Microsatellite-based genetic diversity in selected exotic and indigenous maize (<i>Zea mays</i>L.) inbred lines differing in total kernel carotenoids

J. Plant Biochem. Biotechnol.

et al. 2015

Self Cite

Vitamin A deficiency caused by insufficient intake of β-carotene has emerged as one of the most important problems afflicting people worldwide. Traditional yellow maize is predominated by lutein and zeaxanthin; but contains low β-carotene par below the daily requirement for humans. A natural variant of crtRB1 allele increases β-carotene in kernel by blocking its conversion to zeaxanthin. In the present study, genetic diversity analyses were carried out among 24 diverse maize inbreds possessing rare allele of crtRB1. The mean β-carotene among the inbreds was 9.3 μg/g, with 14 inbreds having 9.1-18.8 μg/g. Among the inbreds of exotic origin, HP704-23, HP704-22, HP467-15, HP465-41 and HP467-20 were promising with high β-carotene. In case of inbreds developed in India, VQL-2-PV, VQL1-PV, V345-PV, V335-PV, HKI161-PV, MGU-PV-2, HKI1105-PV, HKI323-PV and MGU-PV-3 were identified to possess high kernel β-carotene. These inbreds with rare allele of crtRB1 are unique germplasm and therefore holds immense promise in the biofortification programme worldwide. Molecular profiling of these inbreds using 65 SSRs distributed throughout the genome generated 268 alleles with a mean of 4.12 alleles per locus. The polymorphism information content varied from 0.21 to 0.82 with a mean of 0.58. The study detected 12 each of unique and rare alleles. Genetic dissimilarity ranged from 0.40 to 0.94 with an average of 0.79. Cluster analyses grouped 24 genotypes into four major clusters, and principal coordinate analysis depicted the diverse nature of the genotypes consistent with their pedigree. The study identified potential hybrid combinations for higher β-carotene that can be directly utilized in the biofortification programme.

Section: Genetic Relationships Among Exotic and Indigenous Inbredsmentioning

confidence: 74%

Section: Genetic Relationships Among Exotic and Indigenous Inbredsmentioning

confidence: 97%

Section: Genetic Relationships Among Exotic and Indigenous Inbredsmentioning

confidence: 97%

See 1 more Smart Citation

Microsatellite marker-based genetic diversity analyses of novel maize inbreds possessing rare allele of β-carotene hydroxylase (crtRB1) for their utilization in β-carotene enrichment

Choudhary

J. Plant Biochem. Biotechnol.

et al. 2015

Self Cite

“…Also a total of 12 (5.63%) rare alleles were also identified, of which bnlg1113, bnlg1162, bnlg1621, phi077, bnlg1740, bnlg1191, umc1296, bnlg2191, umc1335 and phi314704 produced one rare allele each, and dupssr34 generated two rare alleles. Occurrence of unique-and rarealleles depends upon the number of genotypes analysed and type of SSR markers used in the study, and earlier studies have reported the occurrence of unique and rare alleles in maize inbreds differing for various quality traits (Sivaranjani et al 2014;Choudhary et al 2015;Muthusamy et al 2015).…”

Section: Assessment Of Genetic Variabilitymentioning

confidence: 99%

Microsatellite-based genetic diversity analyses of sugary1-, shrunken2- and double mutant- sweet corn inbreds for their utilization in breeding programme

Mehta

Physiol Mol Biol Plants

et al. 2017

Self Cite

Sweet corn has recently experienced sharp rise in demand worldwide. Recessive sugary1 (su1) and shrunken2 (sh2) that enhances kernel sweetness have been abundantly used in sweet corn breeding. Analyses of genetic diversity among sweet corn inbreds assume great significance for their effective utilization in hybrid breeding. A set of 48 diverse sweet corn genotypes encompassing su1su1, sh2sh2 and su1su1/sh2sh2 types were analyzed using 56 microsatellite markers. A total of 213 alleles with mean of 3.8 alleles per locus were generated. Two unique-and 12 rare-alleles were identified. The average PIC and genetic dissimilarity was 0.50 and 0.73, respectively. Cluster analysis grouped the inbreds into three major clusters, with each of the su1su1-, sh2sh2-and su1su1/sh2sh2-types were broadly clustered together. Principal coordinate analyses also depicted the diverse origin of the genotypes. The study identified inbreds for synthesis of pools and pedigree populations to develop novel inbreds. The study led to the identification of prospective heterotic combinations in various genetic backgrounds (sh2sh2 9 sh2sh2, su1su1 9 su1-su1, su1su1/sh2sh2 9 su1su1/sh2sh2, sh2sh2 9 su1su1/ sh2sh2 and su1su1 9 su1su1/sh2sh2).

“…Intensive efforts are currently being undertaken to develop maize inbreds enriched with carotenoids especially provitamin A Gupta et al 2013;Frano et al 2014). Though some reports are available on variability of carotenoids in maize germplasm especially those adapted to Indian conditions, however, they are based on variability of total carotenoids estimated through colorimetric methods (Mishra and Singh 2010;Tiwari et al 2012;Sivaranjani et al 2013Sivaranjani et al , 2014. Information regarding comprehensive analyses on components of carotenoids using precision-based HPLC methods, and their relationships among exotic and indigenous inbreds is lacking for designing suitable breeding strategy.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability and inter-relationship of kernel carotenoids among indigenous and exotic maize (Zea maysL.) inbreds

Cereal Research Communications

Thirunavukkarasu

et al. 2015

Carotenoids play vital role in growth and development of human beings. Yellow maize kernel contains carotenoids that possess provitamin A and antioxidant activity. Multilocation based analyses of 105 maize inbreds of indigenous and exotic origin revealed wide genetic variation for lutein (0.2-11.3 µg/g), zeaxanthin (0.2-20.0 µg/g) and β-carotene (0.0-15.0 µg/g). For β-cryptoxanthin, low variation (0.1-3.3 µg/g) was observed. Carotenoids were quite stable over environments that played minor role in causing variation. The heritability (>90%) and genetic advance (>75%) were high for all the carotenoid components. Zeaxanthin showed positive correlation with lutein and β-cryptoxanthin, while β-carotene, the major provitamin A carotenoid, did not show correlation with other carotenoids. Kernel colour was positively correlated with lutein (0.25), zeaxanthin (0.47) and β-cryptoxanthin (0.44), but not with β-carotene (0.04). This suggested that visual selection based on kernel colour will be misleading in selecting provitamin A-rich genotypes. Inbreds with provitamin A and non-provitamin A carotenoids identified in the present study will help in development of biofortified maize hybrids.