Microsatellite DNA marker aided diversity analysis in frenchbean (<i>Phaseolus vulgaris</i>L.)

Nishimwe, Immaculee; M, Uma; Hegde, Niranjana; Nagaraja, T. E.; Somashekahar, Y.M.

doi:10.5958/0976-0571.2015.00003.x

Cited by 2 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, our results suggest that ISSR-PCR products generated with di-nucleotide motif primers can be useful markers for analyses of French bean germplasms. Similarity value based on Jaccard's coefficient clearly demonstrates that overall similarity between genotypes was low, reflecting strong genetic differentiation among the lines tested and our results were in accordance with previous investigators (Alghamdi et al 2011;Immaculee et al 2015, Jannat et al 2022.…”

Section: Polymorphism Of Genotypes Based On Issr Markerssupporting

confidence: 92%

Genetic Divergence in French Bean Accessions

Panda¹,

Paul²

2023

View full text Add to dashboard Cite

Background: Crop improvement has paramount importance to meet the global demand of food to feed the growing human population. Methods: A total of 23 French bean genotypes with diverse genetic background were taken to assess their phenotypic attributes and molecular characterization using inter simple sequence repeat (ISSR) markers. The data collected during two growing seasons over four locations on different quantitative characters were pooled and analyzed under randomized complete block design for deciphering the extent of genetic diversity. Similarity value, based on Jaccard’s coefficient and UPGMA (un-weighted pair group method with arithmetic mean) analysis was calculated. The dendrogram was constructed using Jaccard’s similarity matrix for above analysis. Result: The genotypes showed a wide variation in morphological characters like plant height, pods per plant, pod weight, pod length and pod yield per plant. The genotype HAFB-5 showed the maximum potential for pod yield per plant and long pods. Four ISSR primers used in this study amplified 27 reproducible bands with 18 (66.66%) polymorphic. The genotype Kashi Param and HAFB-4 were found diverse among the selected genotypes on the basis of molecular markers.

show abstract

Section: Polymorphism Of Genotypes Based On Issr Markerssupporting

confidence: 92%

Genetic Divergence in French Bean Accessions

Panda¹,

Paul²

2023

View full text Add to dashboard Cite

show abstract

“…Comparative studies in legumes involving SSR have been successfully used by few researchers either as individual marker or in combination in crops like Vigna unguiculata (Gillaspie et al, 1996) and Vigna sp. (Dikshit et al, 2007), Phaseolus vulgaris (Immaculee et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Molecular characterization of wilt resistant and susceptible genotypes of <italic>Cajanus cajan</italic> (L.)

Swami

Mahatma

Parekh

et al. 2015

View full text Add to dashboard Cite

Characterization of germplasm is important for breeding programme. Therefore, in present investigation 9 genotypes of pigeonpea [<italic>Cajanuscajan</italic> (L) Millsp] comprising three wilt resistant genotypes (Vaishali, ICPL 87119 and national check ICP 8863) and two susceptible genotypes (T<sub>15-15</sub> and national check ICP 2376) along with four other popular genotypes grown in Gujarat, India (BDN-2, GT-100, GT-102 and C-11) were characterized using RAPD, ISSR and SSR markers. Genetic diversity of 9 pigeonpea genotypes revealed an average polymorphism of 39.18% by RAPD, 47.67 % by ISSR and 57.35 % by SSR. On the basis of polymorphism (%), PIC(polymorphic information content) value, MI (Marker index) value primer OPI-12 was found most informative among 12 RAPD primers. While ISSR primer UBC-850 was found most informative among 8 ISSR primers showing 80% polymorphism, 0.51 PIC value and 40.80 MI value. SSR primers gave 2 to 5 amplicon on a 6 % polyacrylamide gel. The average PIC value was 0.36, ranging from 0 to 0.73. PIC also provides the estimate of discriminatory power of a locus in the case of SSR marker system by taking into account not only the number of alleles expressed, but also the relative frequency of those alleles. Our prime target of discrimination between wilt resistant and susceptible genotypes of pigeon pea was achieved by ISSR and SSR markers. However, SSR was more efficient in generation of polymorphism among the genotypes based on the information on the level of polymorphism and the PIC value.

show abstract

Microsatellite DNA marker aided diversity analysis in frenchbean (Phaseolus vulgarisL.)

Cited by 2 publications

References 17 publications

Genetic Divergence in French Bean Accessions

Genetic Divergence in French Bean Accessions

Molecular characterization of wilt resistant and susceptible genotypes of <italic>Cajanus cajan</italic> (L.)

Contact Info

Product

Resources

About