most frequent motifs among both the simple and the compound microsatellites. Although a certain bias related to different microsatellite isolation methodology could not be discarded, it is possible that part of this microsatellite abundance reflects some degree of conservation of microsatellite motifs among the different species. There was a pronounced motif variability within and between species, indicating high differentiation dynamism of these repetitions in this animal group. This study not only sheds light on the structure of the microsatellites present in the penaeid shrimp genome but also resulted in the free-access Penaeid Shrimp Microsatellite Database (available at http://www.shrimp.ufscar.br) which may be very useful for optimizing the use of these microsatellites.Key words: Crustacea, microsatellite structure, Penaeidae, SSR.Received: September 19, 2006; Accepted: May 7, 2007. Over the last few decades, microsatellite sequences have been widely used in many animal groups, including shrimp, and principally in economically important species (Wolfus et al., 1997;Bierne et al., 2000;Xu et al., 2001). Due to their high level of allelic variation and codominant nature, microsatellite loci have been extremely useful for the determination of genetic diversity, stock discrimination, identification of lineages and individuals, the establishment of pedigrees, the development of breeding programs, linkage map studies and the identification of loci related to commercially important characteristics (Moore et al., 1999;Ozaki et al., 2000).In the past half decade, different research groups have been striving to characterize microsatellite markers in the genome of a number of penaeid shrimp species (Cruz et al., 2002;Meehan et al., 2003;Pérez et al., 2005). However, the validation of these sequences has generally not been easy. The penaeid genome seems to contain very long microsatellite repetitions, hindering the cloning and sequencing of microsatellites containing both flanking regions. A few comparisons demonstrate that the microsatellites found in penaeids (Tassanakajon et al., 1998;Cruz et al., 2002) are approximately twice the size of the sequences found in other animal groups (Estoup et al., 1993;Brooker et al., 1994), being four or five times larger in so...

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Microsatellir.es genetic markers for white shrimp (Penaeus schmitti)

Abstract: Vertebrate blood coagulation is a delicately balanced process whose central feature is the conversion of fibrinogen to fibrin by the protease thrombin. The plasma coagulation factors, factor VII

Cited by 3 publications

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Morphological variations of southern white shrimp Penaeus schmitti (Burkenroad, 1936) (Crustacea: Dendrobranchiata: Penaeidae) in natural populations of Cuba and Brazil

Morphological variations of southern white shrimp Penaeus schmitti (Burkenroad, 1936) (Crustacea: Dendrobranchiata: Penaeidae) in natural populations of Cuba and Brazil

Amplificación Cruzada De Microsatélites Para Estudios Poblacionales De Dos Especies De Camarón Del Género Litopenaeus en Colombia

In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database

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