Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (<i>Dendroica kirtlandii</i>)

King, Tim L.; Eackles, Michael S.; Henderson, Anne P.; Bocetti, Carol I.; Currie, David J.; Wunderle, Joseph M.

doi:10.1111/j.1471-8286.2005.00998.x

Cited by 15 publications

(5 citation statements)

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“…Dried samples found application in populational genetics studies as a convenient instrument of sample collection and investigation (Sacks et al 2004 ; Silva et al 2009 ; Lall et al 2010 ; Kraus et al 2013 ; Guerrini et al 2014 ; Kashiwagi et al 2015 ; Nunziata et al 2016 ). Isolation and characterization of DNA microsatellite markers, their genotyping for population genetic studies was also described in other works (Sacks et al 2004 ; King et al 2005 ; Lucentini et al 2006 ; Carr and Appleyard 2008 ; Heim et al 2012 ; Nunziata et al 2016 ). Kashiwagi et al ( 2015 ) originally described an underwater collection of manta ray mucus by SCUBA divers for further application on FTA Elute cards for population genetic studies.…”

Section: Dbs Samples In Veterinary Medicine and Animal Biology: Diagn...mentioning

confidence: 87%

“…The authors concluded that preservation FTA cards were not adequate for providing unfragmented DNA for downstream genomic applications such as microsatellite and genomic sequencing especially for mammals which have non-nucleated blood cells. However, other researches successfully used FTA cards for genetic applications such as microsatellite genotyping (King et al 2005 ; Lucentini et al 2006 ; Carr and Appleyard 2008 ; Heim et al 2012 ; Nunziata et al 2016 ). For example, Lucentini et al ( 2006 ) described a nondestructive method for obtaining the high-quality DNA from fish body mucus and buccal cells, making it the best choice for populational genotyping.…”

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

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Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

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Dried Blood Spots (DBS) technology has become a valuable tool in medical studies, however, in veterinary and biological research DBS technology applications are still limited. Up-to-date no review has comprehensively integrated all the evidence existing across the fields, technologies and animal species. In this paper we summarize the current applications of DBS technology in the mentioned areas, and provide a scope of different types of dried sample carriers (cellulose and non-cellulose), sampling devices, applicable methods for analyte extraction and detection. Mammals, birds, insects and other species are represented as the study objects. Besides the blood, the review considers a variety of specimens, such as milk, saliva, tissue samples and others. The main applications of dried samples highlighted in the review include epidemiological surveys and monitoring for infections agents or specific antibodies for disease/vaccination control in households and wildlife. Besides the genetic investigations, the paper describes detection of environmental contaminants, pregnancy diagnosis and many other useful applications of animal dried samples. The paper also analyses dried sample stability and storage conditions for antibodies, viruses and other substances. Finally, recent developments and future research for DBS technology in veterinary medicine and biological sciences are discussed.

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Section: Dbs Samples In Veterinary Medicine and Animal Biology: Diagn...mentioning

confidence: 87%

Section: Carriers For Dried Samples and Sample Pretreatmentmentioning

confidence: 99%

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

2022

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“…; King et al . ). For each locus, GenBank accessions were assigned chromosome locations in the zebra finch genome assemblies Taeniopygia_guttata ‐3.2.4 (v 1.1), by performing a blat (Kent ) search using the ensembl webpage (http://www.ensembl.org/Multi/blastview).…”

Section: Methodsmentioning

confidence: 97%

Level of genetic differentiation affects relative performances of expressed sequence tag and genomic SSRs

Khimoun

Ollivier

Faivre

et al. 2017

Molecular Ecology Resources

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Microsatellites, also called simple sequence repeats (SSRs), are markers of choice to estimate relevant parameters for conservation genetics, such as migration rates, effective population size and kinship. Cross-amplification of SSRs is the simplest way to obtain sets of markers, and highly conserved SSRs have recently been developed from expressed sequence tags (EST) to improve SSR cross-species utility. As EST-SSRs are located in coding regions, the higher stability of their flanking regions reduces the frequency of null alleles and improves cross-species amplification. However, EST-SSRs have generally less allelic variability than genomic SSRs, potentially leading to differences in estimates of population genetic parameters such as genetic differentiation. To assess the potential of EST-SSRs in studies of within-species genetic diversity, we compared the relative performance of EST- and genomic SSRs following a multispecies approach on passerine birds. We tested whether patterns and levels of genetic diversity within and between populations assessed from EST- and from genomic SSRs are congruent, and we investigated how the relative efficiency of EST- and genomic SSRs is influenced by levels of differentiation. EST- and genomic SSRs ensured comparable inferences of population genetic structure in cases of strong genetic differentiation, and genomic SSRs performed slightly better than EST-SSRs when differentiation is moderate. However and interestingly, EST-SSRs had a higher power to detect weak genetic structure compared to genomic SSRs. Our study attests that EST-SSRs may be valuable molecular markers for conservation genetic studies in taxa such as birds, where the development of genomic SSRs is impeded by their low frequency.

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“…We used 17 microsatellite loci (Table 2 ), 12 of these loci were developed specifically for Kirtland’s warblers [ 44 ]. Five other loci Dpu16 [ 45 ], Lswu07 [ 46 ] and Vecr04, Vecr08, Vecr10 were developed for other species [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

Temporal patterns of genetic diversity in Kirtland’s warblers (Dendroica kirtlandii), the rarest songbird in North America

2012

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BackgroundKirtland’s warblers are the rarest songbird species in North America, rarity due in part to a reliance on early successional Jack Pine forests. Habitat loss due to fire suppression led to population declines to fewer than 200 males during the 1970s. Subsequent conservation management has allowed the species to recover to over 1700 males by 2010. In this study, we directly examine the impact that low population sizes have had on genetic variation in Kirtland’s warblers. We compare the molecular variation of samples collected in Oscoda County, Michigan across three time periods: 1903–1912, 1929–1955 and 2008–2009.ResultsIn a hierarchical rarified sample of 20 genes and one time period, allelic richness was highest in 1903–1912 sample (ar = 5.96), followed by the 1929–1955 sample (ar = 5.74), and was lowest in the 2008–2009 sample (ar = 5.54). Heterozygosity measures were not different between the 1929–1955 and 2008–2009 samples, but were lower in the 1903–1912 sample. Under some models, a genetic bottleneck signature was present in the 1929–1955 and 2008–2009 samples but not in the 1903–1912 sample.ConclusionsWe suggest that these temporal genetic patterns are the result of the declining Kirtland’s warbler population compressing into available habitat and a consequence of existing at low numbers for several decades.

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Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (Dendroica kirtlandii)

Cited by 15 publications

References 10 publications

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives

Level of genetic differentiation affects relative performances of expressed sequence tag and genomic SSRs

Temporal patterns of genetic diversity in Kirtland’s warblers (Dendroica kirtlandii), the rarest songbird in North America

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