Microsatellite analysis reveals genetic structure of Leishmania tropica

Schwenkenbecher, Jan M.; Wirth, Thierry; Schnur, Lionel F.; Jaffe, Charles L.; Schallig, Henk D. F. H.; Al-Jawabreh, Amer; Hamarsheh, Omar; Azmi, Kifaya; Pratlong, Francine; Schönian, Gabriele

doi:10.1016/j.ijpara.2005.09.010

Cited by 128 publications

(146 citation statements)

References 48 publications

Supporting

Mentioning

135

Contrasting

Order By: Relevance

“…Moreover, a strong population structure was found at a micro-geographical scale as the populations within the different countries were genetically heterogenous. This is in accord with the results of earlier population studies of L. infantum Seridi et al 2008;Amro et al 2009;Chargui et al 2009), L. tropica (Schwenkenbecher et al 2006), and L. major . Recent research by MLMT on Sudanese L. donovani showed populations consistent with inbreeding, although it also detected the first population without departure from Hardy-Weinberg equilibrium (Baleela, personal communication).…”

supporting

confidence: 92%

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

2010

View full text Add to dashboard Cite

S U M M A R YMolecular approaches are being used increasingly for epidemiological studies of visceral and cutaneous leishmaniases. Several molecular markers resolving genetic differences between Leishmania parasites at species and strain levels have been developed to address key epidemiological and population genetic questions. The current gold standard, multilocus enzyme typing (MLEE), needs cultured parasites and lacks discriminatory power. PCR assays identifying species directly with clinical samples have proven useful in numerous field studies. Multilocus sequence typing (MLST) is potentially the most powerful phylogenetic approach and will, most probably, replace MLEE in the future. Multilocus microsatellite typing (MLMT) is able to discriminate below the zymodeme level and seems to be the best candidate for becoming the gold standard for distinction of strains. Population genetic studies by MLMT revealed geographical and hierarchic population structure in L. tropica, L. major and the L. donovani complex. The existence of hybrids and gene flow between Leishmania populations suggests that sexual recombination is more frequent than previously thought. However, typing and analytical tools need to be further improved. Accessible databases should be created and sustained for integrating data obtained by different researchers. This would allow for global analyses and help to avoid biases in analyses due to small sample sizes.

show abstract

supporting

confidence: 92%

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

2010

View full text Add to dashboard Cite

show abstract

“…Within L. killicki as well as non-killicki strains of L. tropica, various genetic subdivisions (RD patterns) are apparent (Chaara et al, 2015a,b). In Asia, the population structure of L. tropica has remained stable for 55 years (Schwenkenbecher et al, 2006).…”

Section: Parasitic Protozoamentioning

confidence: 99%

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Tibayrenc

Ayala

2017

Advances in Parasitology

View full text Add to dashboard Cite

“…Multilocus microsatellite typing (MLMT) has proven highly discriminatory in the typing of strains (5) and has been successfully used in population genetic studies of different Old World species of Leishmania (2,19,20,32). This approach could be performed directly with clinical materials without prior cultivation of the parasites.…”

mentioning

confidence: 99%

Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania ( Viannia ) Species

et al. 2009

View full text Add to dashboard Cite

A multilocus microsatellite typing (MLMT) approach based on the analysis of 15 independent loci has been developed for the discrimination of strains belonging to different Viannia species. Thirteen microsatellite loci were isolated de novo from microsatellite-enriched libraries for both Leishmania braziliensis and L. guyanensis. Two previously identified markers, AC01 and AC16, were modified and added to our marker set. Cutaneous leishmaniasis (CL) is a serious but neglected public health problem that is endemic in 22 countries of Latin America, with Brazil and Colombia being among the six countries reporting 90% of the worldwide cases. Species of the subgenus Viannia cause the majority of cases of CL and mucocutaneous leishmaniasis (MCL) in South America and often overlap in their distribution (22). The subgenus is subdivided into species complexes representing Leishmania braziliensis, L. guyanensis, and L. naiffi (8). The L. guyanensis complex includes some named species, such as L. panamensis and L. shawi, which were found to be very similar to each other (7).

show abstract

Microsatellite analysis reveals genetic structure of Leishmania tropica

Cited by 128 publications

References 48 publications

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

Molecular approaches for a better understanding of the epidemiology and population genetics ofLeishmania

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Development of a Multilocus Microsatellite Typing Approach for Discriminating Strains of Leishmania ( Viannia ) Species

Contact Info

Product

Resources

About