Microsatellite Analysis on Genetic Variation in Two Populations of Red Mangrove Rhizophora Mangle L. (Rhizophoraceae) and Its Implication to Conservation

Basyuni, Mohammad; Baba, Shigeyuki; Oku, Hirosuke

doi:10.1088/1757-899x/180/1/012243

Cited by 15 publications

(12 citation statements)

References 8 publications

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“…et al and Kennedy et al found low outcrossing rates, suggesting that a high rate of selfing decreased the genetic diversity. Evidence of low genetic diversity has been detected by previous studies on mangrove species (Basyuni et al 2017;Pil et al, 2011). The effect of the mating system on genetic diversity has been observed in other studies on tropical tree species (Tambarussi et al, 2016).…”

Section: Discussionsupporting

confidence: 53%

“…This fact can be explained by the process of forest fragmentation. Other studies have shown similar levels of genetic diversity with only five (Basyuni et al, 2017) and seven (Kennedy and Garavelli, 2017) microsatellite loci. The four microsatellite markers in this study presented a high power of parent exclusion, moderate genetic diversity and no linkage disequilibrium.…”

Section: Discussionmentioning

confidence: 65%

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GENETIC DIVERSITY AND MATING SYSTEM OF Rhizophora mangle L. (RHIZOPHORACEAE) IN NORTHERN BRAZIL REVEALED BY MICROSATELLITE ANALYSIS

Francisco

Tambarussi²,

Alves

et al. 2018

CERNE

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Genetic diversity and mating system of Rhizophora mangle L. (Rhizophoraceae) in Northern Brazil revealed by microsatellite analysis. CERNE, v. 24, n. 4, p. 295-302, 2018. HIGHLIGHTS Rhizophora mangle has low occurrence of self-pollination and low occurrence of mating among relatives. One to 12 pollen donors fertilized each seed tree. However, Genetic diversity was low. The number of seed trees required for seed collection (m) was 62 and ranged from 40 to 107. This study provides ecological and management implications for R. mangle.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 65%

GENETIC DIVERSITY AND MATING SYSTEM OF Rhizophora mangle L. (RHIZOPHORACEAE) IN NORTHERN BRAZIL REVEALED BY MICROSATELLITE ANALYSIS

Francisco

Tambarussi²,

Alves

et al. 2018

CERNE

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“…Ganoderma boninense mycelium, which has been grown on the PDA media, were used for DNA extraction. Total DNA was extracted from B. boninense using the CTAB method, as previously described with minor modification (Basyuni et al 2017). The DNA quality was tested based on UV-illuminator and documented using the Gel doc.…”

Section: The Dna Extractionmentioning

confidence: 99%

Genetic diversity and structure of Ganoderma boninense isolates from oil palm and other plantation crops

et al. 2020

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Abstract. Purba A, Hayati R, Putri LAP, Chalil D, Afandi D, Syahputra I, Basyuni M. 2020. Genetic diversity and structure of Ganoderma boninense isolates from oil palm and other plantation crops. Biodiversitas 21: 451-456. Oil palm is an economically important plant, which one of the most important sources of vegetable oil in the world. However, oil palm plantation and other crops face the treat basal stem rot (BSR) disease by Ganoderma boninense. A study on genetic diversity and structure of G. boninense is therefore needed in order to formulate improved control strategies for this disease. This work aimed to analyze the genetic diversity and structure of the G. boninense isolates derived from different hosts, 131 oil palm (Elaeis guineensis), six rubber (Hevea brasiliensis), three coconuts (Cocos nucifera), and three lontar palm (Borassus flabellifer). Genetic diversity and population structure of G. boninense isolates were investigated using six SSR markers with GenAlex 6.502 software. Results showed that several microsatellite loci indicated specific primary success rates, such as KT124402, KT124399, and KT124394, depicting high polymorphism content (>75%). This result suggested that these markers were equally effective in determining the polymorphisms of G. boninense isolates. A hierarchical analysis of molecular variance (AMOVA) revealed that genetic diversity mostly found among individuals within a population (88%), then among populations (8%), and within individuals (4%).. Phylogeny analysis showed two clusters of Ganoderma isolates. which was considered variation as dissimilar across with origin. The present study indicated that G. boninense from oil palm was predominantly comprised of a genetically distinct individual.

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“…Total DNA was extracted from the propagules as progeny arrays using modified CTAB procedure [6]. Five primer pairs, MSBgy025, MSBgy027, MSBgy030, MSBgy031 and MSBgy188 [7] were used in B. gymnorrhiza as well as five primer pairs (Kcan004, Kcan005, Kcan009, Kcan011, and Kcan034) of K. obovata according to Sugaya et al [8].…”

Section: Dna Extraction and Microsatellite Analysismentioning

confidence: 99%

Microsatellite analysis of genetic diversity and structure of Bruguiera gymnorrhiza and Kandelia obovate

2018

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Microsatellite loci were used for estimating genetic diversity and structure for three populations of B. gymnorrhiza and K. obovata (Rhizophoracea) in Okinawa, Japan. Thirty propagules of individual samples representing the population of both species were genotyped at five microsatellites. The level of observed heterozygosity (HO) was observed for several population, overall loci, ranged 0.422-0.800 with an average 0.627 for B. gymnorrhiza and 0.477-0.822 with an average 0.665 for K. obovata, indicating both species had relatively low genetic diversity. Both species showed low levels of allelic diversity, 3-5 and 3-5 alleles per locus, respectively. Gene diversitywas also maintained within populations (HS: 0.741 and 0.954). Additionally, an analysis of molecular variance (AMOVA) based on the immeasurable alleles model (F-statistics), for B. gymnorrhiza and K. obovata found that most of the variation resided within individuals in the total populations, i.e. 79.78 % and 69.90 % respectively, and among individuals within populations, i.e.14.30 % and 27.95 % respectively. There was little variation between populations, i.e. 5.92 % and 2.15 % for B. gymnorrhiza and K. obovata, respectively. The high-level genetic differentiation within individuals and populations both species may be due to the geographic range of the species, mating system, and environmental factors.

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Microsatellite Analysis on Genetic Variation in Two Populations of Red Mangrove Rhizophora Mangle L. (Rhizophoraceae) and Its Implication to Conservation

Cited by 15 publications

References 8 publications

GENETIC DIVERSITY AND MATING SYSTEM OF Rhizophora mangle L. (RHIZOPHORACEAE) IN NORTHERN BRAZIL REVEALED BY MICROSATELLITE ANALYSIS

GENETIC DIVERSITY AND MATING SYSTEM OF Rhizophora mangle L. (RHIZOPHORACEAE) IN NORTHERN BRAZIL REVEALED BY MICROSATELLITE ANALYSIS

Genetic diversity and structure of Ganoderma boninense isolates from oil palm and other plantation crops

Microsatellite analysis of genetic diversity and structure of Bruguiera gymnorrhiza and Kandelia obovate

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