Microsatellite characterization of Andean races of common bean (Phaseolus vulgaris L.)

Blair, Matthew W.; Díaz, Juan Manuel; Hidalgo, Rigoberto; Díaz, Lucy M.; Duque, Myriam C.

doi:10.1007/s00122-007-0644-8

Cited by 90 publications

(138 citation statements)

References 39 publications

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“…Andean diversity was found to be relatively high but diYcult to subdivide with the Andean genepool control genotypes, Calima and G19833, representing Nueva Granada and Peru races, respectively (Blair et al 2007) clustered together in both principal coordinate and population structure analyses. The closer placement of the two Andean control genotypes and concomitant overlap with other accessions from Ethiopia and Kenya might indicate the representation of the East African Andean genotypes, especially those from Kenya as part of a race Nueva Granada/ race Peru complex.…”

Section: Discussionmentioning

confidence: 97%

“…cgiar.org/urg/beans.htm) with 99 genotypes from Ethiopia and 89 genotypes from Kenya and the majority being landraces and only a few being commercial cultivars (supplementary information). The control genotypes were selected on the basis of their use in previous studies with microsatellite markers (Blair et al 2006;Díaz and Blair 2006;Blair et al 2007). These were: 'Calima' (G4494) a variety from Colombia, and 'Chauca Chuga' (G19833), a landrace from Peru as the Andean control genotypes; as well as DOR364 (or 'Dorado'), a variety from CIAT/El Salvador, and ICA Pijao, a variety from Colombia as Mesoamerican controls.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The fragment analysis data from ABI3730 system were analyzed and allele sizes scored with GENEMAPPER version 3.7 software (Applied Biosystems). The observed allele size was then adjusted for the discrete allele size using AlleloBin software (http:// test1.icrisat.org/gt-bt/download_allelobin.htm) and allele sizes for the control genotypes (Calima, G19833, DOR364 and ICA Pijao) were conWrmed to be of the same sizes as in Blair et al (2006Blair et al ( , 2007 and Díaz and Blair (2006).…”

Section: Microsatellite Marker Evaluationmentioning

confidence: 99%

“…Gene Xow within and between genepools and races via spontaneous out-crossing in farmer's Weld or crossing programs in formal breeding could result in intermediate phenotypes that do not correspond well to any of the single race or genepool divisions (Beebe et al 2001;Islam et al 2004;Díaz and Blair 2006;Blair et al 2007). Understanding the pattern of population-genetic structure and diversity of bean landraces and cultivars (hereafter accessions) and their relationships with the Andean and Mesoamerican genepools can therefore provide information on gene Xow and be of great importance for future common bean breeding in the region.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands

Asfaw¹,

2009

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The East African highlands are a region of important common bean production and high varietal diversity for the crop. The objective of this study was to uncover the diversity and population structure of 192 landraces from Ethiopia and Kenya together with four genepool control genotypes using morphological phenotyping and microsatellite marker genotyping. The germplasm represented diVerent common bean production ecologies and seed types common in these countries. The landraces showed considerable diversity that corresponded well to the two recognized genepools (Andean and Mesoamerican) with little introgression between these groups. Mesoamerican genotypes were predominant in Ethiopia while Andean genotypes were predominant in Kenya. Within each country, landraces from diVerent collection sites were clustered together indicating potential gene Xow between regions within Kenya or within Ethiopia. Across countries, landraces from the same country of origin tended to cluster together indicating distinct germplasm at the national level and limited gene Xow between the two countries highlighting divided social networks within the regions and a weak trans-national bean seed exchange especially for landrace varieties. One exception to this may be the case of small red-seeded beans where informal cross-border grain trade occurs. We also observed that genetic divergence was slightly higher for the Ethiopian landraces compared to Kenyan landraces and that Mesoamerican genotypes were more diverse than the Andean genotypes. Common beans in eastern Africa are often cultivated in marginal, riskprone farming systems and the observed landrace diversity should provide valuable alleles for adaptation to stressful environments in future breeding programs in the region.

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Section: Discussionmentioning

confidence: 97%

Section: Plant Materialsmentioning

confidence: 99%

Section: Microsatellite Marker Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces from the East African highlands

Asfaw¹,

2009

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“…La diversidad genética entre los grupos identifi cados en el dendrograma basada en distancias genéticas de Nei (Nei 1978) se calculó utilizando el programa POPGENE versión 1.32 (Yeh et al 1997). La matriz utilizada para este programa se construyó representando cada banda o alelo amplifi cado como un genotipo diferente dada la dominancia de los AFLPs (Wagara et al 2004 (Blair et al 2007). El fl ujo genético es igual al número de migrantes entre poblaciones por generación calculada en base a la fór-mula Nm=0,5(1-G ST )/G ST (Blair et al 2007).…”

Section: Materiales Y Métodosunclassified