Microsatellite Alterations on Chromosome 8 of Hepatocellular Carcinoma

Zhang, Shu‐Hui; Xian, Zhi‐Hong; Wu, Mengchao

doi:10.1007/s10330-004-0162-y

Cited by 4 publications

(6 citation statements)

References 19 publications

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“…However, a search for relationships between the specific regions of deletion and clinical parameters showed a significant association of loss of the 4q34-35 region with alcohol intake (p=0.005) and with high grade of differentiation (p=0.02). The study results were inconsistent with those of Bando et al (31) and Zhang et al (32).…”

Section: Discussioncontrasting

confidence: 57%

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Analysis of loss of heterozygosity on chromosome 4q in hepatocellular carcinoma using high-throughput SNP array

Xia¹

2009

Oncol Rep

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Abstract. To identify tumour suppressor genes (TSGs) associated with hepatocellular carcinoma (HCC) on chromosome 4q using a high-throughput single nucleotide polymorphism (SNP) array, we first scanned for loss of heterozygosity (LOH) of 40 SNPs on chromosome 4q and discovered 2 hot regions: 4q24-26 and 4q34.3-35. We then further scanned for LOH of 338 SNPs in genes around 4q34.3-35 and discovered 3 genes with the most frequent LOH: nei endonuclease VIIIlike 3 (NEIL3), interferon regulatory factor 2 (IRF2) and inhibitor of growth family member 2 (ING2). A review of the literature indicates only ING2 might be a TSG associated with HCC. IntroductionPrimary liver cancer, which consists predominantly of hepatocellular carcinoma (HCC), has risen to become the fifth most common malignancy worldwide and the third leading cause of cancer mortality (1). Previous studies have demonstrated that accumulation of the activation of oncogenes and/or inactivation of tumour suppressor genes (TSGs) is involved in the carcinogenesis of human cancer (2-4). However, the inactivation of TSGs was especially reported to play a more critical role in HCC pathogenesis (5). Recently, loss of heterozygosity (LOH) as an indirect procedure to identify TSGs has been widely used. Frequent allele deletions are observed on chromosomes 1p, 4q, 5q, 8p, 8q, 9p, 10q, 11p, 13q, 14q, 16q, and 17p in HCC (6-13).Whole-genome scans and chromosome analyses have been performed, and the chromosome 4q carries deletions in HCC more frequently than in other tumours (14)(15)(16)(17)(18)(19)(20)(21)(22). In early studies, we investigated LOH on 22 autosomes with 382 sets of microsatellite markers (MS) in 65 cases of HCC. The results showed that the frequency of LOH on 4q was 48.1% and that TSGs associated with HCC might be on 4q.In the present study, we first scanned chromosome 4q with a high-throughput SNP array to analyse the LOH and found the region with the most frequent LOH, then further scanned the SNPs around the region to search for the TSGs associated with HCC. Materials and methodsPatients and tissue specimens. All matched primary hepatocellular carcinoma tissue and adjacent cancer-free tissue specimens (n=69) were obtained from those patients who underwent surgical resection of their diseases in the Sun Yat-sen University Cancer Center between 2005 and 2007. The patients who received any preoperative treatment such as chemotherapy and radiotherapy were excluded. The 69 patients included 60 males and 9 females with a median age of 52 years (range, 21-75 years). The fresh tissues were immediately immersed in RNAlater (Ambion, Inc., USA) after surgical resection, stored at 4˚C overnight to allow thorough penetration of the tissues, then frozen at -80˚C until RNA and DNA extraction. Both cancer and corresponding adjacent cancer-free tissues not less than 2 cm away from the liver were sampled from cancer patients and verified by pathological examination.DNA extraction. Both cancer and corresponding cancer-free liver tissues were digested with proteinase K (1...

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Section: Discussioncontrasting

confidence: 57%

“…Zhang et al (32) detected LOH on chromosome 4q using PCR-simple sequence length polymorphism (PCR-SSLP) on 10 high-polymorphic microsatellite markers in 56 HCC and found that LOH on D4S426 (4q35), D4S1615 (4q31.1) and D4S1652 (4q34-qter) was more frequent in poorly or moderately differentiated HCC than in well-differentiated HCC.…”

Section: Discussionmentioning

confidence: 99%

Analysis of loss of heterozygosity on chromosome 4q in hepatocellular carcinoma using high-throughput SNP array

Xia¹

2009

Oncol Rep

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“…In clinical settings, however, microsatellite analysis may not be suitable as a standard method for differentiating between IM and MC in view of its labor intensiveness and high cost. Therefore, 13 microsatellite markers, previously proven to be representative markers of LOH and MSI were carefully selected (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). First, 9 microsatellite markers were analyzed, but DDx was achieved in only 71 cases (88.9%).…”

