Microsatellite‐based parentage control in cattle

Abstract

As a new approach to parentage control we developed two multiplex coamplification polymerase chain reaction (PCR) systems containing a total of six different short tandem repeat (STR) loci; the microsatellite polymorphisms were visualized by automated fluorescence detection on the Applied Biosystems 373 DNA Sequencer with 672 Genescan Analysis software. Allele frequency data were determined from 238 animals. Thirty-five bovine parentage control cases not solvable by conventional blood typing could be solved.

Population studies of 16 bovine STR loci for forensic purposes

“…A second step in this process is the reporting of large-scale population data, similar to the standard operating procedure in humans and recently described in other nonhuman species relevant in forensic casework, such as cats [7,8] and dogs [9][10][11]. Measures including power of exclusion (PE) and the observed heterozygosity (HO) have been used in bovine parentage verification to assess the quality and power of routine analysis [12][13][14]. In this study, based on a data set of 4,162 animals representing 20 breeds, the efficacy of a set of 16 bovine STRs (BM1818, BM1824, BM2113, CSRM60, CSSM66, ETH3, ETH10, ETH225, HAUT27, ILSTS006, INRA023, SPS115, TGLA53, TGLA122, TGLA126, and TGLA227), all recommended by the Food and Agriculture Organization of the UN (www.…”

Genetic Characterization of Albanian Sheep Breeds by Microsatellite Markers

“…Microsatellite markers are currently the markers of choice for molecular genetic studies such as reconstruction of phylogenetic and relationships among populations (Bowcock et al, 1994;Forbes et al, 1995;MacHugh et al, 1997), determination of paternity and kinship analyses (Glowatzki-Mullis et al, 1995;Heyen et al, 1997;Luikart et al, 1999;Schlotterer, 2004) forensic studies (Edwards et al, 1992), linkage analysis (Francisco et al, 1996;Kappes et al, 1997;Mellersh et al, 1997), population structures (Arora and Bhatia, 2004;Bruford and Wayne, 1993).…”

Genetic Characterization and Polymorphisms for Parentage Testing of the Jeju Horse using 20 Microsatellite Loci

“…Microsatellite markers have also proven useful in assessing the genetic diversity of populations in different species [23]. In cattle [10], pigs [17], horses [5,19] and canines [4], pedigree control is performed on a routine basis in most countries relying on DNA typing that has been standardized through regular comparison tests under the auspices of the International Society for Animal Genetics (ISAG) [5].…”