Microsatellite development via next-generation sequencing in <i>Acacia stenophylla</i> (Fabaceae) and <i>Duma florulenta</i> (Polygonaceae): two ecologically important plant species of Australian dryland floodplains

Murray, Bruce F; Reid, Michael; Wu, Shu‐Biao

doi:10.7287/peerj.preprints.27027

Cited by 2 publications

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“…All samples were genotyped using 13 microsatellite markers reported for A. stenophylla by Murray, Reid, and Wu () (Genbank accessions –). To reduce the costs by multiplexing, PCR products were labelled using M13 universal primers as outlined in Sheulke ().…”

Section: Methodsmentioning

confidence: 99%

“…All samples were genotyped using 13 microsatellite markers reported for A. stenophylla by Murray, Reid, and Wu (2018) A touchdown PCR program was used, which consisted of an initial denaturation step of 5 min at 94°C followed by three cycles of denaturation for 30 s at 94°C, annealing for 45 s at 60°C and elongation for 45 s at 72°C. This step was repeated for three cycles with annealing temperatures of 57 and 54°C and then for 30 cycles at an annealing temperature of 52°C.…”

Section: Genotypingmentioning

confidence: 99%

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Gene flow and genetic structure in Acacia stenophylla (Fabaceae): Effects of hydrological connectivity

Murray

Reid

Capon

et al. 2019

Journal of Biogeography

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Aim Riparian ecosystems are regarded as vulnerable to the effects of climate change. Because of their reliance on passive dispersal to migrate from areas where conditions have become unfavourable, plants are particularly susceptible. On dryland river floodplains, the species diversity of herbaceous annuals is often high while that of structurally dominant woody perennials is low. We examined gene flow genetic structure and dispersal in Acacia stenophylla, a small perennial tree widely distributed throughout river systems of inland Australia. The role of the river corridor in shaping patterns of gene flow and genetic structure is also investigated. Location Murray‐Darling Basin, south eastern Australia Methods A total of 127 individuals, from 12 subpopulations located on seven rivers were genotyped at 13 microsatellite loci. Several population and landscape genetic tools were applied to the microsatellite data to evaluate spatial patterns of gene flow and genetic structure and make inferences regarding possible modes of dispersal. Results High gene flow and weak genetic structure was identified for the 12 subpopulations of A. stenophylla sampled, a surprising result given large distances between subpopulations. Pairwise genetic distance between subpopulations was low to moderate and could largely be explained (R2 = 0.68) by two variables: distance along the river and the proportion of no flow days. structure analysis revealed two genetic clusters. Subpopulations located on the Darling and Lower Balonne rivers were dominated by cluster one while subpopulations from the Warrego and Paroo rivers showed largely mixed ancestry with individuals descending from both clusters one and two. Main Conclusions These results indicate that the river corridor facilitates extensive gene flow between subpopulations of A. stenophylla in this system. Hydrochory appears to be the dominant process; however, upstream movements of propagules most probably via animal movement are sufficient to negate effects expected under unidirectional dispersal.

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Section: Methodsmentioning

confidence: 99%

Section: Genotypingmentioning

confidence: 99%

Gene flow and genetic structure in Acacia stenophylla (Fabaceae): Effects of hydrological connectivity

Murray

Reid

Capon

et al. 2019

Journal of Biogeography

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“…Samples were genotyped at 12 microsatellite loci reported for lignum by Murray, Reid, and Wu () (Genbank accession numbers –). To reduce the costs by multiplexing, PCR products were labeled using M13 universal primers as outlined in Sheulke ().…”

Section: Methodsmentioning

confidence: 99%

Genetic analysis suggests extensive gene flow within and between catchments in a common and ecologically significant dryland river shrub species; Duma florulenta (Polygonaceae)

Murray

Reid

Capon

et al. 2019

Ecology and Evolution

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Aim The conservation of plant species biodiversity has been identified as a crucial factor for the resilience of dryland ecosystems in the face of climate change and desertification. Duma florulenta (lignum) is a keystone species that facilitates biodiversity in the floodplains and wetlands of Australia's dryland river systems. This paper explores spatial genetic structure of lignum and investigates factors influencing dispersal and gene flow within and among river catchments of the northern Murray–Darling Basin. Location Northern Murray–Darling Basin, eastern Australia. Methods A total of 122 individual plants from subpopulations located on rivers in four adjacent catchments were genotyped using 10 microsatellite markers. Microsatellite data were then analyzed using population genetic techniques to evaluate levels of gene flow and genetic structure and identify factors influencing dispersal. Results Results suggest high levels of gene flow between lignum subpopulations of the northern Murray–Darling Basin. AMOVA revealed small but significant differences between subpopulations, and STRUCTURE analysis did not detect meaningful structure when sampling information was not provided. However, when sampling information was supplied using the LOCPRIOR model, three genetic clusters were identified. All Lower Balonne subpopulations were assigned to cluster 1 while a number of the other subpopulations showed mixed ancestry. Weak relationships were identified between pairwise genetic distance and geographic as well as river distance, although the R 2 value of the former was only half that of the latter. Main conclusions Patterns of genetic variation suggest frequent long‐distance overland gene flow largely as a result of the movement of seeds via floodwater. Therefore, maintenance of natural variability in flow regime is key both to maintain conditions favorable to recruitment and to promote dispersal and gene flow across the landscape. However, given future climate change projections persistence may be more reliant on the species ability to endure long periods of drought between flood events.

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Microsatellite development via next-generation sequencing in Acacia stenophylla (Fabaceae) and Duma florulenta (Polygonaceae): two ecologically important plant species of Australian dryland floodplains

Cited by 2 publications

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Gene flow and genetic structure in Acacia stenophylla (Fabaceae): Effects of hydrological connectivity

Gene flow and genetic structure in Acacia stenophylla (Fabaceae): Effects of hydrological connectivity

Genetic analysis suggests extensive gene flow within and between catchments in a common and ecologically significant dryland river shrub species; Duma florulenta (Polygonaceae)

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