Microsatellite DNA polymorphisms for colony management of longtailed macaques (Macaca fascicularis) population on the Tinjil Island

Farajallah, Dyah Perwitasari; Kyes, Randall C.; Iskandar, Entang

doi:10.13057/biodiv/d110201

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…; Perwitasari‐Farajallah et al. ). Microsatellites are the marker of choice for genetic management for a number of reasons including high polymorphic information content and gene diversity.…”

Section: Introductionmentioning

confidence: 99%

“…This is not an error of the zoos but reflects the difficulty of establishing kinship relationships in the absence of genetic data. The use of molecular markers, particularly microsatellite panels, has been shown to be a highly effective genetic management tool in nonhuman primates (Deinard and Kidd 2000;Meier et al 2000;Kanthaswamy et al 2006;Perwitasari-Farajallah et al 2010). Microsatellites are the marker of choice for genetic management for a number of reasons including high polymorphic information content and gene diversity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of North American captive‐born gorillas (Gorilla gorilla gorilla)

Simons

Wagner

Lorenz

2013

Ecology and Evolution

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Western lowland gorillas (Gorilla gorilla gorilla) are designated as critically endangered and wild populations are dramatically declining as a result of habitat destruction, fragmentation, diseases (e.g., Ebola) and the illegal bushmeat trade. As wild populations continue to decline, the genetic management of the North American captive western lowland gorilla population will be an important component of the long-term conservation of the species. We genotyped 26 individuals from the North American captive gorilla collection at 11 autosomal microsatellite loci in order to compare levels of genetic diversity to wild populations, investigate genetic signatures of a population bottleneck and identify the genetic structure of the captive-born population. Captive gorillas had significantly higher levels of allelic diversity (t7 = 4.49, P = 0.002) and heterozygosity (t7 = 4.15, P = 0.004) than comparative wild populations, yet the population has lost significant allelic diversity while in captivity when compared to founders (t7 = 2.44, P = 0.04). Analyses suggested no genetic evidence for a population bottleneck of the captive population. Genetic structure results supported the management of North American captive gorillas as a single population. Our results highlight the utility of genetic management approaches for endangered nonhuman primate species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of North American captive‐born gorillas (Gorilla gorilla gorilla)

Simons

Wagner

Lorenz

2013

Ecology and Evolution

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“…The test was based on amplification of D2S1777 microsatellite marker, a monomorphic DNA microsatellite locus on genetic study of long-tailed and pig-tailed macaques (Perwitasari-Farajallah . 2007;Perwitasari-Farajallah 2010). Allele size in contemporary long-tailed macaques was 160 bp.…”

Section: Macaca Fascicularis M Mulatta Et Al Et Almentioning

confidence: 98%

Testing Reliability of Serum Samples as a DNA Source on Captive Breeding Long-tailed Macaques (Macaca fascicularis)

Perwitasari-Farajallah,

Farajallah

2014

BIOTROPIA

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The serum reliability was tested in captive breeding conditions on the long-tailed macaques ( ). Serum and buffy coat were applied to standard protocol of DNA extraction following the amplification of microsatellite DNA locus of D2S1777 by Polymerase Chain Reaction (PCR) and visualized PCR products by means of Polyacrylamide gel electrophoresis (PAGE) with silver staining. The amount of genomic DNA extracted from serum was sufficient for genotyping individuals at DNA microsatellite locus of D2S1777 with allele size of approximately 160 bp. The study showed that serum could provide reliable alternative for obtaining DNA when taking blood using anticoagulant was impracticable. serum, DNA source, captive breeding, monkeys 1

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Genome-wide mining and comparative analysis of microsatellites in three macaque species

et al. 2017

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Microsatellites are found in taxonomically different organisms, and such repeats are related with genomic structure, function and certain diseases. To characterize microsatellites for macaques, we searched and compared SSRs with 1-6 bp nucleotide motifs in rhesus, cynomolgus and pigtailed macaque. A total of 1395671, 1284929 and 1266348 perfect SSRs were mined, respectively. The most frequent perfect SSRs were mononucleotide SSRs. The most GC-content was in dinucleotide SSRs and the least was in the mononucleotide SSRs. Chromosome size was positively correlated with SSR number and negatively correlated with the relative frequency and density of SSRs. The GC content of chromosome SSRs were negatively correlated with relative frequency of SSRs and GC content of chromosome sequences. The features of microsatellite distribution in assembled genomes of the three species were greatly similar, which revealed that the distributional pattern of microsatellites is probably conservative in genus Macaca. The degenerated number of repeat motifs was found to be different in pentanucleotide and hexanucleotide repeats. Species-specific motifs for each macaque were significantly underrepresented. Overall, SSR frequencies of each chromosome in rhesus macaque were higher than in cynomolgus macaque. The maximum repeat times of mono- to pentanucleotide repeats in cynomolgus macaque was more than other two macaques. These results emphasize the genetic diversity and phylogenetic relationship of genus Macaca species. Our data will be beneficial for comparative genome mapping, understanding the distribution of SSRs and genome structure between these animal models, and provide a foundation for further development and identification of more macaque-specific SSRs.

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Microsatellite DNA polymorphisms for colony management of longtailed macaques (Macaca fascicularis) population on the Tinjil Island

Cited by 3 publications

References 29 publications

Genetic diversity of North American captive‐born gorillas (Gorilla gorilla gorilla)

Genetic diversity of North American captive‐born gorillas (Gorilla gorilla gorilla)

Testing Reliability of Serum Samples as a DNA Source on Captive Breeding Long-tailed Macaques (Macaca fascicularis)

Genome-wide mining and comparative analysis of microsatellites in three macaque species

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