Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Némorin, Alice; David, Jacques; Maledon, Erick; Nudol, Elie; Dalon, J.; Arnau, Gemma

doi:10.1093/aob/mct145

Cited by 30 publications

(28 citation statements)

References 47 publications

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“…Most of the accessions included in our study are not fertile. Triploids are sterile [68, 69], as are several diploids and tetraploids. The results presented here allowed the identification of different groups of fertile cultivars with a good tolerance to anthracnose disease (Groups II, III, VI, XI, XVI and XVII) as well as several groups that contain cultivars known for the quality of their tubers: organoleptic, shape, dry matter content, and others (Groups III, VI, VII, VIII).…”

Section: Discussionmentioning

confidence: 99%

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Arnau

Bhattacharjee

et al. 2017

PLoS ONE

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Yams (Dioscorea sp.) are staple food crops for millions of people in tropical and subtropical regions. Dioscorea alata, also known as greater yam, is one of the major cultivated species and most widely distributed throughout the tropics. Despite its economic and cultural importance, very little is known about its origin, diversity and genetics. As a consequence, breeding efforts for resistance to its main disease, anthracnose, have been fairly limited. The objective of this study was to contribute to the understanding of D. alata genetic diversity by genotyping 384 accessions from different geographical regions (South Pacific, Asia, Africa and the Caribbean), using 24 microsatellite markers. Diversity structuration was assessed via Principal Coordinate Analysis, UPGMA analysis and the Bayesian approach implemented in STRUCTURE. Our results revealed the existence of a wide genetic diversity and a significant structuring associated with geographic origin, ploidy levels and morpho-agronomic characteristics. Seventeen major groups of genetically close cultivars have been identified, including eleven groups of diploid cultivars, four groups of triploids and two groups of tetraploids. STRUCTURE revealed the existence of six populations in the diploid genetic pool and a few admixed cultivars. These results will be very useful for rationalizing D. alata genetic resources in breeding programs across different regions and for improving germplasm conservation methods.

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Section: Discussionmentioning

confidence: 99%

Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers

Arnau

Bhattacharjee

et al. 2017

PLoS ONE

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“…In root and tuber crops, microsatellites have been utilized to determine the progeny-paternity relationship (Table 1). Microsatellites have been used for parentage analysis in yams [32][33][34][35][36][37][38][39], potatoes [44][45][46][48][49][50]52,53,55], cassava [63][64][65][66][67][68][69][70][71][72][73] and sweet potato [76][77][78][79][80][81][82]84,85].…”

Section: Molecular Marker Systemsmentioning

confidence: 99%

Can Parentage Analysis Facilitate Breeding Activities in Root and Tuber Crops?

et al. 2018

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Controlled pollination in root and tuber crops is challenging. Complex ploidy, cross-incompatibility, erratic flowering patterns, outcrossing, etc., limit the efficiency of breeding progress in these crops. Half-sib breeding that involves random pollination among parents is a viable method to harness genetic gain in outcrossing crops that are problematic for performing planned and controlled pollination. The authenticity of resulting progenies from the half-sib breeding is essential to monitor the selection gain in the breeding program. Parentage analysis facilitated by molecular markers is among the available handy tools for crop breeders to maximize genetic gain in a breeding program. It can help to resolve the identity of half-sib progenies and reconstruct the pedigree in the outcrossing crops. This paper reviews the potential benefits of parentage analysis in breeding selected outcrossing root and tuber crops. It assesses how paternity analysis facilitates breeding activities and the ways it improves genetic gain in the root and tuber breeding programs. Conscious use of complementary techniques in the root and tuber breeding programs can increase the selection gain by reducing the long breeding cycle and cost, as well as reliable exploitation of the heritable variation in the desired direction.

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“…Flow cytometry is one of the methods to evaluate genetic stability. Currently this is not sufficient way to determine ploidy levels in plants but this is a popular, fast and easy technique [37][38][39]. Flow cytometry is widely used to establish DNA content and genome size in micropropagated medicinal plants [40].…”

Section: Disscusionmentioning

confidence: 99%