Microsatellite DNA Loci for Population Studies in Brazilian Chicken Ecotypes

Clementino, C. S.; Barbosa, Firmino José Vieira; Carvalho, Adriana Márcia Ferreira de; Costa-Filho, R. S. R; Silva, G. R.; Campelo, E. G.; Britto, Fábio Barros; Diniz, F. M.

doi:10.3923/ijps.2010.1100.1106

Cited by 12 publications

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“…In this study, all markers had PIC higher than 0.5, showing high level of polymorphism in all the analyzed microsatellite loci. The PIC values were similar as reported by CLEMENTINO et al (2010) in one study with Brazilian free-range chickens, which had used six of the same markers used in this research. Likewise, FONTEQUE et al (2014) found the PIC values similar of these, using, among others, the same microsatellites loci of this research in Brazilian (blue-egg Caipira) chickens.…”

Section: 794 -------------------------------------------------------supporting

confidence: 69%

“…CLEMENTINO et al (2010) andFONTEQUE et al (2014) found an average number of 9.6 and 12.4 alleles per locus, respectively, in studies with microsatellites in free range chickens from Brazil. Lower values than the results found in this study were also described by AL-QAMASHOUI et al (2014) - 8.4 (VANHALA et al, 1998;KYSER et al, 2000;ZHANG et al, 2002;HILLEL et al, 2003;TADANO et al, 2007;BERTHOULY et al, 2008;ROSÁRIO et al, 2009;CHATTERJEE et al, 2010;TADANO et al, 2012;WILKINSON et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…LIMA-ROSA et al (2004) investigated the polymorphism of two genes B-F (genes of the immune system of the chicken) from Brazilian (blue-egg Caipira) chickens samples. CLEMENTINO et al (2010) examined the variability of microsatellite markers in free range chickens from northeastern Brazil. FONTEQUE et al (2014) identified the genetic variation of microsatellite loci from a population of Brazilian (blue-egg Caipira) chickens.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

et al. 2015

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Section: 794 -------------------------------------------------------supporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

et al. 2015

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“…This significant variation is evident when comparing these data with those found for the same loci in other works. Clementino (2010) and Clementino et al (2010) examined 92 samples from four populations of Caipira chickens (called for them of Brazilian chicken ecotypes) from northeast region of Brazil with ten loci of microsatellites, and encountered 14-16 alleles to locus LEI0192, 8-12 alleles to LEI0221, 9-13 alleles to LEI0217, and 9-10 alleles to LEI0234, in populations with smaller and larger number of alleles respectively. Nasiri et al (2007) reported one locus (MCW0216) monomorphic and other (ADL0278) with only three alleles in a population of Iranian native chicken.…”

Section: Resultsmentioning

confidence: 99%

Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

et al. 2014

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The purpose of this study was to investigate the genetic polymorphism of fifteen microsatellites loci in Brazilian (blue-egg Caipira) chickens. Samples were collected from 100 blue eggs of Caipira chickens from rural properties in the city of Dois Lajeados, RS. After DNA extraction, the fragments related to molecular markers LEI0248, LEI0221, LEI0214, LEI0192, LEI0217, LEI0254, LEI0194, LEI0212, MCW0371, ADL0278, LEI0234, MCW0183, MCW0216, MCW0330 and MCW0081 were obtained by polymerase chain reaction (PCR). The statistical analysis were carried out with the softwares ARLEQUIN 3.5 version and CER-VUS 3.0.3 version. The allelic and genotypic frequencies, deviations from Hardy-Weinberg equilibrium, estimates of observed (HO) and expected (HE) heterozygosity and polymorphic information content (PIC) were obtained for each marker locus. A total of 186 alleles from 15 loci were obtained, with sizes ranging of 83 to 490 base pairs. The medium number of alleles was 12.4, the HE was 0.76±0.14 and HO was 0.49±0.21 and PIC was 0.706. The first conclusion is that the microsatellites used are polymorphic and can be used to genetic studies in chickens. The second is that the "Caipira" chicken (blue eggs) population investigated has a great genic variability, which makes than an important source of genetic resources for future animal breeding programs. Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens nhas caipiras de ovos azuis analisada apresenta grande variabilidade gênica, o que as torna uma importante fonte de recursos genéticos, e que poderão, assim, serem utilizadas em futuros programas de melhoramento genético animal.

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“…Dos marcadores disponíveis, os microssatélites, sequências de DNA curtas repetidas em tandem, são os mais usados devido ao grande volume de informações fornecidas (Clementino et al, 2010). No entanto, o desenvolvimento desses marcadores é de alto custo e a técnica requer o emprego de métodos mais avançados da biologia (Francisco et al, 2013).…”

Section: Introductionunclassified

Aspectos bioecológicos e genético-comportamentais envolvidos na conservação da abelha Jandaíra, Melipona subnitida Ducke (Apidae, Meliponini), e o uso de ferramentas moleculares nos estudos de diversidade

et al. 2014

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A abelha sem ferrão Melipona subnitida atualmente está presente em quase toda a região nordeste, em função da boa adaptabilidade ao semiárido nordestino e do potencial econômico-ecológico proporcionado pela produção de mel e pela polinização de cultivos em condições de confinamento. Apesar disso, é uma espécie ameaçada devido a processos de degradação ambiental, dentre os quais estão o desmatamento, o uso indiscriminado de agrotóxicos e o extrativismo. Tais interferências tendem a isolar as populações de Jandaíra, provocando uma queda na variabilidade genética e, consequentemente, uma redução na capacidade adaptativa da espécie. Porém, técnicas de biologia molecular estão sendo implementadas, possibilitando que populações desse tipo sejam avaliadas quanto ao seu grau de variabilidade genética. Marcadores moleculares do tipo microssatélites de DNA vêm sendo bastante usados, porém, em função do alto custo exigido para seu desenvolvimento, diversos estudos vêm empregando microssatélites transferidos de táxons próximos com amplo sucesso em estudos voltados à caracterização e à diversidade genética. Dessa forma, a presente revisão objetivou avaliar os mais relevantes aspectos bioecológicos e genético-comportamentais envolvidos na conservação da abelha Jandaíra, a fim de auxiliar na avaliação do grau de diversidade genética da espécie, bem como da sua distribuição entre indivíduos e populações da abelha sem ferrão M. subnitida.

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Microsatellite DNA Loci for Population Studies in Brazilian Chicken Ecotypes

Cited by 12 publications

References 4 publications

Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

Genetic polymorphism of fifteen microsatellite loci in Brazilian (blue-egg Caipira) chickens

Aspectos bioecológicos e genético-comportamentais envolvidos na conservação da abelha Jandaíra, Melipona subnitida Ducke (Apidae, Meliponini), e o uso de ferramentas moleculares nos estudos de diversidade

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