Microsatellite-centromere distances and microsatellite diversity in different ploidy classes of Chinese shrimp (Fenneropenaeus Chinensis)

Abstract

This is the first report of microsatellite-centromere mapping in this commercial species Fenneropenaeus Chinensis, and will be important for providing fixed points in the linkage groups of genetic maps. Triploid Chinese shrimp was induced by heat shock. The fertilized eggs were treated either by retention of the first polar body or the second polar body to produce Meiosis I (MI) or Meiosis II (MII) triploid. The triploidy status in each Chinese shrimp could be confirmed by nine polymorphic microsatellite loci, in which the parents with different alleles and the female parents were each heterozygous. The nine loci were mapped in relation to their centromeres in three MII triploid families, which were induced by retention of the second polar bodies after fertilization with sperm. Microsatellite-centromere (M-C) distances ranged from 9.6 cM to 37 cM under the assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will represent a contribution towards assembly of genetic maps in F. chinensis. Twelve polymorphic microsatellites were used to assess the heterozygosity and allelic diversity in different ploidy classes. As expected, triploids were significantly more polymorphic than diploids. The diploids had an average heterozygosity and allelic diversity value of 0.86, whereas the triploids heterozygosity averaged 0.93 and had allelic diversity value of 1.29. However, MI triploids were not significantly more polymorphic than MII in the microsatellite loci.

Microsatellite-centromere mapping in Japanese scallop (Patinopecten yessoensis) through half-tetrad analysis in gynogenetic diploid families

“…Deviation from Mendelian segregation ratio has been observed in previous efforts of constructing molecular marker linkage maps. For microsatellite markers, distorted segregation was observed at 37.2% of loci in Pacific oyster (McGoldrick et al, 2000), 23.8% in sea cucumber (Li et al, 2009), and 11.8% in Chinese shrimp (Wang et al, 2008). The level of segregation distortion varies greatly among species, and likely reflects unique characteristics of different genomes.…”

Genetic Diversity and Differentiation of Fenneropenaeus Penicillatus Populations by Allozyme Analyses

“…However, unlike other major commercially important marine shrimps, such as Penaeus monodon [4], Marsupenaeus japonicus [5] and Fenneropenaeus chinensis [6], various techniques, including allozymes, random amplified polymorphic DNA(RAPDs), mitochondrial DNA(mtDNA) and genotypic repeat sequences, have been utilized for their genetic studies. Up until now, little research has been done on genetic aspects of F. penicillatus, especially concerning its genetic diversity and differentiation.…”

Choice of microsatellite markers for identifying homozygosity of mitotic gynogenetic diploids in Japanese flounder Paralichthys olivaceus

“…rerio (Kauffman et al ., ), rainbow trout Oncorhynchus mykiss (Walbaum 1792) (Sakamoto et al ., ), carp Cyprinus carpio L. 1758 (Aliah & Taniguchi, ), M . anguillicaudatus (Morishima et al ., ), Pacific abalone Haliotis discus hannai (Li & Kijima, ), Japanese eel Anguilla japonica Temminck & Schlegel 1846 (Nomura et al ., ), Japanese abalone Haliotis kamtschatkana (Sekino et al ., ), Pacific oyster Crassostrea gigas (Li & Kijima, ), barfin flounder Verasper moseri Jordan & Gilbert 1898 (Lahrech et al ., ), yellow croaker Pseudosciaena crocea (Richardson 1846) (Li et al ., ), turbot Scophthalmus maximus (L. 1758) (Martínez et al ., ), Chinese shrimp Fenneropenaeus chinensis (Wang et al ., ) and half‐smooth tongue sole Cynoglossus semilaevis Günther 1873 (Ji et al ., ).…”