Section: Discussionmentioning

confidence: 99%

“…Microsatellite analysis. We examined 13 chromosomal microsatellite loci (D1S233, D1S407, D3S1539, D8S277, D8S261, D9S156, D9S254, D10S520, D13S284, D16S515, D4S1554, D17S513, and TP53) in 237 specimens (non-cancerous liver tissue, carcinoma tissue from the first hepatectomy, and carcinoma tissue from the re-hepatectomy) from 79 patients to detect loss of heterozygosity (LOH) on the chromosome arms using relatively frequent HCC loci that had been described in previous reports (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). The PCR primers were synthesised based on the genome sequences in a genome database.…”

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Microsatellite Analysis of Recurrent Lesions Confirms Merit of Anatomical Liver Resection for Hepatocellular Carcinoma

et al. 2019

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Background/Aim: This study aimed to obtain accurate differential diagnosis (DDx) of multicentric carcinogenesis (MC) and intrahepatic metastasis (IM) in recurrent lesions of hepatocellular carcinoma. Materials and Methods: A total of 79 patients who underwent rehepatectomy (2000-2013) were examined. PCR was used to analyze 13 chromosomal microsatellite loci by PCR. On the basis of this genetic analysis, the recurrent lesions were diagnosed as IM, MC or not determined (ND). Subsequently, DDx was compared with types of resection and outcome. Results: The recurrent lesions were diagnosed as IM in 33 patients, MC in 44, and ND in 2. The anatomical resection group included 14 IM lesions (28%) and 36 MC lesions (72%), while the non-anatomical resection group included 19 IM lesions (70%) and 8 MC lesions (30%) (p<0.001). Conclusion: Anatomical resection at initial hepatectomy may reduce the likelihood of IM recurrence, leading to a better outcome for patients with HCC. Patients and MethodsPatients. The retrospective design of this study (0054629) was reviewed and approved by the institutional review board. Between April 2000 and October 2013, 537 patients with primary HCC underwent anatomical or non-anatomical resection for the first time at our department. After excluding cases involving multiple recurrent lesions and relapse-free patients, a total of 79 patients with 4315

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“…Liver cancer is not sensitive to chemotherapy and radiotherapy. For patients with early liver cancer of Ia-IIa, therapeutic resection of liver cancer is currently the preferred treatment option, 3 but the recurrence and metastasis rate after 5 years is as high as 40% to 70%. 4 Aspirin, also known as acetylsalicylic acid, has been used clinically for > 100 years.…”

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confidence: 99%

Aspirin Use and the Risk of Hepatocellular Carcinoma

Wang

Liu

et al. 2022

Journal of Clinical Gastroenterology

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Introduction and Aim: The use of aspirin is a potential protective factor against the development of hepatocellular carcinoma (HCC). Therefore, we conducted a meta-analysis to evaluate the contribution of aspirin to the risk of HCC. Methods:We searched for PubMed and EMBASE through September 2021.Results: Eighteen studies (16 cohort, 2 case-control) were included. Aspirin users were less likely to develop HCC than nonusers [adjusted odds ratio (OR), 0.54; 95% confidence interval (CI): 0.44-0.66]. Stratified analysis showed that aspirin reduced the risk of HCC in Asian and Western populations (OR, 0.59 vs. 0.67). Besides, aspirin has protective effects against HCC after hepatitis B virus (OR, 0.70; 95% CI: 0.52-0.93) and hepatitis C virus infections (OR, 0.41; 95% CI: 0.23-0.73). Aspirin has protective effects on people with chronic liver disease (OR, 0.46; 95% CI: 0.31-0.67) and on the general population (OR, 0.65; 95% CI: 0.54-0.79). In addition, confounding factors have an important impact on the results of aspirin prevention of liver cancer before (OR, 0.28; 95% CI: 0.06-1.27) and after (OR, 0.58; 95% CI: 0.47-0.71) adjustment. Further studies have shown that those in the long duration group do not experience better effects in preventing HCC (OR, 0.62 vs. 0.63). A further meta-analysis of 3 articles showed that the use of aspirin did not increase the risk of bleeding in patients with HCC (OR, 1.19; 95% CI: 0.87-1.64). Conclusion:Our meta-analysis shows that the use of aspirin is associated with a lower risk of liver cancer.

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Microsatellite Alterations on Chromosome 8 of Hepatocellular Carcinoma

Cited by 4 publications

References 19 publications

Analysis of loss of heterozygosity on chromosome 4q in hepatocellular carcinoma using high-throughput SNP array

Analysis of loss of heterozygosity on chromosome 4q in hepatocellular carcinoma using high-throughput SNP array

Microsatellite Analysis of Recurrent Lesions Confirms Merit of Anatomical Liver Resection for Hepatocellular Carcinoma

Aspirin Use and the Risk of Hepatocellular Carcinoma

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