Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in the Popular Dog Breeds in Poland

Radko, Anna; Podbielska, Angelika

doi:10.3390/genes12040485

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…The results show that the breed has a high level of genetic diversity. The number of alleles that have the greatest genetic influence on heterozygosity, known as Ne, averaged 4.869 in the general population, which is higher than the Ne reported for other native dog breeds: 3.0-3.5 for the Italian Greyhound [28], 3.3 for the German Shepherd, 3.5 for the Maltese, 3.2 for the Biewer Yorkshire Terrier, 3.5 for the Yorkshire Terrier [29]. All markers were highly informative (PIC values greater than 0.5) [30], suggesting the efficacy of these markers for the assessment of genetic variation among the Tazy dogs.…”

Section: Discussionmentioning

confidence: 72%

Kazakh national dog breed Tazy: What do we know?

et al. 2023

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The Tazy or Kazakh National sighthound has been officially recognized as the national heritage of Kazakhstan. Comprehensive genetic studies of genetic diversity and population structure that could be used for selection and conservation of this unique dog breed have not been conducted so far. The aim of this study was to determine the genetic structure of the Tazy using microsatellite and SNP markers and to place the breed in the context of the world sighthound breeds. Our results showed that all 19 microsatellite loci examined were polymorphic. The observed number of alleles in the Tazy population varied from 6 (INU030 locus) to 12 (AHT137, REN169D01, AHTh260, AHT121, and FH2054 loci) with a mean of 9.778 alleles per locus. The mean number of effective alleles was 4.869 and ranged from 3.349 f to 4.841. All markers were highly informative (PIC values greater than 0.5) and ranged from 0.543 (REN247M23 locus) to 0.865 (AHT121 locus). The observed and expected heterozygosities in a total population were 0.748 and 0.769 and ranged from 0.746 to 0.750 and 0.656 to 0.769, respectively. Overall, the results confirmed that the Tazy breed has a high level of genetic diversity, no significant inbreeding, and a specific genetic structure. Three gene pools underlie the genetic diversity of the Tazy breed. SNP analysis using the CanineHD SNP array, which contains more than 170,000 SNP markers, showed that the Tazy breed is distinct from other sighthound breeds and genetically related to ancient eastern sighthound breeds sharing the same branch with the Afghan Hound and the Saluki. The results, together with archeological findings, confirm the ancient origin of the breed. The findings can be used for the conservation and international registration of the Tazy dog breed.

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Section: Discussionmentioning

confidence: 72%

Kazakh national dog breed Tazy: What do we know?

et al. 2023

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“…Although pedigree data are important for determining genetic diversity, they are limited by the start of recording and missing or incorrect recordings [ 20 ]. The availability of genomic data has facilitated landscape genomics and conservation studies to accurately estimate genetic diversity and inbreeding [ 8 , 21 , 22 ]. Genomic data enable the accurate assessment of a population structure based on the actual versus expected genetic diversity [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Pedigree-Based Genetic Diversity in the South African Boerboel Dog Breed

Mabunda,

Nephawe,

Mtileni

et al. 2024

Animals

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The Boerboel dog breed (BBD) is indigenous to South Africa (SA) and plays an important role in safeguarding homes and farms. The Department of Agriculture, Land Reform, and Rural Development (DALRRD) classifies the BBD as a protected species, and it is valued for its intelligence, boldness, and strength, as well as for continually ensuring the safety of its owners. The aim of this study was to investigate genetic diversity within the BBD population using pedigree information. The original BBD data, which contained 87,808 records, were obtained from the Integrated Registration and Genetic Information System (INTERGIS). After editing, the pedigree data included 87,755 records of animals born between 1971 and 2019. Pedigree analyses were performed using PEDIG (Fortran 77 software) to determine the completeness, inbreeding coefficients, and genetic diversity as defined by the genetic contributions of the most important ancestors of the current animals. This study identified 91.2% inbred animals in the BBD population, with an average and maximum inbreeding of 7.5% and 50% of inbred animals, respectively. The estimated inbreeding rate per year was 0.20% with an effective population size of 83.1. The most influential ancestors explained 82.63% and 80.92% of the total genetic variation for males and females in the studied populations, respectively. Only 10 important ancestors explained more than 50% of the entire population’s genetic diversity. The numbers of founders (f) were 348 and 356, and the effective numbers of founders (fe) were 57.4 and 60.1, respectively, for males and females. The numbers of founders were higher than the effective numbers of founders, implying a loss of genetic diversity due to unequal founder contributions. The BBD population was not critically endangered based on the inbreeding rates and effective population size; however, the population experienced a significant loss of genetic variability, unequal genetic contributions by founders, and a genetic bottleneck. Future breeding strategies could benefit from using equal proportions of parent stock and including new genetically distant breeds.

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“…Therefore, their study indicated that the tested Tatra shepherd dog population was highly polymorphic. In another study [ 29 ], eight breeds of dog were surveyed by 21 microsatellite markers. PIC values over 0.5 were measured for 15 markers.…”

Section: Discussionmentioning

confidence: 99%

Development of novel microsatellite markers to analyze the genetic structure of dog populations in Taiwan

et al. 2022

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Objective: Alongside the rise of animal-protection awareness in Taiwan, the public has been paying more attention to dog genetic deficiencies due to inbreeding in the pet market. The goal of this study was to isolate novel microsatellite markers for monitoring the genetic structure of domestic dog populations in Taiwan.Methods: A total of 113 DNA samples from three dog breeds -beagles (BEs), bichons (BIs), and schnauzers (SCs) -were used in subsequent polymorphic tests applying the 14 novel microsatellite markers that were isolated in this study. Results:The results showed that the high level of genetic diversity observed in these novel microsatellite markers provided strong discriminatory power. The estimated probability of identity (P(ID)) and the probability of identity among sibs (P(ID)sib) for the 14 novel microsatellite markers were 1.7×10 -12 and 1.6×10 -5 , respectively. Furthermore, the power of exclusion (PE) for the 14 novel microsatellite markers was 99.98%. The neighbor-joining (NJ) trees constructed among the three breeds indicated that the 14 sets of novel microsatellite markers were sufficient to correctly cluster the BEs, BIs, and SCs. The principal coordinate analysis (PCoA) plot showed that the dogs could be accurately separated by these 14 loci baled on different breeds; moreover, the Beagles from different sources were also distinguished. The first, the second, and the third principal coordinates could be used to explain 44.15, 26.35 and 19.97% of the genetic variation. Conclusion:The results of this study could enable powerful monitoring of the genetic structure of domestic dog populations in Taiwan.

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Microsatellite DNA Analysis of Genetic Diversity and Parentage Testing in the Popular Dog Breeds in Poland

Cited by 13 publications

References 32 publications

Kazakh national dog breed Tazy: What do we know?

Kazakh national dog breed Tazy: What do we know?

Pedigree-Based Genetic Diversity in the South African Boerboel Dog Breed

Development of novel microsatellite markers to analyze the genetic structure of dog populations in Taiwan

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