Microsatellite-based association mapping for agronomic traits in mungbean (Vigna radiata L. Wilczek)

Singh, C. M.; Prajapati, Umashanker; Gupta, Sanjeev; Pratap, Aditya

doi:10.1007/s12041-021-01336-9

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…In this study, LG 6 and LG 7 each had one QTL for iron, but LG7 had three QTLs for zinc. On the LG 11 map, Singh ( 87 ) discovered a potential QTL (qFe-11-1) for iron. One QTL for iron was found at LG11 in this study.…”

Section: Discussionmentioning

confidence: 99%

Genome wide association analysis for grain micronutrients and anti-nutritional traits in mungbean [Vigna radiata (L.) R. Wilczek] using SNP markers

et al. 2023

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Mungbean is an important food grain legume for human nutrition and nutritional food due to its nutrient-dense seed, liked palatability, and high digestibility. However, anti-nutritional factors pose a significant risk to improving nutritional quality for bio-fortification. In the present study, genetic architecture of grain micronutrients (grain iron and zinc concentration) and anti-nutritional factors (grain phytic acid and tannin content) in association mapping panel of 145 diverse mungbean were evaluated. Based on all four parameters genotypes PUSA 1333 and IPM 02-19 were observed as desired genotypes as they had high grain iron and zinc concentration but low grain phytic acid and tannin content. The next generation sequencing (NGS)-based genotyping by sequencing (GBS) identified 14,447 genome-wide SNPs in a diverse selected panel of 127 mungbean genotypes. Population admixture analysis revealed the presence of four different ancestries among the genotypes and LD decay of ∼57.6 kb kb physical distance was noted in mungbean chromosomes. Association mapping analysis revealed that a total of 20 significant SNPs were shared by both GLM and Blink models associated with grain micronutrient and anti-nutritional factor traits, with Blink model identifying 35 putative SNPs. Further, this study identified the 185 putative candidate genes. Including potential candidate genes Vradi07g30190, Vradi01g09630, and Vradi09g05450 were found to be associated with grain iron concentration, Vradi10g04830 with grain zinc concentration, Vradi08g09870 and Vradi01g11110 with grain phytic acid content and Vradi04g11580 and Vradi06g15090 with grain tannin content. Moreover, two genes Vradi07g15310 and Vradi09g05480 showed significant variation in protein structure between native and mutated versions. The identified SNPs and candidate genes are potential powerful tools to provide the essential information for genetic studies and marker-assisted breeding program for nutritional improvement in mungbean.

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Section: Discussionmentioning

confidence: 99%

Genome wide association analysis for grain micronutrients and anti-nutritional traits in mungbean [Vigna radiata (L.) R. Wilczek] using SNP markers

et al. 2023

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“…In our previous report, we found prevalence of MYMIV detected and validated at molecular level in YMD development in central zone (Singh, Pratap, et al, 2020). Islam et al (2012), Singh, Gupta, et al (2021) suggested the use of species specific to primers MYMIV ‐A and MYMIV ‐B for detecting both DNA‐A and DNA‐B components of MYMIV . The artificial screening technique for precise screening of breeding materials against the specific virus in mungbean and urdbean have also been adopted by various workers like Gupta et al (2013), Sudha et al (2013).…”

Section: Discussionmentioning

confidence: 99%

“…In this situation, identification and characterization of resistant genes from different genetic stocks will be the most effective approach for improving the resistance. Therefore, identification of donors from different Vigna species including exotic germplasm and even wild relatives will be more effective for improving the durability of resistance in cultivated types (Singh, Gupta, et al, 2021). In the present study, we phenotyped a comprehensive panel of urdbean for MYMIV resistance.…”

Section: Discussionmentioning

confidence: 99%

“…This indicated the possibility of inclusion of molecular markers in accelerating molecular breeding programme. Singh, Gupta, et al (2021) also screened a panel of 290 genotypes of Vigna belonging to 19 different species in six different environment for MYMIV resistance and found 70 HR accessions. They also utilized the 90 molecular markers for association mapping.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of both DNA components together are required for the development of YMD. The wider host range choice of these viruses make the pathogen available throughout the year (Mishra et al, 2020; Singh et al, 2019; Singh, Gupta, et al, 2021) also gives the chance for pathogen co‐evolution. Although, all three viruses are transmitted by whitefly.…”

Section: Introductionmentioning

confidence: 99%

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Identification of potential sources of mungbean yellow mosaic India virus resistance in black gram (Vigna mungo) and expression of antioxidants and R‐genes modulating resistance response in cultivated and its two wild relatives

Tripathi¹,

Singh²,

Kumar³

et al. 2023

Plant Breeding

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Black gram is one of the most important short duration grain legume, which contributes significantly towards nutritional security and environmental sustainability. The virus specific primers confirms the presence of mungbean yellow mosaic India virus (MYMIV) in representative samples. A total of 27 cultivated and two wild species were found as highly resistant (HR) to MYMIV and validated through molecular markers. The start codon target (SCoT) markers analysis revealed that the SCoT loci, namely, SCoT‐4 (2200 bp), SCot‐9 (1150/ 1200 bp), SCoT‐15 (1150/1100 bp), SCoT‐16 (700 bp), SCoT‐24 (2500 bp), SCoT‐25 (700 bp), SCoT‐33 (900/1000 bp), and SCoT‐34 (600 bp), were found unique, able to distinguish HR and highly susceptible (HS) genotypes. Biochemical characterization and gene expression profiling revealed the higher expression of antioxidants and R‐genes just after pathogen inoculation indicated the activation of defence mechanism in both cultivated and its wild relatives, which modulates the resistant responses in cultivated and wild accessions. These information will be really helpful in accelerating resistance breeding in black gram.

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Recent Advances in Omics Approaches for Mungbean Improvement

Singh¹,

Pratap

Kumar³

et al. 2022

Technologies in Plant Biotechnology and Breeding of Field Crops

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Microsatellite-based association mapping for agronomic traits in mungbean (Vigna radiata L. Wilczek)

Cited by 10 publications

References 33 publications

Genome wide association analysis for grain micronutrients and anti-nutritional traits in mungbean [Vigna radiata (L.) R. Wilczek] using SNP markers

Genome wide association analysis for grain micronutrients and anti-nutritional traits in mungbean [Vigna radiata (L.) R. Wilczek] using SNP markers

Identification of potential sources of mungbean yellow mosaic India virus resistance in black gram (Vigna mungo) and expression of antioxidants and R‐genes modulating resistance response in cultivated and its two wild relatives

Recent Advances in Omics Approaches for Mungbean Improvement

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